Variasi Genetika Kelelawar Genus Aethalops (Chiroptera: Pteropodidae) di Indonesia: Tinjauan dari Gen 12SrRNA pada DNA Mitokondria

Maharadatunkamsi Maharadatunkamsi, M. Syamsul Arifin Zein
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Genetic Variation of Bat in the Genus Aethalops (Chiroptera: Pteropodidae) from
Indonesia: Analysis of 12SrRNA Gene of Mitochondria1 DNA. We report a genetic
variation on 12SrRNA gene to investigate haplotype and nucleotide diversity, as well as
population structure of bats in the genus Aethalops. Samples consisted of 21 individuals
from 7 populations of 5 islands (Sumatra, Jawa, Kalimantan, Bali and Lombok) covering
all islands distribution of this genus in Indonesia. Total DNA was extracted from liver
tissues. Fragment 12SrRNA gene region of the mitochondria1 DNA were amplified by
Polymerase Chain Reaction usingprimers of L 1091 and H 1478. Nucleotide sequences
were determined to investigate genetic variation of this genus. Results indicated that
from 294 bp nucleotide sequences, 33 polymorphic sites with 13 haplotypes were found
among 7 populations.
Key Words: Variation, Bat, Aethalops , Chiroptera, Indonesia, 12SrRNAmitochondrial.

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