JUMLAH, UJI VIABILITAS DAN DAYA KECAMBAH POLEN 31 AKSESI PISANG (Musa sp.) KOLEKSI KEBUN PLASMA NUTFAH PISANG LIPI

Erwin Fajar Hasrianda, Ahmad Zaelani, Yuyu Suryasari Poerba
10.14203/beritabiologi.v19i2.3859
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Abstract

Cross pollination is one of the plant breeding techniques to create a better cultivar with desired traits. A successful cross pollination is affected by quality and quantity of the pollen used. The aim from this study is to know amounts, viability and germination from various banana accessions. This study was conducted by using 31 banana accessions. Pollen quantity per anther, pollen viability, pollen germination growth were measured. The results showed that pollen amounts were ranged between 5.032 and 118.181 pollens per anther. In addition, pollen viability ranged from 30% in Tingalun accession (Musa sp. cv. Tingalun)  to 99% in Malaccensis 2x accession. Furthermore, pollen germination level varied from 1% in Mambee Thu OS accession to 48% in Rutilifes 2x accession. We also found that negative correlation between pollen viability and pollen germination were moderate with correlation coeeficient value of -0,59 ( p-value = 0,00051). From 31 banana accessions tested in this research, pollen source from Malaccensis 2x and Rutilifes 2x banana accessions have the highest potential to be used as the male parent.

 

Keywords

Amounts, 31 Banana accessions, Pollen germination, Pollination, Pollen, Viability

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References

Brewbaker, J.L. and Kwack, B.H., 1963. The essential role of calcium ion in pollen germination and tube growth. American Journal of Botany. 50(9), pp. 747–858.

Chandler, S., 1995. The nutritional value of bananas. di dalam: Gowan S, editor. Bananas and Plantains. London (GB): Chapman and Hall.

FAOSTAT. Food and Agriculture Organization of The United Nations. 2015. World Agriculture Towards 2015/ 2030. An FAO Perspective. http://www.fao.org/docrep/ 005/y4252e/ y4252e05e.html. (diakses 25 Juli 2019)

Gibernau, M., Macquart, D. and Diaz, A., 2003. Pollen viability and longevity in two species of Arum italicum and A. maculatum. Aroideana, 26, pp.58–62.

Jenny C., Auboiron, E. and Beveraggi, A., 1994. Breeding plantain-type hybrids at CRBP. Pp. 176-187 in The improvement and testing of Musa: a global partnership (D.R. Jones, ed.). Proceedings of the first global conference of the IMTP held at FHIA, Honduras, 27-30 April 1994. INIBAP, Montpellier, France.

Kaemmar, D., Fischer, D., Jarret, R.L., Baurens, F.C., Grapin, A., Dambler, D., Noyer, J.L., Lanaud, C., Khald, G. dan Lagoda, P.J.L., 1997. Molecular breeding in the genus Musa: A strong case for STMC, marker technologi. Euphytica, 96, pp. 49–63.

Kelly, J.K., Rasch, A. and Kalisz, S., 2002. A method to estimate pollen viability frompollen size variation. American Journal of Botany, 89(6), 1021–1023.

Pillay, M.A. and Tenkuano, A., 2011. Banana Breeding Progress and Challenges. London (GB): CRC Pr.

Poerba, Y.S., D. Martanti, F. Ahmad, Herlina, T. Handayani, dan Witjaksono. 2018. Deskripsi Pisang-Koleksi Pusat Penelitian Biologi LIPI. LIP Press. Jakarta.

Rachman, E., Poerba, Y. S. dan Ahmad, F. 2012. Penyimpanan Serbuksari Pisang Liar Musa acuminata Colla Untuk Mendukung Program Pemuliaan Pisang Budidaya. Berita Biologi, 11(2), 167–175.

Shivanna, K.R. and Rangaswamy, N.S., 1992. Pollen Biology A Laboratory Manual. Berlin (BE): Springer-Verlag.

Soares, T.L., Everton, H.S., Maria, A.P.C.C., Sebastião, O.S. and Janay, A.S.S., 2016. Viability of pollen grains of tetraploid banana. Bragantia, 75(2), pp. 145–151.

Swennen, R. and Vuylsteke, D., 1990. Aspects of plantain breeding at IITA. Pp. 252–266 In: Sigatoka leaf spot disease of bananas (R.A. Fullerton and R.H. Stover, eds). Proceedings of an international workshopheld at San José, Costa Rica, 28 March-1 April 1989. INIBAP, Montpellier, France.

Tenkouano, A., Crouch, J.H., Crouch, H.K. and Vuylsteke, D., 1989. Ploidy determination in Musa germplasm using pollen and chloroplast characteristics. Hortsci, 33(5), pp. 889–890.

Tomekpé, K., Noupadja, N., Abadie, C., Auboiron, E. and Tchango Tchango, J., 1998. Genetic improvement of plantains at CRBP: performance of black Sigatoka resistant plantain hybrids. Pp. 45–50 In: Actas: Seminario Internacional sobre Producción de Plátano.

-8 de Mayo 1998, Armenia, Quindío. CORPOICA, Colombia.

Valmayor, R.V., Jamaluddin, S.H., Silayoi, B., Kusumo, S., Danh, L.D., Pascua, O.C. and Espino, R.R.C., 2000. Banana Cultivar Names and Synonyms in Southeast Asia. Los Banos (PH): INIBAP.

Warid dan Palupi, E.R., 2009. Korelasi Metode Pengecambahan IN VITRO dan pewarnaan dalam pengujian viabilitas polen. Prosiding Seminar Departeman Agronomi dan Hortikultura. Institut Pertanian Bogor.

Welsiliana. 2018. Karakterisasi dan Viabilitas Polen Pisang Liar dan Pisang Budidaya. Thesis. Institut Pertanian Bogor. Bogor.

DOI: https://doi.org/10.14203/beritabiologi.v19i2.3859

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