Harmastini Sukiman, Sylvia Lekatompessy, Tiwit Widowati
| Abstract views: 334 | PDF views: 839


Batang Gadis National Park (TNBG) is one of the tropical forests in North Sumatra with megabiodiversity of flora and fauna including microorganism. Endophytic microbes conservation from varieties of forest plants in Batang Gadis National Park has been completed. Nineteen endophytes isolates from TNBG have been screened for their potential on producing bioactive compound against the pathogenic bacteria. Qualitative screening has been done using the growth agar media and identified the clear zone appeared surrounding the bacteria colony. The result indicated that isolate MSCI 87.4 showed high strengthening secresion (4.35) againts Xanthomonas campestris, whereas isolate MSCI 37.1 showed high secretion against Bacillus subtilis (2.69) and Escherichia coli (2.60). Isolate MSCI 37.4 showed potential on producing bioactive compound againts Staphylococcus aureus (4.41). Isolates MSCI 87.4, MSCI37.1, MSCI 37.4 and MSCI 58.1 even could produce bioactive compound against four pathogenic bacteria that are Xanthomonas campestris, Bacilus subtilis, Escherichia coli and Staphylococcus aureus, whereas isolate MSCI 15.5b potential on producing bioactive compound against two isolates namely Xanthomonas campestris and Bacillus subtilis. The endophytes bacteria mainly belong to the Gram negative group and four out of nineteen isolates tested belong to the Gram positive group. The cell mainly coccus and only one is bacilli without fiagella. The five most potential isolates has been maintained under freeze dried condition for futher conservation and study. Thin Layer Analysis using semipolar organic separation solution showed that most isolates identified were able to produce bioactive compound except two isolates i.e. MSCI 48.4a and MSCI 53.1; however further analysis is needed to confirm the product.


Mikroba endofitik, Xanthomonas campestris, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, KLT

Full Text:



Breger J, BB Fuch, G Aperis, TI Moy, FM Ausubel and E Mylonakis. 2007. Antifungal chemical compound identified using a C. elegans pathogenic assay. Plos Pathogens 3, Issue 2.el8.

Clay K. 1988. Fungal endophytes of grasses and defensive mutualism between plants and fungi. Ecology 69, 10 - 16.

Fabry W, PO Okemo and R Ansorg. 1998. Antibactery activity of East African medicinal plants. J. Enthopharmacol 60,79 -84.

Lumyong S, P Niamsup, D Pongputachart, P Lumyong and F Tomita. 1997. Endophyte from Thailand: isolation and screening for tartaric acid and oligosaccharide production. Ini Biotechnology for Sustainable Utilization of Biological Resources in The Tropics 12, 394-403. Nakhon Ratcshima, Thailand, 19-22 November 1997.

Lumyong S, B Bussaban, P Lumyong, KD Hyde, EHC Mckenzie, K Asano and F Tomita. 2004. Evaluation of endophytic fungi from Zinggiberaceae for antibiotic production la: Biotechnology for Sustainable Utilization of Biological Resources in The Tropics 17, 472-476. Bali, Indonesia, 3-4 Desember 2004.

M Tanaka, K Asano, M Yoshimura, T Sone, M Suto, A Yokota, E Sukara and F Tomita. 2001. The production of lepidimoide by endophytic fungus from polysaccharide extracted from Abelmoshus sp. -Identification of the product and the producing organism. In; Biotechnology for Sustainable Utilzation of Biological Resources in The Tropics IS, 82-87. Bangkok, Thailand, 7-9 November 2001.

Ruth M, DN Widyaningrum, A C Djohan and H Sukiman. 2006. Pengkajian bakteri endofit penghasil senyawa bioaktif untuk proteksi tanaman. Biodiversitas 7(3), 221-224.

Radu S, CY Kqueen, M Ismael Abdul Karim, M Suto, M Tanaka and F Tomita. 2001. Endophyte fungi prospecting from medicinal plants in Malaysia for useful subtances. In: Biotechnology for Sustainable Utilization of Biological Resources in The Tropics 15, 77-81. Bangkok, Thailand, 7-9 November 2001.

Saranpuetti C, M Tanaka, T Anindyawati, K Asano, A Yokota and F Tomita. 2004. Endophytes new potential resources for useful enzymes. In.: Biotechnology for Sustainable Utilization of Biological Resources in The Tropics 17, 426-433. Bali.Indonesia, 3-4 Desember 2004.

Sukara E, R Melliawati and S Saono. 1992 Amylases production from cassava by an indigenous yeast. J.Sci. Tech. Develop 9(1), 157-68.

Sukiman H. 2004. Potensi mikroba endofitik Taman Nasional Batang Gadis. Majalah Tropika Indonesia. 8(4), 40-41.

Wilson D. 2000. Ecology of woody plant endophytes. In: CW Bacon and JF White Jr. Microbial Endophytes. Marcell Dekker Inc, New York.


  • There are currently no refbacks.