Joko Prasetiyono, Tasliah Tasliah, Ahmad Dadang, Fatimah Fatimah
| Abstract views: 638 | PDF views: 731


The improvement of rice (Oryza sativa L.) Ciherang variety for early maturity and high production traits was carried out by Marker Assisted Backcrossing (MAB) method using Nipponbare as donor parent. The foreground selection of Hd2 gene was laid on flanking markers of RM1362 and RM7601 in QTL region. The selection process of F1 to BC2F2 plants were based on molecular markers and agronomic characters. The BC2F3 plants were challenged to bacterial leaf blight to know their resistance in this hybridization. The results indicated that the foreground and background selection were not sufficient as selection tools therefore they would be more accurate if assosiated with agronomic characters. Four selected lines derived from Ciherang x Nipponbare crossed (BC2F3 plant # 283, 307, 373, and 462) could be promising lines with early maturity and high productioncompared to Ciherang. Selected BC2F3 lines flowered earlier than original Ciherang up to 7-10 days, while the yield increasing was 3.55 to 9.2% higher based on weight of filled grains/plant, and from 3.58 to 19.39% higher based on the number of filled grain/plant. However, all of BC2F3 lines were not resistant to bacterial leaf blight attack.


Rice, Oryza sativa L., MAB, Hd2, early maturity, high production

Full Text:



Badan Litbang Pertanian. 2009. Pedoman Umum IP Padi 400., [13 Desember 2010].

Berloo RV. 2008. GGT 2.0: Versatile software for vizualization and analysis of genetic data. Journal of Heredity 99(2), 232-236.

Dellaporta SL, J Wood, and JB Hicks. 1983. A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1(4), 19-21.

Direktorat Perlindungan Tanaman Pangan. 2011. Prakiraan serangan BLB pada padi di Indonesia masa tanam 2011. http: www., [15 November 2011].

Fujino K and H Sekiguchi. 2005. Mapping of QTLs conferring extremely early heading in rice (Oryza sativa L.). Theoritical dan Applied Genetics 111, 393-398.

Fujino K and H Sekiguchi. 2008. Mapping of quantitative trait loci controlling heading date among rice cultivars in the northernmost region for Japan. Breeding Science 58, 367-373.

IRRI. 1996. Standard Evaluation System for Rice, 52. Philipinnes. Jihai Z and S Zaongtan. 1988. Compatibility and heterosis between indica and japonica rice. Journal of Rice Science 2(1), 23-28.

Nonoue Y, K Fujino, Y Hirayama, U Yamanouchi, SY Lin and M Yano. 2008. Detection of quantitative trait loci controlling extremely early heading in rice. Theoritical and Applied Genetics 116, 715-722.

Prasetiyono J, T Suhartini, IH Soemantri, Tasliah, S Moeljopawiro, H Aswidinnoor, D Sopandie dan M Bustamam. 2012. Evaluasi beberapa galur-Pup1 tanaman padi (Oryza sativa L.) pada larutan hara dan lapangan. Jurnal Agronomi Indonesia 40 (2), 83-90.

Ribaut JM and M Hoisington. 1998. Marker-assisted selection : new tools and strategies. Trends in Plant Sci 3, 236-239.

Septiningsih EM, AM Pamplona, DI Sanchez, CN Neeraja, GV Vergara, S Heuer, AM Ismail and DJ Mackill. 2009. Development of submergence-tolerant rice cultivars: the SubI locus and beyond. Annals of Botany 103, 151-160.

Tasliah, J Prasetiyono, A Dadang, M Bustamam dan S Moeljopawiro. 2011. Studi agronomis dan molekuler padi umur genjah dan sedang. Berita Biologi 10(5), 663-673.

Vaithiyalingan M and N Nadarajan. 2010. Heterosis for yield contributing characters in inter sub-specific crosses of rice. Journal of Plant Breeding 1(3), 305-310.

Yamamoto T, Y Kuboki, SY Lin, T Sasaki and M Yano. 1998. Fine mapping of quantitative trait loci Hd-1, Hd-2, and Hd-3, controlling heading date of rice, as single mendelian factors. Theoritical and Applied Genetics 97, 37-44.

Yano M, H Yoshiaki, Y Kuboki, SY Lin, Y Nagamura, N Kurata, T Sasaki and Y Minoba. 1996. QTL analysis as an aid to tagging genes that control heading time in rice. Proceedings of the Third International Rice Genetics Symposium, 650-656. Manila 16-20 Oct 1995. GS Khush, G Hettel and T Rola (Eds.). International Rice Research Institute.

Yano M, S Kojima, Y Takahashi, H Lin and T Sasaki. 2001. Genetic control of flowering time in rice, a short-day plant. Plant Physiology 127, 1425-1429.


  • There are currently no refbacks.