KERAGAMAN FENOTIPE RAPD Santalum album L. DIPULAU TIMOR BAGIAN TIMUR

Yuyu S Poerba, Albert H Wawo, KS Yulita
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Abstract

Santalum album L. (sandalwood/cendana) is known as one of medicinal and aromatic tree species in Indonesia. The species is valued for its quality light wood timber and for its medicinal properties.The species has been overexploited and is considered as vulnerable plant species.The present study aimed to assess genetic diversity and to estimate genetic relationship among 58 accessions of plant germplasm collection using Random Amplified Polymorphic DNA (RAPD).Two RAPD primers generated 34 scorable bands with 97.06% of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method.The range of genetic dissimilarity value among species was from 6% to 91%, while the range of genetic distance between populations was from 1.89% and 26.88%.These values showed that 5.album from Eastern part of Timor was genetically diverse populations.Within the 12 populations, there were 9 banding patterns recorded from primer OPA 16 and 12 banding patterns from primer OPB 12, suggesting that OPB 12 was more sensitive than that of OPA 16 to show variation within the sample used.

Keywords

Cendana, Santalum album L., RAPD, keragaman genetik.

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References

Delaporta, SL, Wood J and Hicks JB. 1983. A plant DNA minipreparation. Version II. Plant Molecular Biologyn Reporter 4, 19-21.

Dunn G and Everitt BS. 1982. An Introduction to Mathematical Taxonomy. Cambridge University Press, Cambridge.

Fox JED, Brand JE, Barret DR and Effendi M. 1994. Genetic variation in Santalum album in Timor. Proceedings of a Regional Workshop for Pacific Island Countries, 93-110.

Gjerum L, JED Fox and Y Ehrhart (Editors) CIRAD, ACIAR & FAO Hayati PKD, Hartana, A, Suharsono dan Aswidinnoor H. 2001. Keanekaragaman genetikakelapa'Genjah Jombang' berdasarkan Random Amplified Polymorphic DNA. Hayati 7, 35-40.

Islam MA, Kloppstech K and Esch E. 2005. Population genetic diversity of Curcuma zedoaria (Christm.) Roscoe- a conservation prioritized medicinal plant in Bangladesh. Conservation Genetics 6,1027-1033.

Mogea, JP, Gandawidjaja D, Wiriadinata H, Nasution RE danlrawati. 2001. Tumbuhan Langka Indonesia. Pusat Penelitian dan Pengemnagan Biologi

Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small numbers of individuals. Genetics 89, 583-590.

Page RDM. 1996. TREEVIEW: An application to display phylogenetic trees onpersonal computers. Computer Applications in the Biosciences 12, 357- 358

Parjanto SM, Artama WT dan Purwantoro A. 2006. Indentifikasi penanda RAPD untuk penentuan jenis kelamin salak (Salacca zalacca Gart. Voss). Berkala Ilmiah Biologi 5, 57-63.

Pither R, Shore JS and Kellman M. 2003. Genetic diversity of the tropical tree Terminalia amazonia (Combretaceae) in naturally fragmented populations. Heredity 91, 3017-313.

Poerba YS. 2003. Penampilan karakter agronomi dan analisis Random Amplified Polymorphic DNA (RAPD) genotipe mutan Sonchus arvensis L. Buku Kumpulan Abstrak Seminar Nasional X Persada, Jakarta 4 Juli 2003,124. Persada Cabang Bogor dan Badan Pengurus Pusat Persada.

Poerba, YS. 2005. Studi keragaman genetic Santalum album (L.) berdasarkan marka Random Amplified Polymorphic DNA. Laporan Teknik Pusat Penelitian Biologi LIPI2005,695-701.

Rahayu S, Wawo AH, van Noordirjk M dan Hairiah K. 2002. Cendana:Deregulasi danStrategi Pengembangannya, 60. World Agroforestry Center, ICRAF, Bogor.

Rath P, Rajaseger G, Goh CG and Kumar P. 1998. Phylogenetic analysis of Dipterocarps using Random Amplified Polymorphic DNA markers. Annals of Botany 82, 61-65.

Rimbawanto A, Widyamoko AYPBC, dan Sulistyowati P. 2006. Distribusi keragaman genetika populasi Santalum album berdasarkan penanda RAPD. Jurnal Penelitian Hutan Tanaman 3,175-181.

Rohlf FJ. 1993. NTSYS-pc Numerical Taxonomy and Multivariate Analysis. Versionl.80.Applied Biostatistics Inc.

Shashidhara G, Hem a MV, Koshy B and Farooqi AA. 2003. Assessment of genetic diversity and identification of core collection in sandalwood germplasm using RAPDs. Journal of Horticultural Science & Biotecnology 78,528-536.

Siregar UJ, Sudarmonowati E and Hartati NS. 1998. Development of RAPD protocol for Shorea laevis. Annales Bogorienses 5, 85-92.

Soekotjo. 2001. Konservasi ex-situ cendana {Santalum album L.): Aplikasi dan tantangannya. Berita Biologi Edisi Khusus Masalah Cendana NTT 5,515-519.

Telles MPC, Coelho ASG, Chaves LJ, Diniz-Filho JAF and D'Ayala Valva F. 2003. Genetic diversity and population structure of Eugenia dysenterica DC. (cagaiteira - Myrtaceae) in Central Brazil: spatial analysis and implications for conservation and management. Conservation Genetics 4,685-695.

TingeySV, RafalskiJAandHanafeyMK. 1994. Genetic analysis with RAPD markers. Dalam: Plant Molecular Biology. C. Coruzzi and P. Puidormenech (Eds.), 491-498.

Widjaja EA dan Poerba YS. 2004. Pengumpulan data plasma nutfah dan genetika. Dalam: Pedoman Pengumpulan Data Keanekaragaman Flora. Rugayah, Widjaya EA dan Praptiwi (Editor), . 113-140. Pusat Penelitian Biologi -LIPI.

Williams JG, Kubelik AR, Livak KJ, Rafalsky JA and Tingev SV. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research 18,6531-6535.

Wiriadinata, H. 2001. Tumbuhan. Dalam: Jenis-jenis Hayati yang Dilindungi Perundang - undangan Indonesia. Noerdjito, M. and I. Maryanto (Eds). 221. Balitbang Zoologi (Museum Zoologicum Bogoriense) Puslitbang Biologi-LIPI & The Nature Conservancy.

Yeh FC, Yang RC and Boyle T. 1999. Popgene Version 1.31. Microsoft Windows-based freeware for population genetic analysis. Available at:h 11 p ://www.ualberta.ca/~fyeh/download.htm.


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