DEVELOPMENT OF PURE CULTURE STARTER FOR KECAP, AN INDONESIAN SOY SAUCE [Pengembangan Ragi Kecap Khas Indonesia Dengan Biakan Murni]

Joko Sulistyo, Sayuki Nikkuni
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Abstract

In order to prevent aflatoxin contamination during the production of kecap, an Indonesian soy sauce, a white-spored mutant K-IA strain induced from an aflatoxin-negative koji mold was applied to koji making process of kecap. While kecap koji making process took 9 days by conventional method without inoculum, the use of K-IA strain as a starter shortened the process to 3 days at room temperature. The koji prepared with this starter contained 2.3 x 107 cfu/g of Aspergillus sp. K-IA as the dominant speciesand some contaminants from the environment such as other species of Aspergillus (2.0 x 10 cfu/g) and Mucorales (1.4 x 10s cfu/g). The mash prepared with kecap koji inoculated with this starter contained higher formol nitrogen (FN) and water-soluble nitrogen (WN) than those prepared with koji inoculated without any starter.These results demonstrated that the use of this starter did not only contribute to the prevention of aflatoxin contamination but also improved the conventional kecap fermentation process.

Keywords

Aspergillus, ragi kecap, soybean fermentation, non-aflatoxin.

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References

Dharmaputra OS, Tjtrosomo HSS and Sulaswati. 1991. Proceedings of the Twelfth ASEAN Seminar on Grain Postharvest Technology. Naewbanij JO (Ed.). The ASEAN Grain Post-Harvest Programme, Bangkok, Thailand, 110-123

Editorial Committee for Society for Shoyu Shiken-Ho and Japan Soy Sauce Research Institute. 1985. Shoyu Shiken-Ho {Methods of Soy Sauce Studies). Nihon Shoyu Kenkyujo, Tokyo, Japan, 104-105.

Institute of Miso Technologist. 1968. Official Method of Miso Analysis. Institute of Miso Technologist, Tokyo, Japan, 28-29.

Judoamidjojo M. 1986. Memoirs of the Tokyo University of Agriculture, 28, 100-159. Tokyo University of Agriculture, Sakuragaoka, Setagaya-Ku, Tokyo, Japan.

Nikkuni S, Utomo JS, Antarlina SS, Ginting E and Goto T. 2002. Application of white-spored mutants induced from koji molds for the production of Indonesian soy sauce {kecap). Mycotoxins 52 (1), 13-22.

Sardjono, Kapti R and Sudarmadji S. 1992. Growth and aflatoxin production by Aspergillus flavus in mixed culture with Aspergillus oryzae. ASEAN Food J.I, 30-33.


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