ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS

Rudhy Gustiano, Tia Oktaviani, Dinar Tri Soelistyowati, Irin Iriana Kusmini, Wahyutomo Wahyutomo, Gleni Hasan Huwoyon
| Abstract views: 506 | PDF views: 595

Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.

Keywords

RAPD, morphometric, snakehead fish, Channa

Full Text:

PDF

References

Allendorf FW and FM Utter. 1979. Population Genetics. In: Fish Physiology 8: Bioenergetics and Growth.WS Hoar, DJ Randall and JR Brett (Eds),407–454. Academic Press, NY.

Ambak MA, MAB Abol, I Patimah and MT Bui. 2006. Genetic variation of snakehead fish (Channa striata) population using random amplified polymorphic DNA. Biotechnol. 5, 104-110.

Bijaksana U. 2010. Kajian fisiologi reproduksi ikan gabus, Channa striata Blkr di dalam wadah dan perairan rawa sebagai upaya domestikasi. Disertasi. Sekolah Pascasarjana- Institut Pertanian Bogor.

Bookstein FL, B Chernoff, R Elder, J Humphries, G Smith and R. Strauss. 1985. Morphometric in Evolutiany Biology. Academy of Natural Sciences of Philadelphia 15, 1-277.

Dunham RA. 2011. Aquaculture and Fisheries Biotechnology: Genetic Approach. CAB International, 456. Wellingford, UK.

Falconer FS and TFC MacKay. 1996. Introduction to Quantitative Genetics, 464. Longman, England.

Gjedrem T. 2005. Selection and Breeding Program in Aquaculture, 364. Akvaforsk, As, Norway.

Gustiano R and L Pouyaud. 2005. Phenetic analysis of 28 species pangasiid catfishes from Asia.

Zuriat 16, 66-72.

Hallerman EM. 2003. Population Genetics: Principles and Applications for Fisheries Scientist, 458. American Fisheries Society.University of California, USA

Hassanien HA, E Mohumad, O Ali and I Hania. 2004. Genetic diversity of Nile tilapia population revealed by randomly amplified polymorphic DNA (RAPD). Aquacult. Res. 35, 587-593.

Kapuscinski AR and LD Jacobson. 1987. Genetic guide lines for fisheries management. University of Minnesota, Minnesota Sea Grant College Program, Sea Grant Research Report 17, 1- 66.

Kirpichnikov VS. 1981. Genetic Bases of Fish Selection, 410. Springer Verlag, Berlin. Miler P. 1997. Tools for population genetic analyses (TFPGA): A window program for the analysis of allozymes and molecular population genetic data, 30. Northern California University. USA.

Shafri MA and M Abdul. 2012. Therapeutic potential of haruan (Channa striata): from food to medicinal uses. Mal. J. Nutr. 18, 125-136.

Strauss RE and FL Bookstein. 1982. The truss: body form reconstructions in morphometrics. Syst. Zool. 31, 113-135.

Tave D. 1993. Genetics for Fish Hatchery Managers, 415. Kluwer Acad. Publ. Netherland.


Refbacks

  • There are currently no refbacks.