Sugiyono Saputra, Alida Hanoum, Mulyadi Mulyadi, Achmad Dinoto
| Abstract views: 145


Xylanases are hydrolytic enzymes which randomly cleave the β-1,4 backbone of the complex plant cell wall polysaccharide xylan. In this study, we investigate the extracellular enzyme activity of three strains of Bacillus altitudinis which produced with various production times in neutral (pH 7) and alkaline (pH 9) conditions. Crude enzyme was obtained from inoculated production medium containing 2% w/v xylan after six different time of incubations, including 0, 24, 48, 72, 96 and 120 hours.  The stationary phase of those three strains was observed after 72 hours of incubation, in accordance with significant increase of xylanase activity both in neutral and alkaline assay conditions. However, the highest of xylanase activity was obtained after 92 hours of incubation, in all three bacterial strains tested. A thin layer chromatography (TLC) was performed, and it confirmed that the crudes enzymes was able to breakdown the xylan into oligosaccharides. A higher activity of xylanase was obtained in alkaline condition but not significantly different between those two conditions. B. altitudinis KBX08 has the highest xylanase activity (46.9U/ml) which produced after 96 hours of incubation, indicating their potential for further development as xylanase producer. 


Xilanase, xilan, Bacillus altitudinis, production time, pH


Alonso, JL, H Domínguez, G Garrote, JC Parajó, MJ Vázquez. 2003. Xylooligosaccharides: properties and production technologies. Electron. J. Environ. Agric. Food Chem. 230-232.

Collins, T., C. Gerday, G. Feller. 2005. Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiology Reviews (29), 3–23.

Bocchini, D.A., H.F. Alves-Prado, L.C. Baida, I.C. Roberto, E. Gomes, R. Da Silva. 2002. Optimization of xylanase production by Bacillus circulans D1 in submerged fermentation using response surface methodology. Process Biochemistry 38, 727-731.

Cossona, T, AM PeÂrez Vendrellb, B GonzaÂlez Teresac, D RenÄeÂb, P. Tailladea, J. Brufau. 1999. Enzymatic assays for xylanase and b-glucanase feed enzymes. Animal Feed Science and Technology 77,345-353.

Duarte, MCT, ACA Pellegrino, EP Portugal, Alexandre, N Ponezi; TT Franco. 2000. Characterization of Alkaline Xylanase from Bacillus altitudinis. Brazilian Journal of Microbiology 31, 90-94.

Duarte, MCT, EC da Silva, IM de Bulhoes Gomes, AN Ponezi, EP Portugal, JR Vicente, E Davanzo. 2003. Xylan-hydrolyzing enzyme system from Bacillus altitudinis CBMAI 0008 and its effects on Eucalyptus grandis kraft pulp for pulp bleaching improvement. Bioresource Technology (88),9–15.

Flores ME, R Perez, C Huitron. 1997. β-Xylosidase and xylanase characterization and production by Streptomyces sp. CH-M-1035. Lett Appl Microbiol 24:410-416.

Heck, JX, LH de Barros Soares, MAZ Ayub. 2005. Optimization of xylanase and mannanase production by Bacillus circulans strain BL53 on solid-state cultivation. Enzyme and Microbial Technology 37, 417–423.

Irfan, M., Asghar, U, Nadeem, M, Nelofer, R and Syed, Q. 2016. Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation. Journal of Radiation Research and Applied Sciences Vol 9:2 p 139-147.

Katapodis, P, V Christakopoulou, D Kekos, P Christakopoulos. 2007. Optimization of xylanase production by Chaetomium thermophilum in wheat straw using response surface methodology. Biochemical Engineering Journal 35, 136–141.

Phitsuwan, P, C Tachaapaikoon, A Kosugi, Y Mori, KL Kyu, and K Ratanakhanokchai. 2010. A Cellulolytic and Xylanolytic Enzyme Complex from an Alkalothermoanaerobacterium, Tepidimicrobium xylanilyticum BT14. J. Microbiol. Biotechnol.20(5), 893–903.

Saputra, S and Dinoto, A. 2021. Cellulolytic and xylanolytic faecal bacteria from tedong bonga [Toraja buffalo, Bubalus bubalis carabanesis]. IOP Conference Series: Earth and Environmental Science 741 (1), 012064.

Widjaja, S. T Purwadaria and PP Ketaren. 2008. Apparent Induction of Xylanase by Bacillus altitudinis PU4-2 using Pretreated Substrates. Journal Microbiol Indones. 44-48.

Yang, H, K Wang, X Song, F Xu. 2011. Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa. Bioresource Technology 102, 7171–7176.

Yoon, Young Mi, Gi Hong An , Jung Kon Kim , Seung-Hyun Ahn , Young-Lok Cha , Jungwoo Yang , Kyeong-Dan Yu , Jong-Woong Ahn , Youn-Ho Moon , Bon-Cheol Koo , and In-Hoo Choi. 2014. Xylanase Activity of Bacillus pumilus H10-1 Isolated from Ceratotherium simum Feces. Korean Society for Biotechnology and Bioengineering Journal 29(5): 316-322.


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