Aktivitas Enzim Selulase dari Khamir Candida sp. dan Debaryomyces sp. yang Diisolasi dari Lahan gambut Taman Nasional Bukit Duabelas Jambi

Abstract

ABSTRACT
Cellulalytic Activity of Candida sp and Debaryomyces sp Isolated from Peat Soil of
Bukit Duabelas Natural Park, Jambi. Candida sp. and Debaryomyces sp. Yeast
Degrading cellulose Isolated from Peat Soil in Bukit Duabelas National Park, Jambi. The
objective of study was to investigate the characteristic of yeast that are able to solubilize
cellulose. The yeast was isolated from soil of Tarnan Nasional Bukit Duabelas Jambi.
Out of 2 1 ioslates tested 2 isolates Candida sp. (Isolate J I) and Debaryomyces sp. (Isolate
J2) were able to solubilize Carboxymethyl cellulose. Other isolated soil yeasts were
Rhodotor& sp, and C~ptococcusp . Medium acidity during cell cultivation varied
between 5.8 to 7.2. The CMC-ase activity was 5.9 unit and 5.4 unit for isolate J1 and
isolate 52 respectively. The Km of isolate J1 and isolate 52 were 7.7 x 10" and 8.4 x 1 0-2
(% b/v) respectively. Vmaks of isolate J1 and J2 was 8.28 xlW3 dan 30.66 x lo-) pg
glukosa/ml enzyme /minute, respectively.
Key words: Cellulolytic Yeasts, Candida sp., Debaryomyces sp., Enzymes Activity,
Taman Nasional Bukit Duabelas