SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT
Oscar Efendy, Ahmad Fathoni, Praptiwi Praptiwi, Mohammad Fathi Royyani, Dewi Wulansari, Andria Agusta
| Abstract views: 842
| 
PDF views: 2614
Abstract
Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml. Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.
Keywords
Full Text:
PDFReferences
Beveridge, T.J., 1999. Structures of gram-negative cell walls and their derived membrane vesicles. Journal of Bacteriology. 181(16), pp.4725–4733.
Bolton, B., 1994. Identification Guide to the Ant Genera of the World. Havard University Press. London.
Brütsch, T., Jaffuel, G.,Vallat, A., Turlings, T.C.J. and Chapuisat, M. 2017. Wood ants produce a potent antimicrobial agent by applyingformic acid on tree-collected resin. Ecology and Evolution. 7(7), pp. 2249–2254.
Costa, Nesto E.M., 1999. Traditional Use and Sale of Animals as Medicine in Fiera de Santana City, Bahia, Brazil. Indigenous Know Dev Monitor.
Davila, F., Botteaux, A., Bauman, D., Cherasse, S. and Aron, S., 2018. Antibacterial activity of male and female sperm-storage organs in ants. Journal of Experimental Biology, 221(Pt 6):jeb.175158. DOI: 10.1242/jeb.175158
Dewanjee, S., Gangopadhyay, M., Bhattacharya, N., Khanra, R., Tarun K Dua., 2015. Bioautography and its scope in the field of natural product chemistry. Journal of Pharmaceutical Analysis, 5(2), pp. 75-84. https://doi.org/10.1016/j.jpha.2014.06.002
Durst, P.B., Johnson, D.V., Leslie, R.N. and Shono, K., 2010. Edible Forest Insect: Human Bite Back. FAO RAP Publication 2010/02. Bangkok. Thailand.
Huis, A.V., Itterbeeck, J.V., Klunder, H., Mertens, E., Halloran, A.,Muir, G., and Vantomme, P., 2013. Edible insect: Future Prospect for food and feed security. FAO Forestry Paper. FAO. Rome.
Kouřimská, L. and Adámková, A., 2016. Review article Nutritional and sensory quality of edible insects. NFS Journal 4, pp. 22–26.
Kuete, V., Kruche, B., Youns, M., Voukeng, I., Fankam, A.G., Tankeu, S. et al., 2011. Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts. Journal of Ethnopharmacology, 1034, pp. 803–812.
Melo-Ruiz, V., Quirino-Barreda, T., Calvo-Carrillo, C., Sánchez-Herrera, K. and Sandoval-Trujillo, H., 2013. Assessment of Nutrients of Escamoles Ant Eggs Limotepum apiculatum M. by Spectroscopy Methods. Journal of Chemistry and Chemical Engineering, 7, pp. 1181–1187.
Oladunmoye, M.K., Akinmaye, A.S. and Oladosu, T.O., 2018. Antimicrobial activity of abdominal gland extracts of African Weaver Ants (Oecophylla longinoda) on isolated microorganisms from kola nut pods. Acta Scientific Microbiology, 1(2), pp. 09–14.
Padmanabhan, P., Gopalakrishnani, R. and Kasinathan K., 2012. Antibacterial eficiency in the hemolymph of Black Ant, Camponotus compressus. International Journal of Pharma and Bio Sciences, 3(1), pp. B-503 – B-506.
Rabeling, C., Brown, J.M. and Verhaagh, M., 2008. New discovered sister lineage sheds light on early ant evolution. Proceedings of the National Academy of Sciences of the United States of America, 105, p. 14913.
Samakovlis, C., Kylsten, P., Kimbrell, D.A., Engstrom, A. and Hultmark, D., 1991. The andropin gene and its product, a male-specific antibacterial peptide in Drosophila melanogaster. EMBO Journal. 10, pp.163–169.
Shahverdi, A.R., Abdolpour, F., Monsef-Esfahani, H.R. and Farsam, H., 2007. A TLC bioautographic assay for the detection of nitrofurantoin resistance reversal compound. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 850 (1-2), pp. 528–530.
Silva, M.T.G., Simas, S.M., Batista, T.G.F.M., 2005. Studies on antimicrobial activity, in vitro, of Physalis angulata L. (Solanaceae) fraction and physalin B bringing out the importance of assay determination. Memórias do Instituto Oswaldo Cruz, 100, pp.779–782.
Siva-Jothy, M.T., Moret, B.Y. and Rolffa, J., 2005. Insect Immunity: An Evolutionary Ecology Perspective. Advances in Insect Physiology, Vol. 32, ISBN 0-12-024232-X, Elsevier Ltd.
Solis, M. A., 1999. Insect biodiversity: Perspectives from the systematist. American Entomology. 45 (4), pp. 204–205.
Wilsanand, V., Varghese, P. and Rajitha, P., 2007. Therapeutics of insect and insect products in South Indian traditional medicine. Indian Journal of Traditional Knowledge, 6(4), pp. 563–568.
Valle Jr. D.L., Puzon, J.JM., Cabrera, E.C. and Rivera, W.L., 2016. Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from Philippine Piper betle L. against Multidrug-Resistant Bacteria. Evidence-Based Complementary and Alternative Medicine, Volume 2016, Article ID 4976791, http:// dx.doi.org/10.1155/2016/4976791.
Refbacks
- There are currently no refbacks.