PRODUKSI KITINASE EKSTRASELULER Aspergillus rugulosus 501 SECARA OPTIMAL PADA MEDIA CAIR

Nunuk Widhyastuti
10.14203/beritabiologi.v8i6.839
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Abstract

Chitinases hydrolyzing chitin are produced by various organisms and their physiological functions depend on their sources.Chitinases and its hydrolysis products have a broad range of applications. Increasing commercial interest in chitinase and its products has led to the need of inexpensive, reliable sources of active and stable chitinase preparations.The activity of chitinase produced by Aspergillus rugulosus 501 in liquid culture and the parameters that controlled the enzyme synthesis were studied to find optimal conditions for the enzyme production. Hydrolysis products of chitinase were detected by Reissig method. The results showed that fungal chitinase activity in media containing organic nitrogen and phosphate was higher than that in media containing trace element, organic and anorganic nitrogen but lack of phosphate. The maximum enzyme activity reached in the second day incubation.Chitinase production was affected by the initial pH of media, chitin consentration and source of nitrogen, while the consentrasion of inoculum used in this study did not affect enzyme production. High enzyme activity was found in media with initial pH 5.0-6.5. When using different consentration of colloidal chitin, chitinase activities were also found high in concentration from 0.75% tol .5%. The organic nitrogen sources gave the better results compared to the anorganic ones.

Keywords

Kitinase, produksi, koloidal kitin, Aspergillus rugulosus 501.

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References

Bhattacharya D, A Nagpure and RK Gupta. 2007. Bacterial Chitinases: Properties and Potential. Critical Reviews in Biotechnology 27,21 -28.

Binod P, C Sanhya, P Suma, G Szakacs and A Pandey. 2007. Fungal Biosinthesis of Endochitinase and Chitobiase in Solid State Fermentation and Their Application for The production of /V-acetyl-Dglucosamine from Colloidal Chitin. Bioresource Technology 98,2742-2748.

Dahiya N, R Tewari, RP Tiwari and GS Hoondal. 2005. Chitinase from Enterobacter sp. NRG4: Its Purification, Characterization and Reaction Pattern. Electronic Journal of Biotechnology 8(2), 134-145.

Dahiya N, R Tewari and GH Hoondal. 2006. Biotechnological Aspects of Chitinolytic Enzymes: A Review. Applied Microbiology and Biotechnology 71(6), 773-782.

Gohel V, A Singh, M Vimal, P Ashwini and HS Chhatpar. 2006. Bioprospecting and Antifungal Potential of Chitinolytic Microorganism. African Journal of Biotechnology 5(2), 54-72

Ogawa K, N Yoshida, K Kariya, C Ohnishi and R Ikeda. 2002. Purification and Characterization of a Novel Chitinase from Burkholderia cepacia Strain KH2 Isolated from The Bed Log of Lentinus edodes, Shitake Mushroom. Journal of General and Applied Microbiology 48,25-33.

Omumasaba CA, N Yoshida and K Ogawa. 2001. Purification and Characterization of Chitinase from Trichoderma viride. Journal of General and Applied Microbiology 47,53-61.

Patil RS, V Ghormade and MV Deshpande 2000 Chitinolytic Enzymes: An Exploration. Enzyme and Microbial Technology 26, 473-483.

Rattanakit N, A Plikomol, S Yano, M Wakayama and T Tachiki. 2002. Utilization of Shrim Shellfish Waste as a Substrate for Solid-State Cultivation of a Culture Based on Chitinase Formation Which Is Necessary for Chitin Assimilation. Journal of Bioscience and Bioengineering 93(6), 550-556.

Reissig JL, JL Strominger and LF Leloir. 1955. A Modified Colorimetric Method for The Estimation of iV-acetylamino Sugars. Journal of Biological Chemistry 217, 959-966.

Sandhya C, LK Adapa, KM Nampoothiri, P Binod, G Szakacs and P Ashok. 2004. Extracellular Chitinase Production by Trichoderma harzianum in Submerged fermentation. Journal of Basic Microbiology 44(1), 49-58.

Sashiwa H, S Fujishima, N Yamano, N Kawasaki, A Nakayam, E Muraki, K Hiraga, K Oda and S Aiba. 2002. Production of JV-acetyl-D-glucosamine from cc-chitin by Crude Enzymes from Aeromonas hydrophila H-2330. Carbohydrate Research 337, 761-763.

Scigelova M and DGH Crout. 1999. Microbial Nacetylhexosaminidase and Their Biotechnological Applications. Enzyme and Microbial Technology IS, 3-14.

Souza RF, RMA Soares, RP Nascimento, RRR Coelbo and RC Gomes. 2005. Effect of Different Carbon Sources on Endochitinase Production by Colletotrichum gloeosporioides. Current Microbiology 51,16-21.

Suhartono MT. 1989. Enzim dan Bioteknologi. Bogor: PAU BioteknologilPB.

Vaidya R, P Vyas and HS Chhatpar. 2003. Statistical Optimization of Medium Components for The Production of Chitinase by Alcaligenes xylosoxydans. Enzyme Microbial Technology 33,92-96.

Wasli AS, MM Salleh and RM Illias. 2006. Production and Characterization of Crude Chitinase from Trichoderma virens. Regional Post Graduate Conference on Engineering and Science (RPCES), Johore 26-27 July.

Xia G, C Jin, J Zhou, S Yang, S Zhang and C Jin. 2001. A No vel Chitinase Having A Unique Mode of Action from Aspergillus fumigatus YJ-407. European Journal of Biochemistry 268,4079-4085.

You-Jin J, F Shahidi and K Se-Kwon. 2000. Preparation of Chitin and Chitosan Oligomers and Their Appications in Physiological Functional Foods. Food Reviews International 16(2), 159-176.

DOI: https://doi.org/10.14203/beritabiologi.v8i6.839

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