KRIOPRESERVASI TANAMAN PURWOCENG {Pimpinella pruatjan Molk.) DENGAN TEKNIK VITRIFIKASI

I Roostika, I Darwati, R Megia
| Abstract views: 946 | PDF views: 1331

Abstract

Pruatjan (Pimpinellapruatjan Molk.) is an Indonesian endangered medicinal plant that included in Appendix I based on CITES. Therefore it is a highly protected species. To avoid extinction of this plant, it is very important to conserve the plant. In vitro conservation is more suitable since this plant is difficult to be cultivated outside of its habitat. Cryopreservation technique may conserve this material for a long-term period. The objectives of this research were to find optimized treatments for pre culture, loading, and dehydration on cryopreservation of pruatjan. The research was conducted at Tissue Culture Laboratory in Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, started from May to November 2007. Pre culture was conducted using DKW basal media that added by sucrose at the level of 0.3,0.4, and 0.5M for one and three days incubation. Loading was conducted in DKW basal media containing 2M glycerol and 0.4M sucrose for 15,30, and 45 minutes duration time. Dehydration was conducted in several cryoprotectants, namely PVS1 (22% glycerol + 13% propy lene glycol + 13% etylene glycol + 6% DMSO + 3% sucrose), PVS2 (30% glycerol + 15% etylene glycol + 15% DMSO + 0,4M sucrose), PVS3 (50% glycerol + 50% sucrose), and PVS4 (35% glycerol + 20% etylene glycol + sucrose 0.6M). Result showed that pruatjan could be preserved through cryopreservation by vitrification method. The best pre culture was using 0.3 M sucrose for one day, the best loading was 30 minutes, while the best cryoprotectant was PVS2 with 90% success before freezing and 40% after freezing. The success may be improved by applying pre growth treatment, optimizing temperature of thawing, modification of recovery media and incubation condition.

Keywords

Kriopreservasi, purwoceng, pruatjan, Pimpinella pruatjan Molk., vitrifikasi.

Full Text:

PDF

References

Ashmore SE. 1997. Status Report on The Development and Application of In-Vitro Techniques for The Conservation and Use of Plant Genetic Resources, 67. IPGRI, Queensland, Australia.

Bachiri Y, GQ Song, P Plessis, A Shoar-Ghaffari, T Rekab and C Morisset. 2001. Routine Cryopreservation of Kiwifruit (Actinidia spp.) Germplasm by Encapsulation-Dehydration: Importance of Plant Growth Regulators. CryoLetters 22, 61-74.

Bajaj YPS. 1979. Technology and Prospects of Cryopreservation of Germplasm. Euphytica 28,267-285.

Benson EE, PT Lynch and J Jones. 1992. Detection of lipid peroxidation products in cryoprotected and frozen rice cells: consequences for post-thaw survival. Plant Science 110,249-258.

Bhojwani SS and MK Razdan. 1983. Plant Tissue Culture: Theory and Practise, 502. Elsevier. Amsterdam. New York.

Driver JA and AH Kuniyuki. 1984. In vitro propagation of paradox walnut rootstock. Hort. Science 19, 507-509.

Dumet D and EE Benson. 2000. The use of physical and biochemical studies to elucidate and reduce cryopreservation-induced damage in hydrated/ desiccated plant germplasm. In: F Engelmann and H Takagi (Eds.). Cryopreservation of Tropical Plant Germplasm: Current Research Progress and Application. 43-S6.

IPGRL RamsAtaty. Gnanapragasam S and IK Vasil. 1992. Cryopreservation of Immature Embryos, Embryonic Callus and Cell Suspension Cultures of Gramineous Species. Plant Science 83,205-215.

Grout BWW. 1995. Introduction to the In Vitro Preservation of Plant Cells, Tissues and Organs. In: B Grout (Ed.). Genetic Preservation of Plant Cells In Vitro, 1-17. Springer Lab Manual Berlin-Heidelberg.

Hirai D and Sakai A. 1999a. Cryopreservation of In Vitro- Grown Meristems of Potato (Solanum tuberosum L.) by Encapsulation-Vitrification. Potato Research 42, 153-160.

Hirai D and A Sakai. 1999b.

Cryopreservation of In Vitro-Grown Axillary Shoot Tip Meristems of Mint (Mentha spicata L.) by Encapsulation-Vitrification. Plant Cell Report 19,150-155.

Kartha KK. 1985. Meristem culture and germplasma preservation. In: KK Kartha (Ed.). Cryopreservation of Plant Cells and Organics. 116-134. Cre Press. Florida,.

Leunufna S. 2004. Improvement of the in vitro maintenance and cryopreservation of yams (Dioscorea spp.), 120. Martin-Luther-Universitat Halle-Wittenberg, Berlin.

Matsumoto T, A Sakai and K Yamada. 1994. Cryopreservation of In Vitro-Grown Apical Meristems of Wasabi (Wasabi japonica) by Vitrification and Subsequent High Plant Regeneration. Plant Cell Report 13, 442-446.

Panis B, H Schoofs, S Remy, L Sagi and R Swennen.2000. Cryopreservation of Banana Embryogenic Cell Suspensions: An Aid for Genetic Transformation. In: F Engelmann and H Takagi (Eds.). Cryopreservation of Tropical Plant Germplasm: Current Research Progress and Application, 103-109. IPGRI. Rome-Italy.

Pennycooke JC and LE Towill. 2000. Cryopreservation of shoot tips from in vitro plants of sweet potato (Ipomea batatas (L.) Lam.) by vitrification. Plant Cell Reports 19, 733-737.

Pennycooke JC and LE Towill. 2001. Medium alterations improve regrowth of sweet potato (Ipomea batatas (L.) Lam.) shoot tips cryopreserved by vitrification and encapsulation-dehidration. CryoLetters 22,381-389.

Reinhoud PJ, FV Iren, and JW Kijne. 2000. Cryopreservation of Undifferentiated Plant Cells.In: F Engelmann and H Takagi (Eds.). Cryopreservation of Tropical Plant Germplasm: Current Research Progress and Application, 91-102. IPGRI. Rome-Italy.

Rifai MA, Rugayah dan EA Widjaja. 1992. Thirty Years of The Eroded Species Medicinal Crops. Floribunda 28.

Rahardjo M. 2003. Purwoceng Tanaman Obat Aprodisiak yang Langka. Warta Penelitian dan Pengembangan Tanaman Industri 9, 4-7.

Roostika I dan I Mariska. 2004. Pemanfaatan Teknik Kriopreservasi dalam Penyimpanan Plasma Nutfah Tanaman. Buletin Plasma Nutfah 9,10-18.

Roostika I, I Mariska, GA Wattimena dan N Sunarlim. 2004. Penerapan teknik vitrifikasi pada penyimpanan ubi jalar (Ipomea batatas (L) Lam.) secara kriopreservasi. JurnalPenelitian Pertanian 23,117-122.

Sakai A, S Kobayashi and I Oiyama. 1990. Cryopreservation of Nucellar Cells of Navel Orange (Citrus sinensis Osb. Var Brasiliensis Tanaka) by Vitrification. Plant Cell Report 9,30-33.

Sakai A. 1993. Cryogenic Strategies for Survival of Plant Culture Cells and Meristem Cooled to -196CC. In: A. Sakai (Ed.). Cryopreservation of Plant Genetic Resources, 5-26. Japan International Cooperation Agency. Japan.

Syahid SF, O Rostiana dan M Rohmah. 2005. Pengaruh NAA dan IBA terhadap perakaran pruatjan (Pimpinella alpina Molk.) in vitro. Jurnal Penelitian Tanaman Industri II, 146-151.

Takagi H, NT Thinh, OM Isulam, T Senboku and A Sakai. 1997. Cryopreservation of In Vitro-Gtovm Shoot Tips of Taro (Colocasia esculenta L. Schott) by Vitrification. Plant Cell Report 16, 594-599.

Towill LE and RL Jarret. 1992. Cryopreservation of Sweet Potato (Ipomea batatas (L.) Lam.) Shoot Tips by Vitrification. Plant Cell Report 11,175-178.

Withers LA. 1980. Preservation of Germplasm in Perspectives in Plant Cell and Tissue Culture. In: IK Vasil(Eds.).f. Cytol. Suppl, 101-136. IRL Press. Washington.


Refbacks

  • There are currently no refbacks.