Apriadi Situmorang, Anky Zannati, Dwi Widyajayantie, Satya Nugroho
| Abstract views: 318 | PDF views: 354


Growth and biochemical characters of thirty-three Nipponbare-based rice mutant lines independently harboring activation-tag in salt stress condition were investigated. Rice cv Nipponbare wild type, IR29 and Pokkali were used as isogenic, susceptible, and tolerant cultivars, respectively. Plants were grown in Yoshida nutrient solution containing NaCI (6 g/1) as the stress treatment and in Yoshida nutrient solution without NaCI as the control. Evaluation of salt tolerance was conducted at 21 days after salinization.The results showed that there was a significant difference in growth characters among genotypes in reduction of the plant height,root length, shoot fresh weight, root fresh weight, shoot dry weight, as well as in the root dry weight. Biochemical characters of the genotypes also showed significant difference in their reduction of chlorophyll-a and chlorophyll-b concentrations,total carotenoids and proline accumulation. Based on multivariate growth and biochemical characters,T3.PMO.VI.63.5a.30.9,T3.PMO.VI.30.1a.21.1, T3.PMO.V1.63.5a.33.7F3.PUR.IX.49.1d.l.6.5,T3.PMO.VI.81.3a.4.4, F3.PUR.IX.49.1d.l.6.5,and F3.PUR.VIII.5.1f.l.4.8 were assumed as potential salt-tolerant lines, respectively, while mutant lines T3.PMO.VI.30.1a.l07.7,T3.PMO.VI.63.5a.20.6,T3.PMO.III.4.4c.7.2,andT3.PMO.VI.30.1a.51.1 were assumed as salt-susceptible lines respectively.


Rice, salt tolerance, salt sensitive, insertional mutant, growth characters, biochemical characters.

Full Text:



Ashraf MM and R Foolad 2007. Role of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experiment Botany 59, 206-216.

Bates LS, RP Warden and ID Teare 1973. Rapid determination of free proline for water stress studies. Plant Soil 39, 205-207. Borsani O, V Valvuesta and MA.

Botella 2003. Developing salt tolerant plants in a new century: a molecular biology approach. Plant Cell, Tissue and Organ Culture 73, 101-115

Bremont JFJ, AB Flora, EH Lucero, MR Kessler, JAA Gallegos and JGR Pimentel 2006. Proline accumulation in two bean cultivars under salt stress and the effect of polyamines and ornithine. Biologia Plantarum 50(4), 763-766

Cha-um S and C Kirdmanee 2009. Proline accumulation, photosynthetic abilities and growth characters of sugarcane (Saccharum officinarum L.) plantlets in response to iso-osmotic salt and water-deficit stress. Agricultural Science China 8(1), 51-58.

Cha-um S, T Trakulyingcharoen, P Smitamana and C Kirdmanee. 2009. Salt tolerance in two rice cultivars differing salt tolerant abilities in responses to isoosmotic stress. Australian Journal of Crop Science 3(4), 221-230

Cha-um S, M Ashraf and C Kirdmanee 2010. Screening upland rice (Oryza sativa L. ssp. indica) genotypes for salt-tolerance using multivariate cluster analysis. African Journal of Biotechnology 9(30), 4731-4740

FAO (Food and Agriculture Organization). 2008. FAO Land and Plant Nutrition Management Service, http:/ ag/agl/agll/spush (accessed: May 11, 2009).

Flowers TJ. 2004. Improving crop salt tolerance. Journal of Experimental Botany 55, 307-319.

Gregorio GB, O Senadhira and RD Mendoza 1997. IRRI Discussion Paper Series No. 22. Havaux M, F Tardy and Y Lemoine 1998. Photosynthetic light-harvesting function of carotenoids in higherplant leaves exposed to high light irradiances. Planta 205, 242-250.

Hiei Y, S Otha, T Komari and T Kumashiro 1994. Efficient transformation of rice (Oryza sativa L) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6(0), 001-011

IRRI (International Rice Research Institute). 2010. Salttolerance Rice, (Accessed: 15 Des 2010). Khush GS. 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Molecular Biology 59, 1-6.

Krlshnan A, E Guiderdoni, G An, YC Hsing, C Han, MC Lee, SM Yu, N llpadhygya, S Ramachandran, Q Zhang, V Sundareian, H Hlrochlka, H Leungand A Perelra. 2009. Mutant Resources in Rice for Functional Oenomics of the Grasses, Plant Physiology 149, 165-170

Lichtenthaler HK. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymology 148, 350-380.

Munns R and M Tester 2008. Mechanism and salinity tolerance. Annual Review of Plant Biology 59, 651-681.

Nugroho S, KR Trijatmiko, S Rachmawati, A Zannati dan S Purwantomo. 2006. Upaya pengembangan populasi mutagenic lines padi pembawa activation tag dengan transposon Ac/Ds melalui transformasi dengan Agrobacterium. Prosiding Seminar Nasional Bioteknologi, 15-16 November 2006, 236-242.

Satarugsa P, W Youngmee and S Meesawat. 2006. The three-year mapping and monitoring of underground cavity expansion with 2D resistivity survey: what has revealed? In: Wang, J., Xu, Y., Xia, J., and Chen, C.(Eds.), Proceeding of the 2nd International Conference on Environmental and Engineering Geophysics (1CEEG 2006), 4-9 June 2006, 265-269. Wuhan, China.

Shabala SN, SI Shabala, AI Martynenko, O Babourina and IA Newman 1998. Salinity effect on bioelectric activity, growth, Na+ accumulation and chlorophyll fluorescence of maize leaves: a comparative survrand prospects for screening. Australian Journal Plant Physiology 25, 609-616.

Situmorang A, A Zannati, D Widyajayantie dan Nugroho. 2010. Seleksi genotipe padi mutan inse toleran cekaman salinitas berdasarkan karakiepertumbuhan dan biokimia. Jurnal Agroncn. Indonesia 38(1), 8-14

Soussi M, A Ocana and C Lluch 1998. Effects of salt stres on growth, photosynthesis and nitrogen fixation T chick-pea (deer arietinum L.). Journal : Experimental Botany 49, 1329-1337.

Szabolcs I. 1989. Salt-Affected Soils. Boca Raton, FL: CRI Press. USDA-ARS. 2008. Research Databases. Bibliography on Sc Tolerance. George E.Brown, Jr. Salinity Lab. US Der Agric, Agric. Res. Serv. Riverside, CA. http (accessed: May 11, 2009)

Yoshida S, DA Forno, JH Cock and KA Gomez 19 Laboratory Manual for Physiological Studies of Rio International Rice Research Institute, Manila.

Zeng L, SM Lesch and CM Grieve 2003. Rice growth yield respond to changes in water depth and salinir stress. Agricultural Water Management 59, 67-75


  • There are currently no refbacks.