IDENTIFIKASI 26 ISOLAT BAKTERI ENDOFITIK DAN FILOSFER PADI DENGAN ANALISIS SEKUEN16S RDNA

Nurul Hidayatun, Dwi N Susilowati, K Mulya K Mulya
| Abstract views: 491 | PDF views: 1713

Abstract

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.

Keywords

BLAST, endophytic bacteria, 16S rDNA, sequence

Full Text:

PDF

References

Di Fiori S and M Del Gallo. 1995. Endophytic bacteria: their possible role in the host plant. In: Azospirillum VI and Related Microorganisms. I Fendrik, M Del Gallo, J Vanderleyden and M de Zamaroczy (Eds.). Springer-Verlag, Berlin.

Drancoutr M, C Boiler, A Carlioz, R Martelin, JP Gayral and D Raoult. 2000. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. Journal of Clinical Microbiology 38(10), 3623-30.

Gardner JM, AW Feldman and DH Stamper. 1983. Role and fate of bacteria in vascular occlusion of citrus. Physiological Plant Pathology 23, 295-303.

Gyaneshwar P, EK James, N Mathan, PM Reddy, B Reinbold-Hurek and J Ladha. 2001. Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens. Journal of Bacteriology. 183, 2634-2645.

Hallman J, AQ Hallman, WF Mahaffee and JW Kloeper. 1997. Bacterialendophytes in agricultural crops. Canadian Journal of Microbiology 43(10), 895-914.

Hoflich G, W Wiehe and G Kihn. 1994 Plant growth stimulation by inoculation with symbiotic and associative rhizosphere mechanisms. Experientia 50, 897-905.

Huang JS. 1986. Ultrastructure of bacterial penetration in plants. Annual Review of Phytopatology 24, 141- 57. Kloepper JW, EM Tipping and R Lifshitz. 1991. Plant growth promotion mediated by bacteria] rhizosphere colonizers. In: The Rhizosphere and Plant Growth, 315-326. DL Keister and PB Cregan (Eds.). Kluwer Academic Publishers, Dordrecht, The Netherlands.

Lazo GR, R Roffey and DW Gabriel. 1987. Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris. Phytopathology 77, 448-453.

Lesmana OS, HM Toha dan I Las. 2003. Deskripsi Varietas Unggul Baru Padi. Balai Penelitian Tanaman Padi, Sukamandi.

Mano H and H Morisaki. 2008. Minireview: Endophytic bacteria in the rice plant. Microbes and Environments 23, 109-117.

Mano H, F Tanaka, C Nakamura, H Kaga and H Morisaki. 2007. Cultivable endophytic bacterial flora of the maturing leaves and roots of rice plants (Oryza saliva) cultivated in a paddy field. Microbes and Environments 22, 175-185.

Mulya K, DN Susilowati, A Akhdiya, I Manzila, H Purwanti, RD Hastuti, D Wahyuno, D Manohara, H Kurniawan, N. Azizah, S Soedjono dan Simanungkalit. 2004. Konservasi dan Karakterisasi Genetik Mikroba Pertanian. Laporan Hasil Penelitian. Balai Besar penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor.

Rodiyah. 2003. Distribusi dan Diversitas Genetik Bakteri Diazotrof Endofitik pada Tebu (Saccharum qfficinarum L). Tesis. Program Pasca Sarjana UGM, Yogyakarta.

Soendoro D. 2010. Dynammic programming dalam menentukan arti urutan untaian gen IF3051 strategi algoritmik Tahun 2009/2010. Makalah. Program Studi Teknik Informatika, Sekolah Teknik Elektro dan Informatika, Institut Teknologi Bandung.

Someya N, M Nakajima, T Hibi, I Yamaguci and K Akutsu. 2002. Induced resistance to rice blast by antagonistic bacterium Serratia marcescens Strain B2. General Plant Pathology 68, 177-182.

Strobel G and B Daisy. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67 (4), 491-502.

Susilowati DN, K Mulya, N Azizah, H Purwanti, A Suhendar, I Manzila, RD Hastuti, D Wahyuno, D Manohara, N Hidayatun, S Salma, Nurichana, S Soedjono, R Saraswati dan K Herlina. 2005. Konservasi dan Karakterisasi Mikroba Pertanian. Laporan Hasil Penelitian. Balai Besar penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor.

Susilowati DN, A Akhdiya, K Mulya, E Pratiwi, H Purwanti, A Suhendar, I Manzila, RD Hastuti, D Wahyuno, D Manohara, N Hidayatun, S Salma, Nurichana, S Soedjono, R Saraswati dan K Herlina. 2006. Konservasi dan Karakterisasi Mikroba Pertanian. Laporan Hasil Penelitian. Balai Besar penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor.

Van Peer R, HLM Punte, LA De Wega and B Schippers. 1990. Characterization of root surface and endorhizosphere Pseudomonads in relation to their colonization of roots. Applied and Environmental Microbiology 56, 2462-2470.

Weidner S, W Arnold and A Pukle. 1996. Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by retriction fragmenth length polymorphim analysis of gen encoding 16S rRNA and 23S rRNA intergenic spacers, repetitive extragenic palindromic PCR genomic finger printing and partial 16S rRNA sequencing. Applied and Environmental Microbiology 64, 2096-2104.

Wiesburg WG, SM Barns, DA Pelletier and DJ Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of bacteriology, 173(2), 697-703.


Refbacks

  • There are currently no refbacks.