KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN)

Sumaryam Sumaryam, Kusyairi Kusyairi, Sri Oetami, Hari Suprapto, Garry Cores de Vries
| Abstract views: 441 | PDF views: 1381

Abstract

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.

Keywords

Chromileptes altivelis, Cell line, Viral Nervous Necrosis

Full Text:

PDF

References

Breuil G, JR Bonami, JF Pepin and Y Pichot. 1991. Viral infection (picorna-like virus) associated with mass mortalities in hatchery-reared sea-bass Dicentrarchus labrax larvae and juvenile. Aquaculture 97, 109-116.

Burleson FG, TM Chambers and DL Wiedbrauk. 1992. Virology: A Laboratory Manual, 25-32. Academic Press.

Fukuda Y, HD Nguyen, M Furuhashi and T Nakai. 1996. Mass mortality of sevenband grouper Epinephelus septemfasciatus, associated with viral nervous necrosis (VNN). Fish Pathology 31, 165-170.

Hasegawa S, T Somamoto, C Nakayasu, T Nakanishi and N Okamoto. 1997. A cell line (CFK) from fin of isogeneic Ginbuana Crussian carp. Fish Pathology 32, 127-128.

http://rn.antaranews.eom. 2011. Produksi Kerapu Indonesia Diperkirakan Geser China. Mori K, T Nakai , M Nagahara, K Muroga, T Mekuchi and T Kanno. 1991. A viral disease in hathery-reared larvae and juvenils of redspot grouper. Fish Pathology 26, 209-210.

Munday BL, JS Langdon, A Hyatt and D Humphrey. 1992. Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenil barramundi, Lates carcarifer Bloch. Aquaculture 103, 197-211.

Muhajir dkk. (2006). Pembuatan Kultur Sel Kerapu (Chromileptes altivelis) untuk Replikasi Viral Nervous Necrosis (VNN) dan Penyimpanan dengan Cryopreservasi. Direktorat Jendral Pendidikan Tinggi Departemen Pendidikan Nasional Indonesia, Jakarta.

Nagai T, T Nakatsugawa, T Nishizawa and K Muroga. 1998. Primary culture of hemocyte from Japanese Black Abalone Nordotis discus discus. Fish Pathology 33, 147-148.

Silverstein AM. 1989. A History Of Immunology. Academic Press Inc. Harcout Brace Jovanovich, Publisher. New York.

Tanaka S, K Mori, M Arimoto and T Nakai. 1998. Protective immunity of sevenband grouper, Epinephelus septemfasciatus Thunberg, against experimental viral nervous necrosis. Journal of Fish Diseases 24, 75-85.

Wolf K and MC Quimby. 1962. Establisment of eurythermic line of fish cell line in vitro. Science 135, 1065-1066.


Refbacks

  • There are currently no refbacks.