THE EFFECT OF CHROMIUM STRESS ON MICRO-ANATOMICAL PROFILE OF CHILI (Capsicum annuum L.)

Nur Fitrianto, Siti Samiyarsih, Dede Winda Nur Fauziah, Sri Lestari
| Abstract views: 208 | PDF views: 382

Abstract

Chromium contamination can affect morphological, physiological, and anatomical changes, especially in chili vegetative organs. This research aims to understand micro-anatomical structure of the vegetative organs of chili subjected to chromium stress. The aim of the research is to know whether there is a micro-anatomical character difference between contaminated and uncontaminated chromium. The experimental methods using a completely randomized design (CRD). The independent variable is five levels of chromium concentration, i.e., 0; 50; 100; 200; and 300 ppm. The method of preparing anatomical preparations of the stem using a non-embedding and embedding method. The parameters observed in root and stem are organ diameter, xylem, thick of epidermis, and cortex. In contrast, leaf organ
parameter includes thick cuticle, epidermis, mesophyll, stomata size (length and width), and stomatal number. The research results showed that organ anatomical structure, i.e., root, stem, and leaf in chromium stress condition did not change. Chromium deposition causes a
decrease in root diameter, xylem, thick cortex roots, stem diameter, xylem, the thickness of the epidermis and cortex, leaf epidermal thickness, mesophyll, size of stomata width and number. In contrast, the chromium stress causes an increase in the thickness of the root epidermis (43,18%), cuticle thickness (36,36%), and leaf stomata length (33,33%) of chili as chromium concentrations increase. The anatomical structure of chili leaves changes after being contaminated chromium stress.

 

Keywords

Capsicum annuum, chromium, micro-anatomy, vegetative organ

Full Text:

PDF

References

Anugrah, T., 2014. Distribusi dan Bioakumulasi Krom Heksavalen Pada Tanaman Sorgum (Sorghum bicolor (L.) Moench). Skripsi. Salatiga: Universitas Kristen Satya Wacana.

Ardyanto, R. D., Santoso, S., and Samiyarsih, S. 2014. Kemampuan Tanaman Glodogan Polyalthia Longifolia Sonn. sebagai Peneduh Jalan dalam Mengakumulasi Pb Udara Berdasarkan Respon Anatomis Daun di Purwokerto. Scripta Biologica, (1), pp. 17-21.

Ariyanti, D., Johanes, D.B. and Fida, R., 2015. Analisis Struktur Daun Sawi Hijau (Brassica rapa var. Parachinensis) yang Dipapar dengan Logam Berat Kromium. Lentera Bio. 3(1), pp. 37-42.

Chandra, R.P., Abdussalam, A.k., Nabeesa, S. and Jos, T.P., 2010. Distribution of Bio-accumulated Cd and Cr in Two Vigna Species and The Associated Histological Variation. Journal of Stress Physiology and Biochemistry, 6(1), pp. 4-12.

Darmawan, A. B, 2012. Pengaruh penggunaan lumpur limbah industri penyamakan kulit terhadap penyerapan krom pada tanaman sawi. Majalah Kulit, Karet, dan Plastik, 28(2), pp. 69-78.

De Silva, N.D.G., Cholewa, E., and Ryser, P., 2012. Effects of Combined Drought and Heavy Metal Stresses on Xylem Structure and Hydraulic Conductivity in Red Maple (Acer rubrum L.). Journal of Experimental Botany, 1(1), pp. 1-10.

Dias, G.B., Gomes, V.M., Moraes, T., Zottich, U.P., Rabelo, G.R., Carvalho, A.O., Goncalves, L., Rodrigues, R., and Cunha, M.D., 2013. Characterization of Capsicum species Using Anatomical and Molecular Data. Genetics and Molecular Research, 12(4), pp. 6488-6501.

Farzadfar, S., & Zarinkamar, F., 2012. Morphological and Anatomical Response of Matricaria chamomilla Plants to Heavy Metal. Advance in Environmental Biology, 6(5), pp. 1603-1609.

Gomes, M.A.C., Rachel, H.D., Marina, S.S., and Angela, P.V., 2017. Plant Chromium Uptake and Transport, Physiological Effect, and Recent Advances in Molecular Investigations. Ecotoxicology and Environmental Safety, 1(40), pp. 55-64.

Gupta, K., Sumati, G., and Kumkum, M., 2011. Chromium accumulation in Submerged Aquatic Plants Treated With Tannery Effluent at Kanpur, India. Journal of Environmental Biology. 32(1), pp. 591-597.

Herawati W, Amurwanto A, Nafi’ah Z, Ningrum AM, Samiyarsih S., 2018. Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity. Biodiversitas 19(): pp. 119-125.

Juwarno, J., Sumarsono, S., and Samiyarsih, S. 2009. The Effects of nitrogen fertilizer dosages on anatomical characters of Ipomoea batatas L. leaf. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 26(1), pp. 30-34.

Juwarno, J., and Samiyarsih, S., 2017. Anatomical and Molecular Responses of Soy Bean (Glycine Max (L.) Merr.) Due to Salinity Stresses. Molekul, 12(1), pp. 45-52.

Lei, Y., Chen, K., Tian, X., and Korpelainen, H., 2007. Effect of Heavy Metal Toxicity on Morphological and Physiological Changes in Two Populus Cathayana Populations Originating from Different Habitats. Trees, 1(21), pp. 569-580.

Kasmiyati, S., 2016. Mekanisme Toleransi Sorghum bicolor (L.) Moench. Terhadap Cekaman Krom: Kajian Fisiologis, Anatomis, Biokimia dan Molekular. Dissertation. Universitas Gadjah Mada, Yogyakarta. Indonesia.

Kasmiyati, S. and Sucahyo, 2014. Deteksi Cekaman Oksidatif Akibat Toksisitas Krom Pada Sanchus oleraceus Melalui Penentuan Spesies Oksigen Reaktif Secara Spektrofotometri dan Histokimia. Agriculture, 26(1), pp. 85-98.

Meuser, H., 2012. Soil Decontamination. Soil Remediation and Rehabilitation Environmental Pollution. 23: 201-278

Rahardjo, S. M., 2014. Struktur Anatomi Organ Vegetatif Sorghum bicolor L. Pada Kondisi Cekaman Krom. Skripsi. Salatiga: Universitas Kristen Satya Wacana.

Samiyarsih S, Naipospos N, Palupi D., 2019. Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas 20(10) pp. 2986-2993.

Samiyarsih S, Fitrianto N, Azizah E, Herawati W, Rochmatino, 2020. Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers. Biodiversitas 21(4) pp. 1755-1766.

Samiyarsih, S., Suparjana, T.B., and Juwarno, 2016. Karakter Anatomi daun Tumbuhan Mangrove Akibat Pencemaran di Hutan Mangrove Kabupaten Cilacap. Biosfera, 33(1), pp. 31-36.

Santoso, S., Lestari, S an Samiyarsih, S., 2012. “Inventarisasi Tanaman Peneduh Jalan Penjerap Timbal di Purwokerto,” Semin. Berkelanjutan II, no. 2004, pp. 197–203

Tupan, C., I. & Rodiyati, A., 2016. Accumulation and deposition of lead-heavy metal in the tissues of roots, rhizomes, and leaves of seagrass Thalassia hemprichii (Monocotyl, Hydrocharitaceae). AACL Bioflux, 9(3), pp. 580-589.

Vazquez, MD., Poschenrieder, CH., and Barcelo, J., 1987. Chromium VI Induced Structural and Ultrastructural Changes in Bush Bean Plants (Phaseolus vulgaris L.). Annals of Botany, 1(59), pp. 427-438.


Refbacks

  • There are currently no refbacks.