Shoot Tip Culture: A Potential In Vitro Culture Multiplication of Screw Pines (Pandanus tectorius Park.)
Ikhsan - Matondang, Sisunandar ., Alice Yuniaty, Triani Hardiyati, Agus Hery Susanto, Endang Srimurni Kusmintarsih
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Abstract
Pandanus tectorius Park., tree of life, which almost all of its parts are used by humans. Industrial fiber made from screw pine leaves has been successfully exported to several countries, while the fruit is consumed as an alternative staple food. Fruit is only produced by female plants, because screw pine is dioecious plant. Farmers obtain screw pine seedling using stem cuttings from female trees, but the number of seedling produced is limited and this technique can damage the plant. Alternative seedling production using seeds has constraints in the form of uncertainty over the sex of the seedling. One of the best ways to provide screw pine seedling is to use a shoot-tip culture technique. Shoot-tip culture is able to reproduce plants efficiently and quickly and does not damage the plant. Seedling will also have the same genetic traits as the selected plant. However, efforts to develop an efficient and fast screw pine shoot-tip culture protocol have not been carried out massively. The induction and shoot multiplication stages still had a low success rate. Another problem in seedling production through tissue culture is the certainty seedlings are true-to-type. This review article aims to reveal the potential of shoot-tip culture for in vitro screw pine seedling production and to test the genetic stability of the screw pine seedling.
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References
Adkar, P. P., & Bhaskar, V. H. (2014). Pandanus odoratissimus (Kewda): A review on ethnopharmacology, phytochemistry, and nutritional aspects. In Advances in Pharmacological Sciences (pp. 1–19). https://doi.org/10.1155/2014/120895
Ahmad, I., Jaskani, M. J., Nafees, M., Ashraf, I., & Qureshi, R. (2016). Control of media browning in micropropagation of guava (Psidium guajava L.). Pakistan Journal of Botany, 48(2), 713–716.
Al-Qurainy, F., Nadeem, M., Khan, S., Alansi, S., Tarroum, M., Al-Ameri, A. A., Gaafar, A. R. Z., & Alshameri, A. (2018). Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi Journal of Biological Sciences, 25, 111–116. https://doi.org/10.1016/j.sjbs.2017.07.003
Al-Taleb, M. M., Hassawi, D. S., & Abu-Romman, S. M. (2011). Production of virus free potato plants using meristem culture from cultivars grown under Jordanian environment. J. Agric. & Environ. Sci, 11(4), 467–472.
Andriani, Y., Pangestika, I., Oksal, E., Mohamad, H., Amir, H., Muhammad, T. S. T., & Wahid, M. E. A. (2019). Anti-atherosclerosis potency of Pandanus tectorius fruit rich by trangeretin and ethyl trans-caffeate, and their cytotoxicity against HepG2 cell line. IOP Conference Series: Materials Science and Engineering, 1–7. https://doi.org/10.1088/1757-899X/509/1/012155
Andriani, Y., Ramli, N. M., Syamsumir, D. F., Kassim, M. N. I., Jaafar, J., Aziz, N. A., Marlina, L., Musa, N. S., & Mohamad, H. (2019). Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits. Arabian Journal of Chemistry, 12, 3555–3564. https://doi.org/10.1016/j.arabjc.2015.11.003
Ashish, S. S., Vidyasagaran, K., Kumar, V., & Ajeesh, R. (2015). Evaluation of leaf biomass production and fibre properties of Pandanus tectorius in the coastal plains of Thrissur District, Kerala, India. Indian Journal of Tropical Biodiversity, 23(1), 69–73.
Backer, C. A., & Brink, R. C. B. van den. (1966). Flora of JavaFlora of Java (Spermatophytes Only). Taxon. https://doi.org/10.2307/1216480
Bara, D. A., Redjeki, S., & Hariadi. (2013). Studi habitat peneluran penyu hijau (Chelonia mydas) di pantai pangumbahan Sukabumi Jawa Barat. Journal Of Marine Research., 2(3), 147–155.
Bhatia, S., & Bera, T. (2015). Somatic embryogenesis and organogenesis. In K. Bhatia, Saurabh; Bera, Tanmoy;Dahiya, Randhir; Sarma (Ed.), Modern Applications of Plant Biotechnology in Pharmaceutical Sciences (pp. 209–230). https://doi.org/10.1016/B978-0-12-802221-4.00006-6
Bhuiyan, F. R. (2013). In vitro meristem culture and regeneration of three potato varieties of Bangladesh. Research in Biotechnology, 4(3), 29–37.
Biradar, S., Biradar, D. P., Patil, V. C., Patil, S. S., & Kambar, N. S. (2009). In vitro plant regeneration using shoot tip culture in commercial cultivar of sugarcane. Karnataka J. Agric. Sci., 22(1), 21–24.
Burri, J., Graf, M., Lambelet, P., & Löliger, J. (1989). Vanillin: more than a flavouring agent—a potent antioxidant. Journal of the Science of Food and Agriculture. https://doi.org/10.1002/jsfa.2740480107
Cahyaningsih, Y. F., Wiendi, N. M. A., & Toruan-Mathius, D. N. (2016). Deteksi kestabilan genetik ramet kelapa sawit hasil kultur in vitro menggunakan SSR. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 44(1), 83–90. https://doi.org/10.24831/jai.v44i1.12507
Çetin, B. (2018). Evaluation of the genetic fidelity of in vitro raised plants of Origanum majorana L. using random amplified polymorphic DNA. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14(2), 237–239. https://doi.org/10.18466/cbayarfbe.406873
Cruz-Martínez, V., Castellanos-Hernández, O. A., Acevedo-Hernández, G. J., Torres-Morán, M. I., Gutiérrez-Lomelí, M., Ruvalcaba-Ruiz, D., Zurita, F., & Rodríguez-Sahagún, A. (2017). Genetic fidelity assessment in plants of Sechium edule regenerated via organogenesis. South African Journal of Botany, 112(October), 118–122. https://doi.org/10.1016/j.sajb.2017.05.020
Dellaporta, S. L., & Calderon-Urrea, A. (1993). Sex determination in flowering plants. In Plant Cell. https://doi.org/10.1105/tpc.5.10.1241
Devy, N. F., Yulianti, F., & Hardiyanto, H. (2012a). Pengaruh densitas awal kalus dalam perbanyakan melalui embriogenesis somatik terhadap daya multiplikasi dan stabilitas genetik planlet siam kintamani. Jurnal Hortikultura, 22(4), 309–315. https://doi.org/10.21082/jhort.v22n4.2012.p309-315
Devy, N. F., Yulianti, F., & Hardiyanto, H. (2012b). Perbanyakan massal embrio kalamondin melalui teknologi somatik embriogenesis menggunakan bioreaktor. Jurnal Hortikultura, 22(1), 1–7. https://doi.org/10.21082/jhort.v22n1.2012.p1-7
Doule, R. B., Kawar, P. G., Devarumath, R. M., & Nerkar, Y. S. (2008). Field performance and RAPD analysis for assessment of genetic variation in sugarcane somaclones. Indian Journal of Genetics and Plant Breeding, 68(3), 301–306.
Englberger, L., Schierle, J., Hofmann, P., Lorens, A., Albert, K., Levendusky, A., Paul, Y., Lickaneth, E., Elymore, A., Maddison, M., DeBrum, I., Nemra, J., Alfred, J., Velde, N. Vander, & Kraemer, K. (2009). Carotenoid and vitamin content of Micronesian atoll foods: Pandanus (Pandanus tectorius) and garlic pear (Crataeva speciosa) fruit. Journal of Food Composition and Analysis, 22, 1–8. https://doi.org/10.1016/j.jfca.2008.12.001
Febrina, E., Dana, N., Anas, S., & Destiani, D. P. (2016). Aktivitas analgesik ekstrak fraksi N-heksana, etil asetat, dan air buah pandan laut (Pandanus tectorius) pada mencit metode geliat. Farmaka, 14(2), 1–10.
Ferdous, M. H., Masum Billah, A. A., Mehraj, H., Taufique, T., & Jamal Uddin, A. F. M. (2015). BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. Journal of Bioscience and Agriculture Research, 03(02), 87–95. https://doi.org/10.18801/jbar.030215.35
Gallaher, T. (2014). The past and future of hala (Pandanus tectorius) in Hawai‘i. In ’Ike Ulana Lau Hala: The Vitality and Vibrancy of Lau Hala Weaving Traditions in Hawai’i (pp. 1–18). https://doi.org/10.21313/hawaii/9780824840938.003.0008
Gangopadhyay, G., Bandyopadhyay, T., Modak, B. K., Wongpornchai, S., & Mukherjee, K. K. (2004). Micropropagation of Indian pandan (Pandanus amaryllifolius Roxb.), a rich source of 2-acetyl-1-pyrroline. Current Science, 87(11), 1589–1592.
George, E. F. (2008). Plant tissue culture procedure-background. In G.-J. George, E F.; Hall, Ml A.;De Klerk (Ed.), Plant Propagation by Tissue Culture 3rd Edition. Springer. https://doi.org/10.1007/978-1-4020-5005-3
Gong, S., Yang, Z., & J, C. (2006). Inhibition of tyrosinase activity by vaniliin. Journal of South China University of Technology.
Goyal, A. K., Pradhan, S., Basistha, B. C., & Sen, A. (2015). Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers. 3 Biotech, 5, 473–482. https://doi.org/10.1007/s13205-014-0244-7
Gurmeet, S., & Amrita, P. (2015). Unique pandanus -flavour, food and medicine. Journal of Pharmacognosy and Phytochemistry JPP, 5(3), 08–14.
Halušková, J., & Košuth, J. (2003). RAPD analysis of somaclonal and natural DNA variation in Hypericum perforatum L. Acta Biologica Cracoviensia Series Botanica, 45(2), 101–104.
Hani, A., & Dendang, B. (2008). Teknik pembibitan pandan Pandanus tectorius Parkinson ex Z. Balai, V(3), 255–260.
Heyne, K. (1987). Tumbuhan berguna Indonesia, jil. 3, terjemahan Badan Litbang Kehutanan Jakarta. In Yayasan Sarana Warna Jaya, Jakarta.
Hoa, N. T., Dien, P. H., & Quang, D. N. (2014). Cytotoxic steroids from the stem barks of Pandanus tectorius. Research Journal of Phytochemistry, 8(2), 52–56. https://doi.org/10.3923/rjphyto.2014.52.56
Holle, M. J. ., Farkhah, Y., Arfriana, F. R., & Nuriliani, A. (2013). Cytotoxic activity and apoptotic induction of leaves and fruit extract of screw pine (Pandanus tectorius) to T47D cell line. The 1st Annual International Scholars Conference in Taiwan., April, 580–586.
Indaryani, P. M. K. ; Ashari, S., & dan Mariana, B. D. (2019). Analysis genetic diversity of grape (Vitis vinivera L.) varieties BS 60 from tissue culture propagation with ISSR markers. Jurnal Produksi Tanaman, 7(11), 2100–2106.
Isda, M. N., Fatonah, S., & Sari, L. N. (2016). Pembentukan tunas dari biji manggis (Garcinia mangostana L.) asal Bengkalis dengan penambahan BAP dan madu secara in vitro. Al-Kauniyah: Jurnal Biologi, 9(2), 119–124. https://doi.org/10.15408/kauniyah.v9i2.3376
Jafari, N., Othman, R. Y., & Khalid, N. (2011). Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan. African Journal of Biotechnology, 10(13), 2446–2450. https://doi.org/10.5897/AJB10.1149
Jaisankar, I., Jerard, B. ., Velmurugan, A., & Swarnam, T. . (2020). Analysis of genetic relationship of Pandanus tectorius accessions of Andaman and Nicobar Islands using RAPD and ISSR markers. Indian J. of Agroforestry, 22(1), 24–30.
Jose, B., Harikumar, K., Krishnan, P. N., & Satheeshkumar, K. (2016). In vitro mass multiplication of screw pines (Pandanus spp.) - an important costal bio- resource. Journal of Coastal Conservation, 20, 443–453. https://doi.org/10.1007/s11852-016-0458-4
Kaushal, S., Sidana, A., & Dev, K. (2015). In vitro plant production through apical meristem culture of Gentiana kurroo Royle. Journal of Medicinal Plants Studies, 3(1), 04–09.
Khan, S. A., Rashid, H., Chaudhary, M. F., Chaudhry, Z., & Afroz, A. (2008). Rapid micropropagation of three elite Sugarcane (Saccharum officinarum L.) varieties by shoot tip culture. African Journal of Biotechnology, 7(13), 2174–2180. https://doi.org/10.5897/AJB08.869
Khoo, B. Y., Chua, S. L., & Balaram, P. (2010). Apoptotic effects of chrysin in human cancer cell lines. International Journal of Molecular Sciences, 11, 2188–2199. https://doi.org/10.3390/ijms11052188
Ko, C. Y., Al-Abdulkarim, A. M., Al-Jowid, S. M., & Al-Baiz, A. (2009). An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. African Journal of Biotechnology, 8(11), 2611–2615. https://doi.org/10.4314/ajb.v8i11.60783
Kumar, N., Modi, A. R., Singh, A. S., Gajera, B. B., Patel, A. R., Patel, M. P., & Subhash, N. (2010). Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay. Physiology and Molecular Biology of Plants, 16(2), 207–212. https://doi.org/10.1007/s12298-010-0023-9
Lee, S. H., Park, Y. B., Bae, K. H., Bok, S. H., Kwon, Y. K., Lee, E. S., & Choi, M. S. (1999). Cholesterol-lowering activity of naringenin via inhibition of 8-hydroxy-3-methylglutaryl coenzyme a reductase and acyl coenzyme A:cholesterol acyltransferase in rats. Annals of Nutrition and Metabolism, 43(3), 173–180. https://doi.org/10.1159/000012783
Lengkong, C. L. W., Polii-Mandang, J., & Lengkong, E. F. (2012). Identifikasi keragaman genetik kentang supejohn transgenik (Solanum tuberosum L. var supejohn). EUGENIA, 18(3), 197–204. https://doi.org/10.35791/eug.18.3.2012.4095
Lestari, E. G. (2011). Peranan zat pengatur tumbuh dalam perbanyakan tanaman melalui kultur jaringan. Jurnal AgroBiogen, 7(1), 63–68. https://doi.org/10.21082/jbio.v7n1.2011.p63-68
Liu, H., Zhang, X., Wu, C., Wu, H., Guo, P., & Xu, X. (2013). Anti-hyperlipidemic caffeoylquinic acids from the fruits of Pandanus tectorius soland. Journal of Applied Pharmaceutical Science, 3(08), 016–019. https://doi.org/10.7324/JAPS.2013.3803
Mahlinda, Supardan, M. D., Husin, H., & Riza, M. (2016). In situ transesterication of screw pine seed (Pandanus tectorius) to biodiesel using ultrasound. Jurnal Teknologi Industri Pertanian, 26(3), 294–300.
Majumdar, K., Saha, R., Datta, B., & Bhakta, T. (2006). Medicinal plants prescribed by different tribal and non-tribal medicine men of Tripura state. Indian Journal of Traditional Knowledge (IJTK), 5(4), 28.
Manuel, J., & George, P. (2013). Influence of apical meristem and chemotherapy on production of virus free sugarcane plants. Research Journal of Recent Sciences Res. J. Recent. Sci, 3, 305–309.
Martinalova, D. (2004). Pemanfaatan kulit buah Pandanus tectorius sebagai pewarna pada pembuatan lipstik. In Skripsi Institut Pertanian Bogor.
Mateu-Andrés, I., & De Paco, L. (2004). Allozymic differentiation of the Antirrhinum majus and A. siculum species groups. Annals of Botany, 465–473. https://doi.org/10.1093/aob/mci055
Mondini, L., Noorani, A., & Pagnotta, M. A. (2009). Assessing plant genetic diversity by molecular tools. Diversity, 1(1), 19–35. https://doi.org/10.3390/d1010019
Nadeem, M. A., Nawaz, M. A., Shahid, M. Q., Doğan, Y., Comertpay, G., Yıldız, M., Hatipoğlu, R., Ahmad, F., Alsaleh, A., Labhane, N., Özkan, H., Chung, G., & Baloch, F. S. (2018). DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology and Biotechnological Equipment, 32(2), 261–285. https://doi.org/10.1080/13102818.2017.1400401
Nahmias, Y., Goldwasser, J., Casali, M., Van Poll, D., Wakita, T., Chung, R. T., & Yarmush, M. L. (2008). Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin. Hepatology, 47(5), 1437–1445. https://doi.org/10.1002/hep.22197
Nayanakantha, N., Singh, B., & Kumar, A. (2010). Improved culture medium for micropropagation of Aloe vera L. Tropical Agricultural Research and Extension, 13(4), 87–93. https://doi.org/10.4038/tare.v13i4.3291
Nguyen, V. H., Yen, C. R., & Hsieh, C. H. (2018). Effect of nutritional and growth hormonal factors on in vitro regeneration of papaya (Carica papaya L. cv. Red Lady). Journal of the National Science Foundation of Sri Lanka, 46(4), 559–568. https://doi.org/10.4038/JNSFSR.V46I4.8631
Nurtjahjaningsih, I., Herawan, T., Rachman, R. P., & Rimbawanto, A. (2018). Pengujian penanda random amplified polymorphism DNA untuk mengetahui kestabilan genetik klon jati (Tectona grandis). Jurnal Pemuliaan Tanaman Hutan, 12(2), 127–134. https://doi.org/10.20886/jpth.2018.12.2.127-134
Panda, K. K., Das, A. B., & Panda, B. B. (2009). Use and variation of Pandanus tectorius Parkinson (P. fascicularis Lam.) along the coastline of Orissa, India. Genetic Resources and Crop Evolution. https://doi.org/10.1007/s10722-008-9390-2
Panda, K. K., Sahoo, B., Das, A. B., & Panda, B. B. (2010). Use of RAPD markers to detect sex differences in Pandanus tectorius Parkinson, an important bioresource plant in Orissa, India. International Journal of Biodiversity Science, Ecosystem Services and Management, 1–7. https://doi.org/10.1080/17451590.2010.487472
Pant, B., & Thapa, D. (2012). In vitro mass propagation of an epiphytic orchid,Dendrobium primulinum Lindl. through shoot tip culture. African Journal of Biotechnology, 11(42), 9970–9974. https://doi.org/10.5897/ajb11.3106
Park, Y. S., Barrett, J. D., & Bonga, J. M. (1998). Application of somatic embryogenesis in high-value clonal forestry: development, genetic control, and stability of cryopreserved clones. In In Vitro Cellular and Developmental Biology - Plant (pp. 231–239). https://doi.org/10.1007/BF02822713
Phillips, R. L., Kaeppler, S. M., & Olhoft, P. (1994). Genetic instability of plant tissue cultures: breakdown of normal controls. In Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.91.12.5222
Poerba, Y. S., Imelda, M., & Martanti, D. (2012). Analisa kestabilan genetik pisang kepok ‘unti sayang’ hasil mikroprogasi dengan marka RAPD dan ISSR. Berita Biologi, 11(2), 275–282. https://www.neliti.com/publications/69068/analisa-kestabilan-genetik-pisang-kepok-unti-sayang-hasil-mikroprogasi-dengan-ma
Pranata, I. T., & Herawati, M. M. (2019). Efektivitas sterilisasi kimiawi eksplan pucuk Artemisia annua LINN. dengan berbagai prosedur sterilisasi pada tahap inisiasi in vitro. Agrium, 22(2), 94–101.
Qian, W., Ge, S., & Hong, D. Y. (2001). Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theoretical and Applied Genetics, 102, 440–449. https://doi.org/10.1007/s001220051665
Rahayu, M., Sunarti, S., & Keim, A. R. Y. P. (2008). Kajian etnobotani pandan samak (Pandanus odoratissimus L . f .): pemanfaatan dan peranannya dalam usaha menunjang penghasilan keluarga di Ujung Kulon , Banten. Biodiversitas, 9(4), 310–314.
Rahayu, S. ., Sinaga, E., & Noverita. (2015). Kajian Pandanus tectorius dalam upaya mitigasi tsunami dan pemanfaatannya sebagai tumbuhan obat dan bahan kerajinan anyama. Laporan Akhir Penelitian Strategis Nasional, 114.
Rahayu, S. E., & Handayani, S. (2008). Keanekaragaman morfologi dan anatomi Pandanus (Pandanaceae) di Jawa Barat. VIS VITALIS, 01(2), 29–44.
Rahayu, S. E., Sinaga, E., Herzagovina, S., Noverita, & Jaelani, M. R. (2019). Phytochemical constituents, antioxidant, antibacterial and cytotoxicity properties of Pandanus tectorius. Systematic Reviews in Pharmacy, 10(1), 289–295. https://doi.org/10.5530/srp.2019.1.45
Rajeswari, J., Kesavan, K., & Jayakar, B. (2012). Antidiabetic activity and chemical characterization of aqueous/ethanol prop roots extracts of Pandanus fascicularis Lam in streptozotocin-induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine, 2(1), S170–S174. https://doi.org/10.1016/S2221-1691(12)60152-X
Rani, V., & Raina, S. . (2000). Genetic fidelity of organized meristem-derived micropropagation plants: A criticalreappraisail. In Vitro Cellular & Developmental Biology - Plant, 36(5), 319–330.
Roemantyo, Nastiti, A. S., & Wiadnyana, N. N. (2012). Struktur dan komposisi vegetasi sekitar sarang penyu hijau (Chelonia mydas Linnaeus) pantai Pangumbahan, Sukabumi Selatan, Jawa Barat. Berita Biologi, 11(3), 373–387.
Roostika, I., Mariska, I., & Purnamaningsih, R. (2005). Regenerasi tanaman sedap malam melalui organogenesis dan embriogenesis somatik. Jurnal Hortikultura, 15(4), 233–241. https://doi.org/10.21082/jhort.v15n4.2005.p%p
Sarungallo, Z. L., Susanti, C. M. E., Sinaga, N. I., Irbayanti, D. N., & Latumahina, R. M. M. (2018). Kandungan gizi buah pandan laut (Pandanus tectorius Park.) pada tiga tingkat kematangan. Jurnal Aplikasi Teknologi Pangan, 7(1), 21–26. https://doi.org/10.17728/jatp.2577
Sarwat, M., Nabi, G., Das, S., & Srivastava, P. S. (2012). Molecular markers in medicinal plant biotechnology: past and present. In Critical Reviews in Biotechnology (pp. 1–19). https://doi.org/10.3109/07388551.2011.551872
Semagn, K., Bjørnstad, Å., & Ndjiondjop, M. N. (2006). An overview of molecular marker methods for plants. African Journal of Biotechnology, 5(25), 2540–2568. https://doi.org/10.5897/AJB2006.000-5110
Shatnawi, M., Anfoka, G., Shibli, R., Al-Mazra’Awi, M., Shahrour, W., & Arebiat, A. (2011). Clonal propagation and cryogenic storage of virus-free grapevine (Vitis vinifera L.) via meristem culture. Turkish Journal of Agriculture and Forestry, 35, 173–184. https://doi.org/10.3906/tar-0912-519
Singh, A., Negi, M. S., Moses, V. K., Venkateswarlu, B., Srivastava, P. S., & Lakshmikumaran, M. (2002). Molecular analysis of micropropagated neem plants using AFLP markers for ascertaining clonal fidelity. In Vitro Cellular and Developmental Biology - Plant, 38(5), 519–524. https://doi.org/10.1079/IVP2002341
Singh, P., & Patel, R. M. (2014). Factors influencing in vitro growth and shoot multiplication of pomegranate. The Bioscan, 9(3), 1031–1035. http://www.thebioscan.in/Journals_PDF/9323 2772_PUSHPRAJ SINGH.pdf
Sirisom, Y., & Te-Chato, S. (2012). The effect of peptone and silver nitrate on In vitro shoot formation in Hevea brasiliensis Muell Arg. Journal of Agricultural Technology, 8(4), 1509–1516.
Sisunandar, Rival, A., Turquay, P., Samosir, Y., & Adkins, S. W. (2010). Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos does not induce morphological, cytological or molecular changes in recovered seedlings. Planta, 232, 435–447. https://doi.org/10.1007/s00425-010-1186-x
Skoog, F., & Miller, C. O. (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology, v(11), 118–131.
Sughra, M. G., Altaf, S. A., Rafique, R. M., Muhammad, M. S., Rind Balouch, S. N., & Umar, D. M. (2014). In vitro regenerability of different sugarcane (Saccharum officinarum L.) varieties through shoot tip culture. Pakistan Journal of Biotechnology, 11(1), 13–23.
Sukarno, N. S. (2016). Sintesis membran elektrolit selulosa asetat dari daun pandan laut (Pandanus tectorius) dengan pemlastis DBP (Dibuthylphthalate) untuk aplikasi baterai ion lithium. In Skripsi Jurusan Kimia Universitas Negeri Yogyakarta.
Suman, S. (2017). Plant tissue culture: A promising tool of quality material production with special reference to micropropagation of banana. In Biochemical and Cellular Archives (pp. 1–26).
Susiarti, S., & Rahayu, M. (2010). Kajian etnobotani pandan samak (Pandanus tectorius Sol.) di kabupaten Tasikmalaya, Jawa Barat. Berita Biologi, 10(1), 113–121.
Tarampak, T. C., Sulistiawati, S., & Nirmala, R. (2019). Metode mengatasi browning pada eksplan ulin (Eusideroxylon zwageri) untuk inisiasi regenerasi secara in vitro. Jurnal Agroekoteknologi Tropika Lembab, 1(2), 106–117. https://doi.org/10.35941/jatl.1.2.2019.1972.106-113
Thomson, L. A. J., Englberger, L., Guarino, L., Thaman, R. R., & Elevitch, C. R. (2006). Pandanus tectorius (Pandanus) Pandanaceae (screwpine family). In Species Profiles for Pacific Island Agroforestry www.traditionaltree.org (Issue April ver 1.1, pp. 1–29).
Thorpe, T. A. (2007). History of plant tissue culture. Molecular Biotechnology, 37(2), 169–180. https://doi.org/10.1007/s12033-007-0031-3
Tilkat, E., Işikalan, Ç., & Onay, A. (2005). In vitro propagation of khinjuk pistachio (Pistacia khinjuk Stocks) through seedling apical shoot tip culture. Propagation of Ornamental Plants, 5(3), 1–5.
Tyagi, H., & Tomar, U. K. (2013). Factors affecting in vitro shoot proliferation and rooting of mature Tecomella undulata ( Sm .) seem tree. Research in Plant Sciences, 1(2), 38–44.
Venkatesh, S., Kusuma, R., Sateesh, V., Madhava, R. B., & Mullangr, R. (2012). Antidiabetic activity of Pandanus odoratissimus root extract. Indian Journal of Pharmaceutical Education and Research, 46(4), 340–345. https://doi.org/10.5530/ijper.47.3.4
Vinod, M. S., Raghavan, P. S., George, S., & Parida, A. (2007). Identification of a sex-specific SCAR marker in dioecious Pandanus fascicularis L. (Pandanaceae). Genome, 50, 834–839. https://doi.org/10.1139/G07-066
Vuylsteke, D. R. (1989). Shoot-tip culture for the propagation, conservation and exchange of Musa germplasm. Practical Manuals for Handling Crop Germplasm In Vitro, 2, 1–56.
Widiastuti, A., Sobir, & Suhartanto, M. (2013). Analisis keragaman manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan penanda ISSR. Bioteknologi, 10(1), 15–22.
Xiaopo Zhang; Peng Guo; Guangli Sun; Shilin Chen; Meihua Yang; Naiguang Fu; Haifeng Wu and Xudong Xu. (2012). Phenolic compounds and flavonoids from the fruits of Pandanus tectorius Soland. Journal of Medicinal Plants Research, 6(13), 2622–2626. https://doi.org/10.5897/jmpr11.1424
Yadav, A., Kothari, S. L., Kachhwaha, S., & Joshi, A. (2019). In vitro propagation of chia (Salvia hispanica L.) and assessment of genetic fidelity using random amplified polymorphic DNA and intersimple sequence repeat molecular markers. Journal of Applied Biology and Biotechnology, 7(1), 42–47. https://doi.org/10.7324/JABB.2019.70108
Yulianti, F., Arisah, H., & Agisimanto, D. (2018). Pengujian stabilitas genetik plantlet citrumelo hasil tTCL dari kultur in vitro dengan menggunakan teknik sekuen berulang. Jurnal Hortikultura, 27(2), 165–172. https://doi.org/10.21082/jhort.v27n2.2017.p165-172
Zainal, A., Anwar, A., & Kasim, M. (1999). Pengaruh NAA dan kinetin terhadap pertumbuhan pandan (Pandanus tectorius) secara in vitro. Stigma Volume VII No.1: 90-94., VII(1), 90–94.
Zietkiewicz, E., Rafalski, A., & Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20, 176–183. https://doi.org/10.1006/geno.1994.1151
Zucchi, M. I., Arizono, H., Morais, V. A., Fungaro, M. H. P., & Vieira, M. L. C. (2002). Genetic instability of sugarcane plants derived from meristem cultures. Genetics and Molecular Biology, 25(1), 91–96. https://doi.org/10.1590/S1415-47572002000100017
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