ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG

Prilya Dewi Fitriasari, Nanda Amalia, Susiyanti Farkhiyah
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Abstract

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.

 

 

 

Keywords

Bacteria, Compatibility, Consortium, Enzymes

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References

Anand, A.A.P., Vennison, S.J., Sankar, S.G., Prabhu, D.I.G., Vasan, P.T., Raghuraman, T., Geoffrey, C.J. and Vendan, S.E., 2009. Isolation and Characterization of Bacteria from the Gut of Bombyx Mori that Degrade Cellulose, Xylan, Pectin and Starch and Their Impact on Digestion. Journal of Insect Science, 10(107), pp. 1–20.

Hibbing, M.E., Fuqua, C., Parsek, M.R. and Peterson, S.B., 2010. Bacterial competition: Surviving and thriving in the microbial jungle. Nature Review Microbiology, 8(1), pp.15–25.

Indumathi, D., 2017. Microbial conversion of vegetable wastes for bio fertilizer production. IOSR- Journal of Biotechnology and Biochemistry, 3(2), pp.43–47.

Murtiyaningsih, H. dan Hazmi, M., 2017. Isolasi dan Uji Aktivitas Enzim Selulase pada Bakteri Selulolitik Asal Tanah Sampah. Agritrop (Jurnal Ilmu-Ilmu Pertanian), 15(2), pp. 293–308.

Raju, E.V. and Divakar, G., 2013. Production of pectinase by using Bacillus circulans isolated from dump yards of vegetable wastes. International Journal of Pharmaceutical Sciences Research, 4(7), pp. 2615–2622.

Saha, A. and Santra, S.C., 2014. Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. Journal of Microbiology & Experimentation, 1(1), pp.1–8.

Saraswati, R., Santosa, E. dan Yuniarti, E., 2006. Organisme Perombak Bahan Organik. Pupuk Organik dan Pupuk Hayati. Buku pupuk Organik dan Pupuk Hayati. Pp. 211–215. Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian. Bogor.

Sarkar, P. and Chourasia R., 2017. Bioconversion of organic solid wastes into biofortified compost using a microbial consortium. International Journal of Recycling of Organic Waste in Agriculture. 6, pp. 321–334.

Sharma, A.K., Sharma, V., Saxena, J., Chandra, R., Alam, A. and Prakash, A. 2015. Isolation and screening of amylolytic bacteria from soil. International Journal of Scientific Research in Agricultural Sciences, 2(7), pp. 159–165.

Shu-Hsien, T., Ching-Piao, L., Shang-Shyng, Y., 2007. Microbial Conversion of Food Wastes for Biofertilizer Production with Thermophilic Lipolytic Microbes. Renewable energy, 32, pp. 904–915.


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