PERBANYAKAN TANAMAN BUAH NAGA BERDAGING BUAH MERAH {Hylocereus costaricensis) MELALUI TEKNIK KULTUR JARINGAN

Priyono Priyono
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Abstract

Hylocereus costaricensis is new important fruit in Indonesia. One of constrain in its development is limitation of planting material.The aim of the research is to study the regeneration H. costaricensis through micro shoot induction of node explants. The experiments were carried out in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute. Experiment on microshoots proliferation stage was arranged in a Factorial Completely Randomized Design, with three replications.The first factor was Kinetin concentration consisted of five treatments i.e.: 0, 2.5, 5.0, 7.5 and 10.0 mg/1.The second factor was Indole acetic acid (IAA) concentration consisted of five levels i.e.: 0, 0.25, 0.5, 0.75 and 1 mg/1. Microshoots multiplication stage was arranged in a Factorial Completely Randomized Design,with three replications. The first factor was polyvinyl pyrrolidone (PVP) concentration consisted of six treatments i.e.: 0, 0.25, 0.5, 0.75, 1.0 and 1.25 %. The second factor was Cystein concentration consisted of four treatments i.e.: 0, 25, 50 and 75 mg/1. The microshoots rooting stage the results experiment was laid in a Factorial Completely Randomized Design, with three replications. The first factor was Giberalic acid (GA3) consisted of five treatments i.e.:0, 0.25, 0.5, 0.75 and 1 mg/1. The second factor was Boric acid concentration consisted of four levels i.e.: 0, 50, 100 and 150 mg/1. In the microshoots proliferation stage the results showed that there was interaction between IAA and Kinetin concentration on the microshoots proliferation and the number of shoot per explnat. The best results were obtained from the treatment 0.75 mg/1 IAA + 7.5 mg/1 Kinetin, whereas in this treatment the rate of microshoots proliferation and the number of microshoots perexplant was 50 % and 3.9, respectively. In the microshoots multiplication stage, the results showed that there was interaction between PVP and Cystein concentration. The best results were obtained from the treatment 0.75% PVP + 75 mg/1 Cystein, whereas in this treatment the rate of microshoots multiplication and the number of microshoots per explant was 95% and 6.3, respectively. In the rooting stage, the results showed that there was interaction between GA and Boric acid concentration. The experiment indicated that 0.5 mg/1 GA3 + 100 mg/1 Boric acid showed the best result to stimulate root induction of the in vitro microshoots propagation, whereas in this treatment the percentage of rooted microshoots and the hight of plantlet were 95% and 5.7 Cm, respectively.

Keywords

Kultur jaringan, Hylocereus costaricensis, tunas mikro, zat pengatur tumbuh, antioksidan.

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References

Cailloux F, J Julien-Guerrier, L Linnossier and A Coudret. 1996. Longterm somatic embryogenesis and maturation of somatic embryos in Hevea brasiliensi. Plant Sci. 120,185-196.

Devy NF dan A Sutanto. 1992. Pengaruh Komposisi media dan zat pengatur tumbuh terhadap perbanyakan batang bawah apel asal Bromo secara mikro. J.Hon. 2(4), 13-20.

Gati E, SF Syahid dan I Mariska. 1994. Perbanyakan generatif tanaman vanili melalui kultur in vitro. Bull. Littri. 7, 34-39.

Jones LH, DF Hanke and CJ Euwens. 1995. An evaluation of the role of cytokinin in the development of abnormal enflorescence in oil palm (Elaeis guinensis Jacq) regenerated from tissue culture. J. Plant Growth Regul. 14, 135-142.

Khuri S and J Moorby. 1996. Nodal segment or microtubers as explants for in vitro microtuber production of potato. Plant cell, Tissue and Organ Culture 45, 215-222.

Kristanto, D. 2003. Buah Naga, Pembudidayaan di Pot dan di Kebun. Penebar Swadaya, Jakarta, 8.

Prahardini PER dan T Sudaryono. 1992. Pengaruh kombinasi NAA dan BA terhadap kultur papaya kultivardampit secara in vitro. J.Hort. 2(4), 6-12.

Pierik RLM. 1987. In vitro culture of higher plants. Martinus Nijhoff, Lancaster.

Priyono. 1991. Reproduksi embrio somatic dan pertumbuhan plantlet kopi arabika dalam kultur in vitro. I. Pengaruh sumber eksplan dan komposisi medium. Pelita perkebunan 6(4), 97-102.

Priyono dan S. Danimihardja. 1996. Pengaruh sukrosa dan kasein hidrolisat terhadap reproduksi embrio somatic kopi arabika. Zuriat 7 (1), 22-27.

Priyono dan Sri Winarsih. 2000. Pengaruh arah dan ukuran potongan sisik umbi vanili (Vanila planifolia) terhadap pembentukan tunas mikro dan bulblet secara in vitro. Berita Biologi 5(1), 85-92.

Priyono. 2001. Micropropagation of banana (Musa paradisiaca) through cormlet initiation by in vitro culture of apical meristem slice. Jurnalllmu Dasar 2(1), 36-42.

Shibli RA and Ajlouni MM. 2000. Somatic embryogenesis in.the endemic black iris. Plant Cell, Tissue and Organ Cultured!, 15-21.

Skoog F and Miller CO. 1957. Chemical regulation of growth and organ formation in plant tissue cultured in vitro. Symp.Soc. Exp.Biol. 11, 118-131.

Sri Winarsih dan Priyono. 1995. Induksi tunas aksiler pada kakao secara in vitro. Pelita Perkebunan 11(3), 159-167.

Sri Winarsih, Priyono dan Zaenudin. 1998. Pengaruh zat pengatur tumbuh terhadap perbanyakan kerk lily secara in vitro. Jurnal Hortikultura 8(3), 1145-1152.

Sri Winarsih dan Priyono. 2000. Pengaruh zat pengatur tumbuh terhadap pembentukan dan pengakaran tunas mikro pada asparagus secara in vitro. Jurnal Hortikultura 10(1), 11-17.

Saxena S. 1990. In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Report 9, 437-434.

Saxena S and SS Bhojwani. 1992. In vitro clonal multiplication of 4-year-old plants of the bamboo (Dendrocalamus longispatus KURZ.). Plant Cell Report 11, 135-142.

Purwanti S. 1999. Pengaruh Zat Pengatur Tumbuh Terhadap pertumbuhan bibit vanili hasil perbanyakan kultur biji. Buletin Agro Industri 6, 17-23.

Takayama S and Misawa M. 1982. Regulation of organ formation by cytokinin and auxin in Lilium bulbscale grown in vitro. Plant Cell Physiol. 23, 67-74.

Wachira F and J Ogada. 1995. In vitro regeneration of Camellia sinensis L. by somatic embryogenesis. Plant Cell Rep. 14, 463-466.


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