SELECTIVE ISOLATION OF Dactylosporangium AND Micromonospora FROM THE SOIL OF KARST CAVE OF SIMEULUE ISLAND AND THEIR ANTIBACTERIAL POTENCY

Ade Lia Putri, I Nyoman Sumerta
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Abstract

Karst is a unique ecosystem that consists of a thin soil layer on the carbonate rocks. It has a diverse microorganism, especially actinomycetes group, which might potentially produce beneficial secondary metabolites that remain unknown. In this study, we were interested in isolating Dactylosporangium and Micromonospora that have been potentially reported as antibiotic sources. We used two methods using the chemical germicide (pretreatment 1.5% phenol) compared with the SDS-YE (0.05% SDS pretreatment). Thirty-nine isolates actinomycetes were successfully isolated from two samples of the karst cave soils. Thirteen isolates were obtained by using the germicide chemical method and closely related to the genera of Dactylosporangium and Micromonospora. On the other hand, the SDS-YE grew twenty-six isolates which closely related to 11 genera of actinomycetes (Catenulispora, Nocardia, Rhodococcus, Ornithinimicrobium, Catellatospora, Dactylosporangium, Micromonospora, Streptacidiphilus, Streptomyces, Nonomuraea, and Streptosporangium). These results suggest that 1.5% of phenol pretreatment could preserve the genera of Dactylosporangium and Micromonospora, while the Streptomyces and other rare actinomycetes were killed. Among all isolates, only 7 showed the antibacterial activity on tested bacteria. Even though the antibacterial activity of those isolates was not high, the isolation of actinomycetes from a specific substrate is necessary to be conducted on exploring the richness of our natural resources.

 

 

Keywords

Actinomycetes, Dactylosporangium, Micromponaspora, phenol, karst

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References

Baskaran, R., Vijayakumar, R. and Mohan, P.M., 2011. Enrichment method for the isolation of bioactive actinomycetes from mangrove sediments of Andaman Islands, Indian Journal of Microbiology, 7(1), pp. 26–32.

Borsodi, A. K., Knáb, M., Krett, G., Makk, J., Márialigeti, K., Eross, A. and Mádl-Szonyi, J., 2012. Biofilm bacterial communities inhabiting the cave walls of the buda thermal karst system, hungary.

Geomicrobiology Journal, 29(7), pp. 611–627.

Chen, H., Li, D., Mao, Q., Xiao, K. and Wang, K., 2019. Resource limitation of soil microbes in karst ecosystems. Science of the Total Environment, 650, pp. 241–248.

Charousova, I., Medo, J., Hleba, L., Cisarova, M. and Sona, M., 2019. Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa. Brazilian Journal of Pharmaceutical Sciences, 55;el 17249, pp.1–11.

Chiaraphongphon, S., Suriyachadkun, C., Tamura, T. and

Thawai, C., 2010. Dactylosporangium maewongense sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 60(5), pp. 1200–1205.

Flora, D.O., Adeyemi, A.I. and George, W.P., 2015.

Hyoscyamine-producing marine Actinomycetes from Lagos Lagoon sediment. Asian Pacific Journal of Tropical Biomedicine, 5(3), pp. 196–201.

Franco-Correa, M., Quintana, A., Duque, C., Suarez, C., Rodríguez, M. X. and Barea, J.-M., 2010. Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Applied Soil Ecology, 45(3), pp. 209–217.

Gebreyohannes, G., Moges, F., Sahile, S. and Raja, N., 2013. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 3(6), pp. 426–435.

Goldscheider, N., 2019. A holistic approach to groundwater protection and ecosystem services in karst terrains. Carbonates and Evaporites, 34(4), pp. 1241–1249.

Goodfellow, M. and Fiedler, H.P., 2010. A guide to successful bioprospecting: Informed by actinobacterial systematics. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 98(2), pp. 119–142.

Hayakawa, M. and Nonomura, H., 1987. Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology, 65(5), pp. 501–509.

Hayakawa, M. and Nonomura, H., 1989. A New method for the intensive isolation of actinomycetes from soil. Actinomycetologica, 3(2), pp. 95–104.

Hayakawa, M., Sadakata, T., Kajiura, T. and Nonomura, H., 1991. New methods for the highly selective isolation of Micromonospora and Microbispora from soil. Journal of Fermentation and Bioengineering, 72(5), pp.320–326.

Hirsch, A. M. and Valdés, M., 2010. Micromonospora: An

important microbe for biomedicine and potentially for biocontrol and biofuels. Soil Biology and Biochemistry, 42(4), pp. 536–542.

Hu, N., Li, H., Tang, Z., Li, Z., Li, G., Jiang, Y. and Lou, Y., 2016. Community size, activity and C: N stoichiometry of soil microorganisms following reforestation in a Karst region. European Journal of Soil Biology, 73, pp.77–83.

Istianto, Y., Setyo, R., Koesoemowidodo, A., Saputra, H., Watanabe, Y., Pranamuda, H. and Marwoto, B., 2012. Short Communication Application of phenol pretreatment for the isolation of rare actinomycetes from Indonesian soil. Microbiology Indonesia, 6(1), pp. 42–47.

Jiang, Y., Li, Q., Chen, X. and Jiang, C., 2016. Isolation and Cultivation Methods of Actinobacteria. Actino-bacteria - Basics and Biotechnological Applications, pp. Chapter 2. pp 39–57. Intech Open Access Book. http://dx.doi.org/105772/61457

Kawuri, R. and Darmayasa, I.B.G., 2019. Bioactive compound from extract filtrat streptomyces sp.Sp1. as biocontrol of vibriosis on larvae of Macrobrachium rosenbergii shrimps. Hayati Journal of Biosciences, 26(1), pp.15–25.

Khamna, S., Yokota, A. and Lumyong, S., 2009. Actinomycetes isolated from medicinal plant rhizosphere soils: Diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production Actinomycetes isolated from medicinal plant

rhizosphere soils : diversity and screening of an. World Journal of Microbiology and Biotechnology, 25, pp. 649–655.

Kim, O., Cho, Y., Lee, K., Yoon, S., Kim, M., Na, H. and Chun, J., 2012. Introducing EzTaxon-e : a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62, pp. 716–721.

Kumar, P. S., Duraipandiyan, V. and Ignacimuthu, S., 2014. Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces. Kaohsiung Journal of Medical Sciences, 30(9), pp. 435–446.

Kumar, R.R., and Jadeja, V.J., 2016. Isolation of Actinomycetes: A Complete Approach. International Journal of Current Microbiology and Applied Sciences, 5(5), pp. 606–618.

Lian, B., Yuan, D.X. and Liu, Z.H., 2011. Effect of microbes on karstification in karst ecosystems. Chinese Science Bulletin, 56(35), pp. 3743–3747.

Moreno, D.A., García, A.M., Naffakh, M., Fernández, M.A., Paz, M. I., Montero, F. and Moreno, A., 2013. Cave Microbiomes: A Novel Resource for Drug Discovery, I (2001), pp 2–5.

Naikpatil, S.V. and Rathod, J.L., 2011. Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar. Journal of Ecobiotechnology, 3(10), pp. 48–53.

Niyomvong, N., Pathom-Aree, W., Thamchaipenet, A. and Duangmal, K., 2012. Actinomycetes from tropical limestone caves. Chiang Mai Journal of Science, 39(3), pp. 373–388.

Pronk, M., Goldscheider, N. and Zopfi, J., 2009. Microbial communities in karst groundwater and their potential use for biomonitoring. Hydrogeology Journal, 17(1), pp. 37–48.

Putri, A. L., Lisdiyanti, P. and Kusmiati, M., 2018. Identifikasi Aktinomisetes Sedimen Air Tawar Mamasa, Sulawesi Barat Dan Aktivitasnya Sebagai Antibakteri Dan Pelarut Fosfat. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(2), pp. 139–148.


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