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KARAKTERISTIK GENETIK LOBSTER MUTIARA (Panulirus ornatus FABRICIUS, 1798) BERDASARKAN MARKA CYTOCHROME OXYDASE SUBUNIT I (COI)
Indriatmoko Indriatmoko, Arip Rahman, Sari Budi Moria Sembiring, Danu Wijaya
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Abstract
Upaya pemulihan lobster mutiara (Panulirus ornatus Fabricius, 1798) melalui restocking dibutuhkan untuk meningkatkan populasinya di alam. Keragaman genetik populasi asli lobster mutiara penting untuk dikaji lebih dahulu sebelum tindakan restocking untuk menghindari introduksi lobster mutiara yang memiliki variasi genetik lebih rendah dibandingkan lobster mutiara yang sudah ada di alam. Penelitian ini bertujuan untuk mengetahui karakteristik genetik lobster pada beberapa lokasi penangkapan lobster mutiara, yaitu Ujung Kulon (n = 10), Pangandaran (n = 13), Tulungagung (n = 14), Banyuwangi (n = 16), dan Lombok Tengah (n = 15). Sampel lobster mutiara dikoleksi pada periode Februari – Oktober 2015. Setiap individu lobster diambil sampel jaringan untuk kemudian dianalisa menggunakan marka COI (Cytochrome Oxidase subunit I). Amplikon sepanjang 700 bp yang diperoleh kemudian dikarakterisasi menggunakan enzim restriksi HinfI, NlaIII, dan TaqI. . Berdasarkan hasil restriksi diperoleh keragaman genetik lobster mutiara tertinggi di Pangandaran (Ho/He = 0,8882). Analisa jarak genetik menunjukan populasi lobster mutiara dari Lombok memiliki jarak genetik terjauh dibanding kan dengan populasi lobster mutiara dari tulungagung-banyuwangi dan ujung kulon-pangandaran (D 0,083 − 0,162).
Kata kunci: COI, genetik, lobster mutiara, PCR-RFLP.
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