ANALISIS KERAGAMAN GENETIK KENTANG HITAM

Kusumadewi Sri Yulita, Fajarudin Ahmad, Diyah Martanti, Yuyu S. Poerba, Herlina Herlina
10.14203/beritabiologi.v13i2.686
| Abstract views: 658 | PDF views: 1160

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.

Keywords

Kentang hitam, genetic variation, ISSR, RAPD, Plectranthus rotundifolius (Poiret) Sprengel.

Full Text:

PDF

References

Campbell BCS, G LeMare, Piperidis and ID Godwin. 2011. IRAP, a retrotransposon-based marker system for the detection of somaclonal variation in barley. Molecular breeding 27(2), 193-206.

Doyle JJ and JL Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15.

Fracaro F and S Echeverrigaray. 2006. Genetic variability in Hesperozygis ringens Benth. (Lamiaceae), an endangered aromatic and medicinal plant of Southern Brazil. Biochemical genetics 44, (11/12) DOI:10.1007/s10528-006-9044-z.

Isshiki S, N Iwata and MMR Khan. 2008. ISSR variation in eggplant (Solanum melongena L.) and related Solanum species. Scientia Horticulturae 117, 186-190.

Jain PK, L Saini, MH Pathak and VK Gupta. 2007. Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs). African Journal of Biotechnology 6 (17), 1987-1989.

Jansen PCM. 1996. Plectranthus rotundifolius (Poiret) Sprengel. In: Plant Yielding non-seed Carbohydrates. Flach M and F Rumawas (Eds.), 9: 156-159.

Prosea Foundation. Bogor. Jiminez JF, P Sanchez-Gomez, J Guemes, O Werner and JA Rossello. 2002. Genetic variability in a narrow endemic snapdragon (Antirrhinum subaeticum, Schrophulariaceae) using RAPD markers. Heredity 89(5), 387-393.

Liu J, L Wang, Y Geng, Q Wang, L Luo and Y Zhong. 2006. Genetic diversity and population structure of Lamiophlomis rotate (Lamiaceae), an endemic species of Qianghai-Tibet Plateau. Genetica 28, 385-394.

Lukhoba CW, MSJ Simmonds and AJ Paton. 2006. Plectranthus: A review of ethnobotanical uses. Journal of Ethnopharmacology 103(1), 1-24. http:// www.sciencedirect.com/science/article/pii/ (diunduh 18 april 2013) S0378874105006215 (diunduh pada tanggal 12 Januari 2011).

Malik SK, R Chaudhury, OP Dhariwal and RK. Kalia 2006. Collection and characterisation of Citrus indica Tanaka and C. macroptera Montr.: wild endangered species of north eastern India. Genetic resources and crop evolution 53, 1485-1493.

Mattioni C, M Casasoli, M Gonzales, and R Ipinza. 2002. Comparison of ISSR and RAPD markers to characterise three Chilean Notofagus species. Theoritical and Applied Genetics 104, 1064-1070. Plectranthus rotundifolius (Poir.) Spreng. www.theplantlist.org (diunduh 12 januari 2014).

Poerba YS, A Wawo and KS Yulita. 2007. Keragaman Fenotipe RAPD Santalum album L. di Pulau Timor bagian timur. Berita Biologi 8(6), 537- 546.

Rohlf FJ. 1998. NTSYS-PC. Numerical Taxonomy and Multivariate Analysis. Version 2.02i. New York: Exeter Software.

Rucinska A and J Pulchaski. 2010. Comparative molecular studies on the genetic diversity of an ex situ garden collections and its source population of the critically endangered polish endemic plant Cochlearia polonica E. Frochlich. Biodiversity Conservation DOI: 10.1007/s10531-010-9965-z.

Tingey SV, JA Rafalski and MK Hanafey. 1994. Genetic analysis with RAPD markers. In: Plant Molecular Biology. Coruzzi C, Puidormenech P (eds), 491-498.Berlin, Pringer.

Shaaban EA, SKH Abd-El-Aal., NS Zaied and AA Rizkalla. 2006. Assessment of genetic variability on some orange accessions using RAPD DNA markers. Research Journal of Agriculture and Biological Sciences 2(6), 564-570.

Siregar IZ, T Yunanto and P Pamoengkas. 2008. Implikasi genetic metode pembiakan tanaman Shorea johorensis Foxw. Pada sistem silvikultur tebang pilih tanam jalur (TPTJ). Biodiversitas 9(4), 250-254.

Williams JGK, AR Kubelik, KJ Livak, JA Rafalski and SV Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531-6535.

Yadav PV, KU Suprasanna, Gopalrao, and BV Anant. 2006. Molecular profiling using RAPD technique of salt and drought tolerant regenerants of sugarcae. Sugar Technology Reviews 8(1), 63-68.

Zietkiewicz EA Rafalski and D Labuda. 1994. Genomic fingerprinting by simple sequence repeat (SSR)- anchored polymerase chain reaction amplification. Genomics 20, 176-183.

DOI: https://doi.org/10.14203/beritabiologi.v13i2.686

Refbacks

  • There are currently no refbacks.