PENGARUH PENAMBAHAN Haematococcus TERHADAP PERTUMBUHAN, TOTAL KAROTEN, VITALITAS DAN LIPID PLASMA KERAPU SUNU (Plectropomus leopardus)

Daniar Kusumawati, Ketut Mahardika, Ketut Maha Setiawati
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Abstract

Benih kerapu sunu D60 merupakan stadia dimana ikan hanya mengkonsumsi pakan buatan dan benih mulai menunjukkan adanya pemudaran warna merah seiring dengan berjalannya waktu. Karoten merupakan pigmen merah, jingga dan kuning yang ditemukan pada tumbuhan, termasuk alga dan buah-buahan. Tujuan penelitian ini untuk mengetahui potensi karotenoid terhadap total karotenoid, laju pertumbuhan, imunitas, total kolesterol dan nutrisi pada benih kerapu sunu. Ikan ukuran panjang total rata-rata 3,19 + 0,2 cm dengan berat total rata-rata 0,42 + 0,07 g diberi perlakuan penambahan karoten Haematococcus pluvialis (NatuRose) 10g/kg pakan (A) dan tanpa karoten sebagai kontrol (B) dengan ulangan 3 kali menggunakan rancangan acak lengkap. Pada akhir penelitian dilakukan uji tantang pada ikan kerapu sunu yang ditempatkan pada wadah berkapasitas 80 L dengan kepadatan 10 ekor dengan ulangan 3 kali. Uji tantang dilakukan dengan menyuntikan 0,1ml inokulum Nervous Necrosis Virus (NNV).Hasil penelitian menunjukkan bahwa benih kerapu sunu perlakuan A memiliki kandungan total karoten (35,74± 0,99 μ/g) lebih tinggi dibandingkan dengan kontrol (30,66 ± 0,21 μ/g) (P value< 0,05). Sementara itu, sintasan setelah uji tantang pada perlakuan A. juga lebih tinggi (53,33 ± 15,28%) dibandingkan dengan kontrol (13,33 ± 15,28%) (P value < 0,05).Perlakuan penambahan karoten dalam pakan tidak berpengaruh nyata terhadap laju pertumbuhan berat maupun panjang juga terhadap total kolesterol. Kandungan kimia pakan lemak (13,14% berat kering) menunjukkan 18,18% lebih tinggi dari kontrol (11,29% berat kering), disamping itu kandungan protein 4,68% lebih rendah dari kontrol.

Keywords

Haematococcus, kerapu sunu, total karoten,vitalitas, nutrisi

References

Aflalo C, Y Meshulam, A Zarka, and S Boussiba. 2007. On the relative efficiency of two vs. one-stage production of astaxanthin by the green alga H. pluvialis pluvialis. Biotechnology and Bioengineering 98 (1), 300 - 305

Barbosa MJ, R Morais, and G Choubert. 1999. Effect of karotenoid source and dietary lipid content on blood astaxanthin concentration in rainbow trout (Oncorhynchus mykiss). Aquaculture 176 (3-4), 331 – 341

Chew BP, BD Mathison, MG Hayek, S Massimino, GA Reinhart, and JS Park. 2011. Dietary astaxanthin enhance immune respone in dogs. Veterinary Immunology and Immunopathology 140 (3 - 4), 199 - 206

Christiansen R, J Glette, Lie, OJ Torrissen, and R Waag. 1995. Antioxidant status and immunity in Atlantic salmon, Salmo salar L., fed semi-purified diets with and without astaxanthin supplementation. Jounal of Fish Diseases 18 (4), 317 - 328

Damiani MC, CA Popovich, D Constela, and PI Leonardi. 2010. Lipid analysis in H. pluvialis pluvialis to assess its potential use as a biodiesel feedstock. Bioresource Technology 101, 3801 – 3807

Gouveia L, P Rema, O Pereira, J Empis. 2003. Colouring Ornamental Fish (Cyprinus carpio and Carassius auratus) With Microalgal Biomass. Aquaculture Nutrition 9 (2), 125-129

Guerin M, ME Huntley, and M Olaizola. 2003. H. pluvialis astaxanthin: applications for human health and nutrition. Trends in Biotechnology, 21 (5), 210 – 216

Higuera-Ciapara I, L Felix-Valenzuela, and FM Goycoolea. 2006. Astaxanthin: A review of its chemistry and applications. Critical Review in Food Science and Nutrition, 46, 185–196.

Hirschberg J. 1999. Production of high-value compounds: karotenoids and vitamin E. Biotechnology 10, 186 - 191

Iwamoto T, K Hosoda, R Hirano, H Kurata, A Matsumoto, W Miki, M Kamiyama, H Itakura, S Yamamoto, and K Kondo. 2000. Inhibition of low-density lipoprotein oxidation by astaxanthin. Journal of Atherosclerosis and Thrombosis 7 (4), 216 – 222

Jyonouchi H, S Sun, and M Gross. 1995. Effect of karotenoids on in vitro immunoglobulin production by human peripheral blood mononuclear cells: astaxanthin, akarotenoid without vitamin A activity, enhance in vitro immunoglobulin production in response to a T-dependent stimulant and antigen. Nutrition and Cancer 23 (2), 171 - 183

Karlsson A. 2001. Signaling For Color Change In Melanophore And A Bioseneor AP.lication. Linköpings University Medical Dissertations. Sweden. P. 53

Kelsh RN. 2004. Review: Pigment Gene Focus. Genetics And Evolution Of Pigment Patterns In Fish. Pigment Cell

and Melanoma Research 17, 326-336

Korzan WJ, RR Robinson, S Zhao, RD Fernald. 2008. Color Change As Potential Behavioral Strategy. Hormones and Behavior 54, 463-470

Kusumawati D dan KM Setiawati. 2016. The use of carotene materials as the source of red color pigmentation on leopard grouper larvae (Plectropomus leopardus). Aquacultura Indonesiana 17 (2), 35 – 45

Li M, W Wu, P Zhou, F Xie, Q Zhou, and K Mai. 2014. Comparison effect of dietary astaxanthin and H. pluvialis pluvialis on growth performance, antioxidant status and immune response of large yellow croaker Pseudosciaena crocea. Aquaculture 434, 227 – 232

Merchie G, E Kontara, P Lavens, R Robles, K Kurmaly, P Sorgeloos. 1998. Effect of vitamin C and astaxanthin on stress and disease resistance of postlarval tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research 29, 579 – 585

Naguib YMA. 2000. Antioxidant activities of astaxanthin and related karotenoids. Journal of agricultural and food chemistry 48, 1150 - 1154

Nery LEM and AML Castrucci. 1997. Pigment Cell Signalling For Physiological Color Change. Comparative Biochemistry And Physiology 118A (4), 1135-1144

Nurpalah R, M Kusmiati, dan K Novitriani. 2017. Pengaruh virgin coconut oil (vco) yang dibuat melalui teknik fermentasi menggunakan bakteri Lactobacillus casei galur komersial yakult terhadap kadar glukosa dan kolesterol darah mencit jantan. Jurnal Kesehatan Bakti Tunas Husada 17 (1), 148-156.

Packer L. 1992. Karotenoids. Academic Press, San Diego,California, USA.

Park JS, JH Chyun, YK Kim, LL Line, BP Chew. 2010. Astaxanthin decreases oxidative stress and inflammation and enhanced immune response in humans. Nutrition and Metabolism 7, 1 – 8

Park JS, BD Mathison, MG Hayek, S Massimino, GA Reinhart, BP Chew. 2011. Astaxanthin stimulates cell-mediated and humoral immune responses in cats. Veterinary Immunology and Immunopathology 144 (3 – 4), 455 - 461

Ranga R, AR Sarada, V Baskaran, and GA Ravishankar. 2009. Identification of karotenoids from green alga H. pluvialis pluvialis by HPLC and LC-MS (APCI) and their antioxidant properties. Journal of Microbiology and. Biotechnology, 19(11), 1333-1341.

Rahman MM, S Khosravi, KH Chang, SM Lee. 2016. Effects of dietary inclusion of astaxanthin on growth, muscle pigmentation and antioxidant capacity of juvenile raibow trout (Oncorhynchus mykiss). Prevention Nutrition and Food Science 21 (3), 281 – 288

Rusdi M, Hasnaeni, dan F Fujaya. 2017. Kadar kolesterol mencit (mus musculus) setelah pemberian kepiting cangkang lunak (Scylla olivaceae). Jurnal Farmasi UIN Alauddin Makassar 5 (2), 84-89

Saha SK, E McHugh, J Hayes, S Moane, D Walsh, P Murray. 2013. Effect of various stress-regulatory factors on biomass and lipid production in microalga H. pluvialis pluvialis. Bioresource Technology 128, 118 - 124

Sugie A, Y Terai, R Ota, N Okada. 2004. The Evolution of Genes for Pigmentation in African Cichlid Fishes. Gene 343, 337-346

Thompson I, G Choubert, DF Houlihan, CJ Secombes. 1995. The effect of dietary vitamin A and astaxanthin on the immunocompetence of rainbow trout. Aquaculture 133 (2): 91 – 102

Tizkar B, A Seidavi, JT Ponce-Palafox, P Pourashoori. 2014. The effect of astaxanthin on reisitance of juvenile prawns Macrobranchium nipponense (Decapoda : Palaemonidae) to physical and chemical stress. Revista de Biología Tropical 62 (4), 1331 – 1341

Yang Y, JM Seo, A Nguyen, TX Pham, HJ Park, Y Park, B Kim, RS Bruno, J Lee. 2011. Astaxanthin-rich extract from the green alga H. pluvialis pluvialis lowers plasma lipid concentration and enhances antioxidant defense in apolipoprotein E knockout mice1-3. Journal of Nutriton 141, 1611 – 1617

Yasir I and JG Qin. 2010. Effect of Dietary Karotenoids on Skin Color and Pigments of False Clownfish, Amphiprion ocellaris, Cuvier. Journal of The World Aquaculture Society 41 (3), 309 - 318

Zhao YY, FF Guan, GL Wang, LL Miao, J Ding, GH Guan, Y Li, and BD Hui. 2011. Astaxanthin preparation by lipase-catalyzed hydrolysis of its esters from H. pluvialis pluvialis algal extracts. Journal of Food Science, 76, 643–650.


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