KERUSAKAN DINDING SEL Escherichia coli OLEH MINYAK ATSIRI TEMU KUNCI (Kaempferiapandurata)

Miksusanti Miksusanti, Betty Sri Laksmi Jennie, Bambang Ponco, Gatot Trimulyadi
10.14203/beritabiologi.v9i1.775
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Abstract

Antibacterial activity of temu kunci (Kaempferiapandurata) essential oil against Escherichia coli Kl.l was analyzed. Activity of antibacterial essential oil was analyzed through it's ability to leak the Escherichia coli Kl.l cell wall and altering it.Minimum Inhibitory Concentration (MIC) of temu kunci essential oil is 0.11% (v/v). Further studies were conducted using the concentration of 1 MIC and 2 MIC.Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry(UV).Alteration of cell wall was analyzed with scanning electron microscopy (SEM).The optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm.The result showed that K. pandurata essential oil could leak the inorganic ion Ca 17-53%, and K* 9-43% from the bacteria and alter the cell wall of the bacteria.

Keywords

Escherichia coli Kl.l, temu kunci, Kaempferia pandurata, kebocoran ion, perubahan dinding sel, protein, asam nukleat.

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References

Andrews RE, LW Parks and KD Spence. 1980. Some effects of mono terpenes on certain microorganisms. Apll. Environ. Microbiol. 40, 301-304.

Brookes P, DJ Harris and RB Longmore. 1996. Isolation of minor lupin alkaloids. A simple procedure for the isolation of angustifoline from Lupinus angustifolius (cv. Fest) seeds, with application to other lupin alkaloids. J. Agric. FoodChem. 44 (8), 2129-2133.

Cosentino S, CIG Tuberoso, B Pisano, M Satta, V Masca, E Arzedi and F Palmas, 1999. In vitro antimicrobial activity and chemical composition of Sardinian Tymus essential oils. Letter in Applied Microbiology 29,130-135.

Corre J, JJ Lucchini, GM Mercier and A.Cremieux. 1990. Antibacterial activity of the phenethyl alcohol and resulting membrane alterations. Res. Microbiol. 141, 483-497.

Carson CF, JM Brian dan TV Riley. 2002. Mechanism of action of tea tree oil on Staphylococcus aureus determined by time-kill, lyses, leakage, and salt tolerance assays and electron microscopy. Antimicrobial Agent and Chemotherapy 6,1914-1920.

Cox SD, CM Mann, JL Markham, HC Bell, JE Gustafson, JR Warmington and Wyllie SG 2000. The mode of antibacterial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. of Appl. Microbiology 88, 170-175.

Delaquis P, J Stanich, K Girarad and G Mazza. 2002. Antimicrobial activity of individual and mixed fraction of dill, cilantro, coriander, and eucalyptus essential oils. International. J. Food Microbiology 74,101-109.

Fessenden RJ dan JS Fessenden. 1997. Kimia Organik, Edisi 3. Erlangga Jakarta Fardiaz S. 1992. Mikrobiologi Pengolahan Pangan Lanjut. PAU Pangan dan Gizi - Institut Pertanian Bogor.

Gilbert P. 1984. The Revival of Micro-organism Sublethally Injured by Chemical Inhibitors. In: The Revival of Injured Microbes. MJE Andrew and AD Russel (Eds). Academic Press. London.

Gammel CG and V Lorian. 1996. Effect of Low Concentration of Antibiotics on Bacterial. Ultrastructure, Virulence and Suscebtibility to Immunodefence: Clinical Significance. In: Antibiotics in Laboratory Medicine. V Lorians (Ed.). Fourth ed. Williams & Wilkins, London.

Ingram LO. 1981.Mechanisms of lysis of. E.coli by ethanol and other chaotropoc agents. J. Bacteriol. 146(1), 331-335.

McGuire MD and RS Conrad. 2000. Morphological and biochemical alteration in S.epidermis stepwise adapted to vancomycin resistance. Proceedings of the Oklahoma Academy of Science Vol. 80.

Madigan MT, JM Martinko and J Parker. 2003. Brook Biology of Microorganisms. Tenth Edition, Southern Illionis University, Carbondale.

Park SJ, HW Park and J Park. 2003. Inactivation kinetics of food poisoning microorganisms by carbon dioxide and high hydrostatic pressure. J. FoodSci. 68 (3), 976-981.

Rasooli I, MB Razaei and A Allameh. 2006. Ultratructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes. Int. Journal of Infectious Diseases 10,236-241.

Ruberto G, dan Baratta MT, 2000. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry 69, 167-174.

Russel AD. 1984. Potential Sites of Damage in Microorganisms Exposed to Chemical or Physical Agent. In: The Revival of Injured Microbes. MHE Andrew and AD Russel (Eds.). Academic Press. London.

Salehi P, A Saholi, F Eftekhar, S Nejad-Ebrahimi and M Yousefzadi. 2005. Essential oil composition, antibacterial and antioxidant activity of the oil and various extract of Ziziphora clinopodioides subsp. rigida (Boiss) Rech. from Iran. J. Agric Food Chem. 28, 52 -56.

Shi Bihong and Xuhua Xia. 2003. Morphological changes of Psedomonas psedoalcaligenes in response to temperature selection. Current Microbiology 46,120-123.

Sikkema J, AM Jan, Bont de, B Poolman. 1994. Interactions of cyclic hydrocarbons with biological membranes. Journal of Biological Chemistry 269,8022- 8028.

Skocibusic M, N Bezic and A Dunkic. 2006. Phytochemical composition and antimicrobial activities of essential oils from Satureja subspicata Vis. growing in Croatia. Food Chem. 96, 20-28.

Trombetta D, A Saija, G Bisignano, S Arena, S Caruso, G Mazzanti, N Ucella and F Castelli. 2005. Mechanisms of antibacterial action of three monoterpenes. Antimicrobial Agent and Chemoterapy, 49(6), 2474-2478.

Ultee A, MHJ Bennik and R Moezelaar. 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Applied and Environmental Microbiology 4,1561-1568.

Xianfei X, C Xiaoqiang, Z Shunying and Z Goulin. 2006. Chemical composition and cntimicrobial activity of essential oils of chaenomeles speciosa from China Food Chemistry2,15-19.

DOI: https://doi.org/10.14203/beritabiologi.v9i1.775

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