Tjandra Chrismadha, Lily M Panggabean, Yayah Mardiati
| Abstract views: 3228 | PDF views: 3811


An experiment was carried out to find out the optimum nitrogen and phosphorous concentration for growth and phycocyanin production in Spirulina fusiformis culture. The cultures were grown in Zarouk medium at various nitrogen and phosphorous concentrations, which were 0.0 mM N, 7.5 mM N, 15.0 mM N, 22.5 mM N, and 30.0 mM N, as well as 0 raM P, 90 mM P, 180 mM P, 270 mM P, and 360 mM P, with four replications each. The result shows that optimal growth of the alga, which is expressed in terms of the biomass yield, was achieved at nitrogen and phosphorous concentration of 7.5 mM and 270 mM, respectively.At the same time, the highest phycocyanin content was obtained at nitrogen concentration of 22.5 mM, which was 1,2% of the biomass, and phosphorous concentration of 360 mM, which was 1.1% of the biomass. According to this result, it is suggested the optimum concentration of nitrogen and phosphorous in the media of Spirulina culture for phycocyanin production is 22.5 mM and 360 mM, respectively.


Alga, fikosianin, fosfor, nitrogen, Spirulina fusiformis.

Full Text:



Borowitzka MA. 1988. Algal media and sources of algal culture. In: Microalgal Biotechnology. MA Borowitzka and LJ Borowitzka (Eds), 456-465. Cambridge University Press. Cambridge.

Boussiba S and Richmond A. 1979. Isolation and purification of phycocyanins from the blue-green alga Spirulina platensis. Archives of Microbiology 120,155-159.

Boussiba S and Richmond A. 1980. c-Phycocianin as a storage protein in the blue-green alga Spirulina platensis. Archives of Microbiology 125, 143 - 147.

Chrismadha T. 1994. Growth and lipid production of Phaeodactylum tricornutum in a tubular photobioreactor. Master Thesis, Murdoch University, Perth, Western Australia, 211 pp.

Chrismadha T and Borowitzka MA. 1994. Effect of cell density and irradiance on growth, proxymate composition and eicosapentanoic acid production of Phaeodactylum tricornutum grown in a tubular photobioreactor. Journal ofPhycology 6, 67-74.

Cortes MCC, Sly LI and Doelle HW. 1997. The effect of nitrate concentration on phycocyanin production by Spirulina platensis UTEX 2340. Proceeding of The 2nd Asia-Pacific Marine Biotechnology and 3rd Asia-Pacific Conference on Algal Biotechnology, Phuket, Thailand, 7-10 May 1997, 261-264.

Goldman JC. 1979. Outdoor algal mass culture. II. Photosynthetic yield limitations. Water Research 13,119—136.

Jeffrey SW and Humprey GF. 1975. New spectrophotometric equation for determining chlorophyll a, b,cl, and c2 in higher plants, algae and natural phytoplankton. Biochemie und Physiolgie der Pflanzen 1967,191-194.

Kochert G. 1978. Carbohydrate determination by phenolsulphuric acid method. In: Handbook ofPhycological Methods: Physiological and Biochemical Methods. JA Hellebust and JS Craigie (Eds), 95-75. Cambridge University Press. Cambridge.

Lowrey OH, Rosenbrough NJ, Farr ALand Randall RJ. 1951. Protein measurement with the folin-phenol reagent. The Journal of Biological Chemistry, 1983, 265-275.

Piorreck M and Pohl P. 1984. Formation of biomass, total protein, chlorophylls, lipids, and fatty acids in blue green algae during one growth phase. Phytochemistry, 23,217-223.

Reddy CM, Bhat VB, Kinarmay G, Redding MN, Reddana P and Mediastla KM. 2000. Selective inhibition of cyclooxygenase-2 by c-phycocyanin , a billiprotein from Spirulina platensis. Biochemical and Biophysical Research Communication 277,597-603.

Richardson B, Orcutt DM, Schwertner HA, Martinez CL and Wickline HE. 1969. Effects of nitrogenlimitation on the growth and composition of unicellular algae in continuous culture. Applied Microbiology, 18,245-250.

Richmond A. 1988. Spirulina. In: Microalgal Biotechnology. MA Borowitzka and LJ Borowitzka (Eds), 85-121. Cambridge University Press. Cambridge.

Roessler PG 1988. Effect of silicon deficiency on lipid composition and metabolism in diatom Cyclotella nana. Journal ofPhycology, 24, 394-400

Romay C, Armesto J, Remirez D, Gonzalez R, Ledon N and Garcia I. 1998. Antioxidant and antiinflammatory properties of c-phycocyanin from blue-green algae. Inflammatory Research, 47(1), 36-41.

Romay C, Gonzalez R, Ledon N, Remirez D and Rimbau V. 2003. c-Phycocyanin: Abiliprotein with antioxidant, anti-inflammatory and neuroprotective effects. Current Protein andPeptide Science, 4(3), 207-216.

Sukenik A. 1991. Ecophysiological consideration in optimization of eicosapentanoic acid production by Nannochloropsis sp. (Eustigmatophyceae). Bioresource Technology 35,263-269.

TaguchiS, HirataJA, and Laws EA. 1987. Silicate deficiency and lipid synthesis of marine diatoms. Journal ofPhycology 23, 260-267.

Tedesco MA and Duerr EO. 1989. Light, temperature, and nitrogen starvation effects on the total lipid and fatty acid content and composition of Spirulina platensis UTEX 1928. Journal of Applied Phycology 1,201-209.

Thomas WH, Siebert DLR, Alden M, Neori A and Eldridge P. 1984. Yield, photosynthetic efficiencies and proximate composition of dense marine microalgal cultures. I. Introduction and Phaeodactylum tricornutum experiments. Biomass 5,181-209.

Tornabene TG, Bourne TF, Raziuddin S and Ben- Am otz A. 1985. Lipid and lipopolysaccharide constituents of cyanobacterium Spirulinaplantensis (Cyanophyceae, Nostocales). Marine Ecology Progress Serries 22, 121-125.

VonshakAand Richmond A. 1985. Problems in developing the biotechnology of algal mass production. Plant andSoil 89, 129-135.


  • There are currently no refbacks.