ELISITASI PENINGKATAN PRODUKSI AJMALISIN OLEH KALUS Catharantus roseus (L.) G. Don.

Marina Silalahi
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Abstract

An experiment to study the effect of soft fraction derived from Saccharomyces cerevisiae (Hansen) to elicit ajmalicine production by Catharanthus roseus (L.) G. Don. callus cultures has been conducted. Callus were induced from leaf segment and grew on Zenk medium supplemented with 2,5 x l0M NAA dan 10 M BAP respectively. Callus on the third subculture level were elicited with soft fraction derived from S. cerevisiae. The following concentrations of elicitor tested were 0, 0,5, 1,0 and 2,5 % (g/v), and the harvesting times were 0, 18, 36 and 72 hour respectively. Influence of elicitor to production of ajmalicine by the callus were analyzed qualitatively and quantitatively by using high performance liquid chromatography (HPLC). A significant increasing of ajmalicine content in the callus (303,475 ± 5,602 ug/gDW) was achieved by addition of elicitor of 0,5 % (g/v) after 36 hour. This study showed a significant increase of ajmalicine production in C. roseus callus cultures after being challenged with elicitor derived from S. cerevisiae i.e. 69,334%.

Keywords

Ajmalisin/ ajmalisine, elisitor/ elicitor, Catharantus roseus, kalus/callus.

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References

Alexandrova R, I Alexandrova, M Velcheva and T Varadinova. 2000. Phytoproduct and Cancer. Exp. Pathol. Parasitol. 4, 15-26.

Aprianita. 1999. Pengaruh Pemberian Homogenat Jamur Pythium aphanidermatum (Edson) Fitzp.Terhadap kandungan Ajmalisin dalam Kultur Kalus Berakar C. roseus (L) G. Don. Tesis Magister. Jurusan Biologi. Institut Teknologi Bandung.

Asada M and ML Shuler. 1989. Stimulation of ajmalicine produetioan and excretion from Catharanthus roseus: effects of adsobtion in situ, elicitor and alginate immobilization. Applied of Microbiology Biotechnology 30, 475-481.

Buiteilaar RM, MT Cesario and J Trampler. 1991. Elicitation of thiopene production by root Tagetas patula. In: RM Buitelaar (Ed.)Production and Secretion of Secondary Metabolites by Plant Cell Cultures of Tagetas, 119-133. Wageningen.

Dos Santos RI. J Schripsema and R Verpoorte. 1994. Ajmalicine metabolism in Catharanthus roseus cell cultures. Phytochemistry 15(3), 667-681.

Eilert U, F Constabel and WGW Kurz. 1986. Elicitor stimulation of monoterpene indol alkaloid formation in suspensi cultures of Catharanthus roseus. Journal of Plant Physiology 126, 11-22.

Endress R. 1994. Plant Cell Biotechnology, 121-142. Spiringer-Verlag Berlin Heidelberg New York London Paris Tokyo Hongkong Barcelona Budapest.

Fitriani A. 1998. Pengaruh Pemberian Homogenat Jamur Pythium aphanidermatum (Edson) Fitzp. Terhadap Kandungan Ajmalisin Dalam Kultur Kalus Catharanthus roseus (L.) G. Don. Tesis Pasca-Sarjana. ITB. Bandung.

Funk C, K Gugler and P Brodelius. 1986. Increased secondary product formation in plant suspension cultures after treatment with a yeast carbohydrate preparation (elicitor). Phytochemistry 26(2), 401-405.

Mantell SH, and H Smith. 1983. Culturel Factor That Influence Secondary Metabolite Accumulation in Plant Cell and Tissue. In: SH Mantell and H Smith (Eds). Plant Biotechnology, 75-108. Cambrige University Press. New York.

Moreno PRH, R van der Heijden and R Verpoorte. 1994. Catharanthus roseus (L.) G. Don. Cell suspension cultures: a literature survey, updating from 1988-1993. Plant Cell Report 12, 702-705.

Morris P. 1986. Regulation of product synthesis in cell cultures of Catharanthus roseus. III. Alkaloid metabolism in cultured leaf tissue and primary callus. Planta Medica 12, 123-132.

Pandiangan D. 2006. Respon pertumbuhan kalus Catharanthus roseus yang diberi perlakuan triptofan. biotika 5(2), 48-56.

Pieron S, P Boxus and D Dekegel. 1998. Hystological sudy of nodule morphogenesis from Cinchorium intybus L. leaves cultivated in vitro. In Vitro Cell. Dev. Biol. Plant 34, 87-93.

Peltonen S, L Mannonen and R Karjalainen. 1997. Elicitor-induced changes of phenylalanine ammonialyase activity in barley cell suspension cultures. Plant Cell and Organ Culture 50, 185-193.

Rudge K and P Morris. 1986. The effect of stress osmotic on growth and alkaloid accumulation in Catharanthus roseus. In: Secondary Metabolism in Plant Cell Cultures, 85-191. P Morris, AH Scragg, A Stanfford and MW Fower. Cambridge University Press.

Senoussi MM, J Creche and M Rideau. 2007. Relation between hypoxia and alkaloid accumulation in Catharanthus roseus cell suspension Journal of Applied Sciences Research 3(4), 287-290.

Sim SJ, HN Chang and JR Liu. 1994. Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus: effects of in situ adsorption, fungal elicitation and permiabilization. Journal of Fermentation and Bioengineering 78(3), 229-234.

Suvarnalatha G, L Rajendran, GA Ravishankar and LV Venkataraman. 1994. Elicitation of anthocyanin production in cell cultures of carrot (Daucus carota L.) by using elicitor an abiotik stress. Biotechnology Letters 16(12), 1275-1280.

Verpoorte R, R Van der Heijden, WM Van Gulik and HJG Ten Hoopen. 1991. Plant Biotechnology for Production of Alkaloid : Present Status and Prospects. 40, 109-153. Academic Press. Inc. New York.

Wiendi NMA, GA Wattimena dan LW Gunawan. 1992. Produksi Metabolit Sekunder dengan Kultur Jaringan. Dalam: Bioteknologi Tanaman. GA Wattimena (Ed.), 168-219. Depdikbud. Direktorat Pendidikan Tinggi. Pusat Antar Universitas. IPB. Bogor.

Yeoman MM and PA Aitchinson. 1973. Growth Pattern in Tissue (Callus). In: Plant Tissue and Cell Culture, 240-268. Blackwell Sci. Publ. Oxford.

Zbao J, W Zhu, Q Hu, and Y Guo. 2001. Compact callus cluster suspension cultures of Catharanthus roseus with enhanced indole alkoaloid biosinthesis J. In Vitro Cellular & Developmental Biology Plant 37(1), 68-72.


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