Aktivitas Anti Inflamasi Ekstrak Biji Litsea Garciae Vidal Pada Odema Telapak Kaki Dan Gambaran Histologis Kaki Mencit

Mustika Sari, Henny Sulistiany
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Abstract

Litsea garciae or malek is a native species from Borneo and belongs to the Lauraceae family. Scientifically, the use of these plants is not widely known. The purpose of this study was to determine the anti-inflammatory activity of Litsea garciae seeds against edema in mice feet and to determine the histology of the carrageenan-induced integument thickness of mice. The study used a completely randomized design with 5 treatment groups. The treatment group consisted of a negative treatment group (K1), a positive treatment group (K2) with 3 different dosages of Malek seed extract groups. The treatment doses used were Malek seed extract at a dose of 0.625 mg/g BW (P1), 1.25 mg/g BW (2), and 2.50 mg/g BW (P3). Edema percentage and integument thickness were analyzed by the One-Way ANOVA test with (α = 0.05). The results showed that the Malek seed extract dose of 0.625 mg/g BW had anti-inflammatory activity against edema of the mice's feet. The percentage of inflammation with doses of 0.625 mg/g BW, 1.25 mg/g BW and 2.5 mg/g BW was 31.10%, 22.58%, and 25.83%. The percentage of reduction in inflammation in the positive control treatment of Na-diclofenac (42.70%) was significantly differ-ent from the treatment of Malek seed extract at a dose of 0.0625–2.5 mg/g BW. The percentage of reduction in inflammation in the group treated with 1.25 mg/g BW (P2) Malek seed extract was not significantly different from the 2.5 mg/g BW (P3) treatment of Malek seed extract. The results of histological observations of mice's feet showed that the extract of Malek seeds did not have a significant effect on the thickness of the carrageenan-induced mice leg integument.

Keywords

Inflamation; Litseagarciae; Odema; Histological Structure

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References

Agrawal, N., Choudhary, AS., Sharma, MC., Dobhal. 2011. Chemical Constituents Of Plants From The Genus Litsea. Chemistry & Biodiversity.8

Andrie & Idiawati (2014). Aktivitas Antioksidan Dan Sitotoksisitas Ekstrak Daun Malek (Litsea Garciae Vidal). Jurnal Khatulistiwa. 3(4):21-25

Akmal, A., Kuswanto., Fahrunnisa, S., Rahmi., Bayanil., Anni Nurliani. 2016. Efek Spermisida Ekstrak Metanol Biji Buah Kalangkala (Litsea Angulata) Terhadap Spermatozoa Mencit (Mus Musculus). Journal Of Agromedicine And Medical Sciences. 2 (2).

Artis, D., Spits, H. 2015. The Biology Of Innate Lymphoid Cells. Nature. 517: 293–301

Azhar, M, Salleh., W. 2020 Chemical Composition And Biological Activities Of Essential Oils Of The Genus Litsea (Lauraceae) – A Review. Agric Conspec. 85: 97-103

Baratawidjaja, K.G. 2002. Imunologi Dasar. Edisi Kelima. Balai Penerbit Fakultas Kedokteran Universitas Indonesia. Jakarta.

Corwin, E. J., 2008, Handbook Of Pathophysiology, Wolters Kluwer Health/Lippincott Williams Dan Wilkins, Philadelphia.

Devib, P.,Meera,R.,2010.Study of antioxdant, Antiinflammatory And woundhealing Activity Ofextractsof Litsea Glutinosa. Journal of pharmaceutical sciences and Research2,155–163.

Dorland, W. A. N., 2002,Kamus Kedokteran Dorland. (Penerjemah: Setiawan, A., Banni, A. P., Widjaja, A. C., Adji, A. S., Soegiarto, B., Kurniawan, D., Dkk.), Egc, Jakarta.

Elmitra, E., Apriyanti, O., & Sepriani, T. L. (2019). Uji Efektivitas Antiinflamasi Ekstrak Etanol Daun Cabe Rawit (Solanum Frutescens. L) Pada Mencit Jantan (Mus Muscullus) Dengan Metode Induksi Caraagenan. Journal Academi Pharmacy Prayoga, 4(2), 1-13.

Hassan, S.H., Jeffrey,Fry., Abu, M.F. 2013. Antioxidant And Phytochemical Study On Pengolaban (Litsea garciae) An Edible Underutilized Fruit Endemic To Borneo. Food Sci. Biotechnol. 22(5): 1197-1203.

Indriyani, 2015. Buah Litses garciae, buah eksotik asal Kalimantan. Balai Penelitian Tanaman Buah Tropika. Hortikulturano.

Kong, A., et al. 2015. The Genus Litsea In Traditional chinese medicine:Anethnomedical, Phytochemicalandpharmacologicalreview. Journal Of ethnopharmacology. 113-120

Kuspradini., Wulandari., Putri, AS., Tiya, SY. 2019. Phytochemical, Antioxidant And Antimicrobial Properties Of Litsea Angulata Extracts. F1000research. 7:1839-1849

Katzung B. G. (2002). Farmakologi Dasar Dan Klinik Edisi 8. Salemba Medika. Jakarta.

Lim, Tk 2012, Litsea Garciae, In Fruits, Edible Medicinal And Non Medicinal Plants, Volume 3, Springer Science + Business Media B.V, Pp. 898

Morris, Crhristoper J. (2003). CarrageenanInduced Paw Edema in the Rat and Mouse, In P.G. Winyard and D.A. Willoughby (Ed). Method in Molecular Biology. Vol. 225 : Inflammation Protocols. Totowa. NJ :Humana Press Inc.

Necas, J., Bartosikova, L. 2013. Carrageenan: A Review, Faculty Of Medicine And Dentistry. Palacky University. Olomouc. Czech Republic : Veterinarni Medicina. 58 (4): 187–205.

Nijveldt, R.J.E, Van, N.D, Hoorn, P,G, Boelens, K, Van, P.A, Dan Van, L. (2001). Flavonoid: A Review Of Probable Mechanisms Of Action And PotentialApplications. American Journal Of Clinical And Nutrition. 74

Nuroini, F & Nastiti (2017). Uji Efek Antiinflamasi Sarang Burung Walet (Collocalia Fuciphaga Thunberg) Terhadap Gambaran Histologis Telapak Kaki Mencit (Mus Musculus Linneaus). Jurnal Labora Medika.1 (1) 21-26

Octasari, P. M., & Djunarko, I. (2012). Efek Anti-Inflamasi Benzoil Eugenol secara Topikal terhadap Edema Kaki yang Diinduksi Formalin 0, 5% pada Mencit Jantan Galur Swiss. Jurnal Farmasi Sains dan Komunitas, 9(1), 26-35.

Ravi, V., Saleem, T.S.M., Patel, S.S., Raamamurthy, J. Dan Gauthaman, K. 2009. Anti-Inflammatory Effect Of Methanolic Extract Of Solanum Nigrum Linn. Berries. Inter. J. App. Res. Nat. Prod. 2 (2): 33-36.

Santoso, P., Sari., WB., Kusuma, Y. 2018. Skrining Fitokimia Dan Uji Aktivitas Antiinflamasi Ekstrak N-Butanol Buah Dewandaru (Eugenia Uniflora L.) Pada Tikus Putih Jantan Galur Wistar (Rattus Norvegicus) Dengan Metode Paw Edema Yang Diinduksi Karagenan. Medicamento. 4(2): 2356-4818

Saputri, R & Susiani, EF. 2018. Uji Aktivitas Antioksidan dan Ekstrak Etanol Buah dan Biji Buah Kalangkala (Listea angulata) Asal Kalimantan Selatan. Borneo Journal of Pharmacy: I(2): 81 – 84

Sari, M & Ariyani., D. 2016. Dahlena Skrining Fitokimia Ekstrak Metanol Biji Kalangkala (Litsea Angulata). Sains Dan Terapan Kimia. 4(2):131-136

Sentat Triswanto dan Handayani Fitri.2018.Uji Efek Antinflamasi Ekstrak Etanol Biji Lamtoro (Leucaena leucocephala L) Terhadap Udem Telapak Kaki Mencit yang Diinduksi Karagenan.Akademi Farmasi Samarinda:Kalimanta Timur

Singh, Amritpal, Maholtra, S., & Subban, R. (2008). Antiinflamatory And Analgesic Agents From Indian Medicinal Plants. International Journal Of Integrative Biology.

Susi, 2018. Identifikasi Komponen Kimia Buah Kalangkala dan Binjai Sebagai Bahan Pangan. http://eprints.ulm.ac.id/id/eprint/3563. Diakses 15 Agustus 2019

Sukmawati, Yuliet, Ririen. 2015. Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Pisang Ambon (Musa Paradisiaca L.) Terhadap Tikus Putih (Rattus Norvegicus L.) Yang Diinduksi Karagenan. Galenika Journal Of Pharmacy Vol. 1 (2) : 126 – 132

Slik J.W.F. 2006. Trees Of Sungai Wain. Http://Www.Nationaalherbarium.Nl /Sungaiwain/Main.Htm. Diakses Tanggal 10 Agustus 2019.

Suwiti, N. 2010. Deteksi Histologik Kesembuhan Luka Pada Kulit Pasca Pemberian Daun Mengkudu (Morinda Citrofilia Linn). Buletin Veteriner Udayana. Vol. 2 No.1. :1-9

Tanu, I., Syarif, A., Estuningtyas, A., Setiawati, A., Muchtar, H.A. Dan Arif, A. 2002. Farmakologi Dan Terapi. Fakultas Kedokteran Universitas Indonesia. Jakarta.

Trade Winds Fruit. Plant Informational Database, Diunduh 10 Agustus 2019, .

Umboh, R. & Rompas. Potensi Anti-Inflamasi Ekstrak Etanol Kulit BuahManggis (Garcinia Mangostana L.) Terhadap Edema Telapak Kaki Tikus Putih (Rattus Norvegicus) Yang Diinjeksi Formalin. Jurnal Sains, Matematika, & Edukasi (Jsme). (2): 211-217.

Voon B.H And Kueh H.S., 1999, Nutrional Value Of Indigenous Fruits And Vegetablesin Sarawak, Asia Pacific J C Lin Nutr,8(1):24-31.

Wilmana, F. P., 2007,Farmakologi Dan Terapi Edisi Ke-5. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia.

Yan,X.H.,Zhang,F.X.,Wei,X.Y.,Xie,H.H.,Liu,M.F.,2001.Chemical constituents in Essential Oil from the leave sof litsea rotundifoliavar Oblongifolia.J.Trop. Subtropical Bot.9,81–82.

Xu, Z., Zhou, J., Cai, J., Zhu, Z., Sun, X., Dan Jiang, C. 2012. Anti-Inflammatory Effects Of Hydrogen Saline In Lps Activated Macrophages And Carrageenan Anduced Paw Oedema. Journal Of Inflammation. 9 (2): 1-8.

Yan,X.H.,Wei,X.Y.,Xie,H.H.,Liu,M.F.,Zhang,F.X.,2000.A review of the studies on Chemical Constituents from Litsea Lam. J.Trop.Subtropicalbot.8,171–176.

Yun-Songwang A., Zheng-Qiwen B,N.,Bi-Taoli B., Hong-Binzhang A., Jing-Huayang. 2016. Ethnobotany,Phytochemistry,Andpharmacology of thegenus Litsea. Journal Of ethnopharmacology. 66–107.


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