AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK IXORA CUMINGIANA

Kartika Dyah Palupi, Praptiwi Praptiwi, Dewi Wulansari, Andria Agusta
| Abstract views: 224 | Pdf views: 1259

Abstract

Plants from Ixora genus exhibit a variety of pharmacological activities including antioxidant, antibacterial, and antitumor activities. This plants may contain many interesting bioactive compounds, especially phenolics and terpenoids groups. Ixora cumingiana is one of the Ixora species whose pharmacological effect has not been explored comprehensively. This study aimed to evaluate the antibacterial and antioxidant activity of eight extracts from bark and leaf of I. cumingiana. The plant samples were successively extracted using n-hexane, dichloromethane, ethyl acetate and methanol. The antibacterial evaluation was carried out against Staphylococcus aureus and the antioxidant activity was evaluated using a radical scavenging assay against 1,1-diphenyl-2-picrylhydrazyl (DPPH). Initial activity screening was performed using thin layer chromatography-bioautography followed by the determination of minimum inhibitory concentration (MIC) and the determination of antioxidant activity index (AAI) using microdilution technique. The dichloromethane extract of the bark as well as the n-hexane and dichloromethane extract of the leaf of I. cumingiana exhibited moderate antibacterial effect with MIC value of 128, 128, and 256 µg/ml, respectively. The methanol extract from the bark displayed a strong antioxidant activity (AAI = 1.5±0.13) and possessed the highest total phenolic content (43±0.91 mg AGE/ g extract). These experimental results showed that I. cumingiana is potential to be developed as an antioxidant agent rather than as an antibacterial agent.
 

Keywords

Ixora cumingiana, antioxidant activity, antibacterial activity, Staphylococcus aureus, DPPH

Full Text:

Pdf

References

Annapurna, J., Amarnath, P. V. S., Amar Kumar, D., Ramakrishna, S. V. and Raghavan, K. V., 2003. Antimicrobial activity of Ixora coccinea leaves. Fitoterapia, 74(3), pp. 291–293. https://www.sciencedirect.com/science/article/abs/pii/S0367326X03000376 (diakses 27 Juni 2019)

Buathong, R., Chamchumroon, V., Schinnerl, J., Bacher, M., Santimaleeworagun, W., Kraichak, E. and Vajrodaya, S., 2019. Chemovariation and antibacterial activity of extracts and isolated compounds from species of Ixora and Greenea (Ixoroideae,

Rubiaceae). PeerJ, 7, pp. 1–14. https://www.ncbi.nlm.nih.gov/pubmed/31119085 (diakses 16 Juli 2019)

Chamchuroon, V., 2006. A checklist of the genus Ixora L. (Rubiaceae) in Thailand. Thai Forest Bulletin (Botany), 34, pp. 4–24. https://www.tci-thaijo.org/index.php/ThaiForestBulletin/article/view/24211 (diakses 4 Juli 2019)

Chen, L.-J., Zhang, Y. and Chen, Y.-G., 2016. Chemical Constituents of Plants from the Genus Ixora. Chemistry & Biodiversity, 13(3), pp. 275–283. https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.201500065 (diakses 10 Juli 2019)

Chen, R., Li, P., Huo, L., Lu, R., Lu, C. and Su, W., 2013. Antioxidant activity of Ixora chinensis. Asian Journal of Chemistry, 25(4), pp. 2323–2324. http://www.asianjournalofchemistry.co.in/User/ViewFreeArticle.aspx?ArticleID=25_4_129 (diakses 10 Juli 2019)

Jaiswal, R., Karar, M. G. E., Gadir, H. A. and Kuhnert, N., 2014. Identification and characterisation of phenolics from Ixora coccinea L. (Rubiaceae) by Liquid Chromatography Multi-stage Mass Spectrometry. Phytochemical Analysis, 25(6), pp. 567–576. John Wiley & Sons, Ltd. https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.2530 (diakses 20 Juni 2019)

Kan, N.-W., Huang, W.-C., Lin, W.-T., Huang, C.-Y., Wen,K.-C., Chiang, H.-M., Huang, C.-C., et al., 2013. Hepatoprotective effects of Ixora parviflora extract against exhaustive exercise-induced oxidative stress in mice. Molecules, 18(9), pp. 10721.Multidisciplinary Digital Publishing Institute (MDPI). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269953/ (diakses 31 Juli 2019)

Lee, C.-L., Liao, Y.-C., Hwang, T.-L., Wu, C.-C., Chang, F.-R. and Wu, Y.-C., 2010. Ixorapeptide I and ixorapeptide II, bioactive peptides isolated from Ixora coccinea. Bioorganic & Medicinal Chemistry Letters, 20(24), pp. 7354–7357. https://www.sciencedirect.com/science/article/pii/S0960894X10015192?via%3Dihub (diakses 25 Juli 2019)

Mouly, A., Razafimandimbison, S. G., Khodabandeh, A. and Bremer, B., 2009. Phylogeny and classification of the species-rich pantropical showy genus Ixora (Rubiaceae-Ixoreae) with indications of geographical monophyletic units and hybrids. American Journal of Botany, 96(3), pp. 686–706. https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/ajb.0800235 (diakses 28 Juni 2019)

Nair, S.C. and Panikkar, K. R., 1990. Antitu mour principles from Ixora javanica. Cancer Letters, 49(2), pp. 121–126. Elsevier. https://www.sciencedirect.com/science/article/abs/pii/030438359090147P (diakses 25 Juni 2019)

Pessini, G. L., Dias Filho, B. P., Nakamura, C. V. and Cortez, D. A. G., 2003. Antibacterial activity of extracts and neolignans from Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck. Memorias do Instituto Oswaldo Cruz, 98(8), pp. 1115–1120. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800025&lng=en&nrm=iso&tlng=en (diakses 1 Februari 2019)

Poojari, M., Padyana, S. and Rao, B. R., 2009. Evaluation of Antioxidant and Antimicrobial Properties of Ixora brachiata Roxb. E-Journal of Chemistry, 6(3), pp. 625–628. https://www.hindawi.com/journals/jchem/2009/962753/abs/ (diakses 25 Juni 2019)

Ragasa, C. Y., Tiu, F. and Rideout, J. A., 2004. New Cycloartenol Esters from Ixora Coccinea. Natural Product Research, 18(4), pp. 319–323. https://www.tandfonline.com/doi/abs/10.1080/14786410310001630519 (diakses 26 Juni 2019)

Ratnasooriya, W. D., Deraniyagala, S. A., Galhena, G., Liyanage, S. S. P., Bathige, S. D. N. K. and Jayakody, J. R. A. C., 2005. Anti-inflammatory activity of the aqueous leaf extract of Ixora coccinea. Pharmaceutical Biology, 43(2), pp. 147–152. https://www.tandfonline.com/doi/full/10.1080/13880200590919483 (diakses 24 Juni 2019)

Sadeghi-Nejad, B. and Deokule, S., 2009. Antidermatophytic activities of Ixora brachiata Roxb. African Journal of Biochemisty Research, 3(10), pp. 344–348.

Saha, M. R., Alam, M. A., Akter, R. and Jahangir, R., 2008. In vitro free radical scavenging activity of Ixora coccinea L. Bangladesh Journal of Pharmacology, 3(2).https://www.banglajol.info/index.php/BJP/article/view/838 (diakses 7 Juni 2019)

Scherer, R. and Godoy, H. T., 2009. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chemistry, 112(3), pp. 654–658. https://www.sciencedirect.com/science/article/pii/S0308814608007218 (diakses 11 Januari 2019)

Torey, A., Sasidharan, S., Latha, L. Y., Sudhakaran, S. and Ramanathan, S., 2010. Antioxidant activity and total phenolic content of methanol extracts of Ixora coccinea. Pharmaceutical Biology, 48(10), pp. 1119–1123.https://www.tandfonline.com/doi/full/3109/13880200903490505 (diakses 12 April 2019)

Tosh, J., Dessein, S., Buerki, S., Groeninckx, I., Mouly, A., Bremer, B., Smets, E. F., et al., 2013. Evolutionary history of the Afro-Madagascan Ixora species (Rubiaceae): species diversification and distribution of key morphological traits inferred from dated molecular phylogenetic trees. Annals of botany, 112(9), pp. 1723–1742. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838549/ (diakses 12 Juni 2019)

Usha, M., Reginald Appavoo, M., and Immanue, G., 2012. Pharmacognostical study and preliminary phytochemical screening of the leaf of Ixora johnsonii Hook.f. - A rare, endemic, critically endangered species of Southern Western Ghats of Kerala, India. International Journal of Pharmaceutical and Phytopharmacological Research, 1(5), pp. 263–270. https://eijppr.com/en/article/pharmacognostical-study-and-preliminary-phytochemical-screening-of-the-leaf-of-ixora-johnsonii-hookf-a-rare-endemic-critically-endangered-species-of-southern-western-ghats-of-keral. (diakses 23 Juli 2019)

Usha, M., Reginald Appavoo, M. and Immanue, G., 2016. Ixora L . - an Overview. European Journal of Pharmaceutical and Medical Research, 3(2), pp. 146–154

https://www.ejpmr.com/admin/assets/article_issue/1454130284.pdf (diakses 23 Juli 2019)


Refbacks

  • There are currently no refbacks.