INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe

Djadja Siti Hazar Hoesen, Witjaksono Witjaksono, LA Sukamto
10.14203/beritabiologi.v9i3.790
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Abstract

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator

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References

Aswath CR. 2001. Callus induction and somatic embryogenesis of Dendrobium Queen Sonia. Proceeedings of APOC7, 60-62. Nagoya Japan.

Aprianita RR, Esyanti dan AH Siregar, 2003. Pengaruh pemberian elisitor jamur Phytium aphanidermatum (Edson) Fitzp. Terhadap kandungan ajmalisin pada kultur kalus berakar Catharanthus roseus (L.) G. Don. Berita Biologi 6(4), 546-547.

Anonim. 2005. Anggrek Dendrobium. Trubus Info Kit 1(1). Trubus Swadaya. Cimanggis, Depok.

Borthakur M, J Hazarika and RS Singh. 1999. A protocol for micropropagation of Alpinia galanga. Plant Cell, Tissue and Organ Culture 55, 231-233.

Friedman R, A Altman and Bahcrachu. 1985. Polyamines and root formation in mung bean hypocotyl cuttings. II. Incorporation of precursor into polyaminers. Plant Physiology 79, 80-83.

Gandawidjaja D dan S Sastrapradja, 1980. Plasmanutfah Dendrobium asal Indonesia. Buletin Kebun Raya 4(4),113-125.

Gamborg OL and JP Shyluk, 1981. Nutrition, media and characteristic of plant cell and tissue culture, 21-42. In: Plant Tissue Culture Methods and Application in Agriculture. TA Thorpr (Ed.). Academic Press, New York.

Gunawan LW. 1988. Teknik Kultur Jaringan. Pusat Antar Universitas, Bioteknologi IPB. Bogor.

George EF. 1993. Plant Propagation by Tissue Culture in Practice. Part 1. Exegetic Limited England.

Ganapathi TR, NS Higgs, PJ Balint-Kurti, CJ Artnzen, GD May and JM Van Eck. 2001. Agrobacteriummediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB). Plant Cell Reports 20, 157-162. Springer-Verlag.

Hoesen DSH. 1983. Kemampuan silang antar jenis dalam section Ceratobium. Laporan Penelitian Tahun 1982-1983. Sumber Bahan Industri. Lembaga Biologi Nasional-LIPI. Tidak dipublikasikan.

Irawati. 1996. Potensi anggrek spesies Indonesia dan pengembangan kultivar unggul. (publikasi terbatas). Makalah Disampaikan pada Seminar Anggrek, 1-2 Nopember 1996, di Jakarta. Perhimpunan Anggrek Indonesia (PAI).

Irawati. 2000. Diferensiasi berbagai macam eksplan pada perbanyakan Philodendron goeldii (Araceae) secara in vitro. Berita Biologi 5(1), 69-75.

Lavarack B, V Harris and G Stocker, 2002. Dendrobium and Its Relatives. Timber Press. Portland, Oregon USA.

Lugrayasa IN and DSH Hoesen, 2006. Dendrobium Sw. tanaman hias dalam ruangan. PROSEA Sumber Daya Nabati No. 20.1. Pusat Penelitian Biologi-LIPI. LIPI Press.

Murashige T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15 : 473 - 497.

Murashige T. 1975. Plant propagation through tissue culture. Ann. Rev. Plant Physjol. 25, 135-166

Mukhri Z; A Baihaki dan P Sudigdo. 1985. Kultur jaringan temulawak {Curcuma xanthorrhiza Roxb. - Zingiberaceae) dan studi awal kemungkinan penggunaan mutagen untuk meningkatkan kadar kurkuminnya. Prosiding Simposium Nasional Temulawak, 167-172.

Lembaga Penelitian Universitas Padjadjaran. Mandegaran Z and VK Sieber. 2000. Somatic embryogenesis in Clematis integrifolia X C. viticella. Plant Cell Tissue and Organ Culture 62(2), 163-165.

Novak FJ, R Afza, M van Duren, M Perea-Dallos, BV Conger and T Xiaolang. 1989. Somatic embryogenesis and plant regeneration in suspension cultutes of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Biotechnology 7, 154-159.

Nikam TD and MG Shitole, 1999. In vitro culture of Safflower L. cv. Bhima: Initiation, growth optimization and organogenesis. Plant Cell Tissue and Organ Culture 55(1), 15-22.

Nasiruddin KM, R Begum and S Yasmin, 2003a. Protocorm like bodies and planlet regeneration from Dendrobium formosum leaf callus. Asian Journal of Plant Sciences 2(13), 955-957.

Nasiruddin KM, R Begum, S Yasmin, SK Talukder and L Hassan, 2003b. Shoot proliferation of Dendrobium orchid with BAP and NAA. Journal Biological Sciences 3(11), 1058-1062.

Qosim WA, R Purwanto, GA Wattimena dan Witjaksono. 2007. Pengaruh irradiasi sinar gamma terhadap kapasitas regenerasi kalus nodular tanaman manggis. Hayati Journal of Biosciences 14(4), 140-144.

Syabid, F dan U. Mariska, 1997. Pengaruh media dan zat pengatur tumbuh terhadap induksi dan regenerasi kalus jahe secara in vitro. Jurnal Littri 3(4), 145-150

Zhao F, W Wang, F Fo-Shcng, F Wu, Y Zhong-Qi and W Wan-Yung. 2007. High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall, ex Lindl. Plant Cell Tissue and Organ Culture 90(2), 131 - 139. ISSN 0167-6857.

http://www.anggrek.org/sekilas kulturiaringan aneerek.html -Generated 3 November 2008.

http://www.Doliteknikibr.itgo.com/P8.htm - Generated 3 November 2008.

http://en.wikiDedia.org/wiki/Clematis - Generated 3 November 2008.

DOI: https://doi.org/10.14203/beritabiologi.v9i3.790

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