KEMAMPUAN BEBERAPA JAMUR TANAH DALAM MENGURAIKAN PESTISIDA DELTAMETRIN DAN SENYAWA LIGNOSELULOSA

YB. Subowo
10.14203/beritabiologi.v12i2.537
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Abstract

Some of soil fungi capable in degrading pesticide deltamethrin, therefore they can be used as fertilizer in organic farming. As a biofertilizer ,fungus also must be able to provide nutrients for plants. The purpose of the study was to obtain fungal isolates that have the ability to decompose pesticides deltamethrin and lignocellulose compounds, dissolved inorganic phosphate compounds and produce growth hormone IAA (Indole Acetic Acid) . The fungal isolates will then be used in the manufacture of bio-fertilizers. Soil samples were put into mineral medium containing deltamethrin, fungus that grew on this medium would then be isolated. The ability of fungus in decomposing deltamethrin was observed using GC. While the ability of fungi to decompose lignocellulose, to dissolve inorganic phosphate compounds and to produce IAA was observed using a spectrophotometer. The results showed that the fungus Aspergillus niger TR1 had the ability to degrade 90.68% of deltamethrin 500 ppm after 1 h incubation and to degrade 0.46% of Poly R - 478 by after 30 min incubation. In addition, this fungus had cellulase activity of 0.029 units/ml and were able to dissolve inorganic phosphate compounds and produce IAA.

Keywords

soil fungi, deltamethrin degradation, Aspergillus niger, biofertilizer

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References

Barroso CB, GT Pereira, E Nahas. 2006. Solubilization of Ca-HPO4 and AlPO4 by Aspergillus niger in culture media with different carbon and nitrogen sources. Brazilian Journal of Microbiology 37, 434-438.

Bhanu S, S Archana, K Ajay, JL Bhatt, SP Bajpai, PS Singh, B Vandana. 2011. Impact of deltamethrin on enviroment, use as an insecticide and its bacterial degradation-a preliminary study. International Journal of Environmental Sciences 1(5): 977-980.

Bilkay IS, S Karakoc, N Aksoz. 2010. Indole-3-acetic acid and gibberellic acid production in Aspergillus niger. Turk J. Biol, 34: 313-318.

Chen S, K Lai, Y Li, M Hu, Y Zhang, Y Zeng. 2011. Biodegradation of deltamethrin and its hydrolisys product 3-phenoxybenzaldehyde by a newly isolated Streptomyces aureus strain HP-S-01. Appl. Microbiol. Biotechnol. 90: 1471-1483.

Dietz S, MD Roman, SL Birkel, CH Maus, P Neumann, and R Fischer. 2009. Ecotoxicological and environmental profile of the insecticide deltamethrin. Bayer Crop Science Journal 62: 211-226.

Garraway MO and RC Evans. 1991. Fungal Nutrition and Physiology.Krieger publishing Company. Malabar, Florida.p: 231.

Hasan HAH. 1999. Fungal utilization of organophosphate pesticides and their degradation by Aspergillus flavus and A. sydowii in soil. Folia Microbiol. 44(1): 77-84.

Johnson M, B Luukinen, K Buhl, D Stone. 2010. Deltamethrin Technical Faact Sheet. National Pesticide Information Center, Oregon State University Extention Services. http://www.npic.orst.edu/factsheets/Delta tech.pdf

Khan SU, L Zhang and MH Akhtar. 1984. Investigated the formation of bound residu of deltamethrin. J. Agric. Food Chem. 32: 1141-1144.

Kotterman MJJ, RA Wasseveld and JA Field. 1996. Hydrogen Peroxide Production as a limiting factor in xenobiotic compound oxidation by Nitrogen –Sufficient cultures of Bjerkandera sp strain BOS55 overproducing peroxidases. Appl. Environ. Microbiol 62(3): 880-885.

Liu YH, YC Chung, and Y Xiong. 2001. Purification and Characterization of a Dimethoate-Degrading Enzyme of Aspergillus niger ZHY256, Isolated from Sewage. Appl. Environ. Microbiol. 67(8): 3746–3749.

Maloney SE .2001. Pesticide degradation In Fungi in Bioremediation by GM Gadd (ed). Cambridge Universiry Press, 497 pp.

Matsumura F. 1985. Toxicology of Insecticides. Vol 2. Plenum Press, New York. Meyer O and HG Schlegel. 1983. Biology of aerobic carbon monoxide oxidizing bacteria. Ann. Rev. Microbiol. 37: 277-310.

Miller GL. 1959. Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry,31 (3): 426-429.

Nare RWA, PW Savadogo, Z Gnankambary and MP Sedogo. 2010. Effect of endosulfan, deltametrin and profenofos on soil microbial respiration characteristics in two land uses systems in Burkina Faso. Research Journal of Environmental Sciences 4(3): 261-270.

Parr JF, SB Hornick and RI Papendick. 2002. Transition from conventional agriculture to natural farming system: The role of microbial inoculants and biofertilizer.http://www.emtech.org/data/pdf/0103.pdf.

Pfennig N. 1974. Rhodopseudomonas globiformis sp n. a new species of the Rhodospirillaceae. Arch. Microbiol. 100: 197-206.

Ruzo LO and JE Casida. 1979. Degradation of Deltamethrin on cotton plants. J. Agric. Food Chem. 27: 572-575.

Subowo YB. 2012. Seleksi jamur tanah pendegradasi selulosa dan pestisida deltametrin dari beberapa lingkungan di Kalimantan Barat. Jurnal Teknologi Lingkungan, 13(2): 221-230.

Watanabe FS and SR Olsen. 1965. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Sci. Soc. American Proceed. 29:677-678.

Yadav J, JP Verma and KN Tiwari. 2011. Plant growth promoting activities of fungi and their effect on chickpea plant growth. Asian Journal of Biological Sciences, 4 (3): 291-299.

DOI: https://doi.org/10.14203/beritabiologi.v12i2.537

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