INDUKSI POLIPLOIDI DENGAN KOLKISIN PADA HIBRID F1 HASIL PERSILANGAN ANTAR SPECIES PADA TANAMAN PANILI ASAL CIAMIS

Fitri Damayanti, Ika Mariska
| Abstract views: 718 | PDF views: 470

Abstract

Steem root disease caused by Fusarium oxysporum Schl. f. sp. Vanillae (Tucker) Gordon represent one internal issues of vanilla development (Vanilla planifolia Andrews). To obtain resistance clone to the disease, it can exploit resource of wild vanilla (V. albida B. L Syn) through crosses. Hybrids which were interspecific crossed generally were sterile. To overcome sterility problem of the hybrids, chromosome doubling was made by colchicine application. Explant used globular structure of proembryo from F1 seed result from a cross between wild vanilla of Ciamis as female parents and cultivated vanilla clone of Ciamis as male parent. Concentration level colchicine used were 0.00%, 0.05%, 0.10%, 0.20% and 0.25% with period of treatment of 3 and 6 days. After colchicine treatment embryo cultures were subcultured into new mwdium that was basal media Murashige-Skoog enriched with 2.5 mg/l BAP. Result of the experiment showed that colchicine treatment,globular structure were F1 embryo tending to inhibit early regeneration. The cultures showed variabilities from treatment of colchicin 0.20% during of 6 day and 0.25% for 3 days. Phenotypic performance of the chromosome doubled hybrids showing great variation in color and vigor of the culture. Tetraploid plant(2n=4x=64) was obtained from the colchicine treatment of 0.25% for 6 days. Chromosome addtion was followed by improvement of cell dimensions and organ magnification.

Keywords

polyploidy, Vanilla planifolia (Andrews), Vanilla albida (B.L. Syn), colchicine.

Full Text:

PDF

References

Mariska I,Crowder LV. 1990. Genetika Tumbuhan. Gadjah Mada University Press. Yogyakarta. Hlm 297.

Darlington CD and Wylie AP. 1955. Chromosome Atlas of Flowering Plants. University Press Aberdeen. London. Hlm 405.

Darnaedi D. 1990. Training Teknik Sitologi Angkatan 1. Herbarium Bogoriensis. Balitbang Botani. Puslitbang Biologi LIPI, 1-10.

Eigisti PJ and Dustin PJ. 1957. Colchicine in Agriculture, Medicine Biology and Chemistry. The Lowa State Collage Press. Lowa. Hlm 275.

Hasan L, Jones RN, Parker JS and Posselt UK. 1991. Colchicine inducted heritable variation in cell size and chloroplast number in the leaf cells of inbredrye grasses (Lolium perenne, L. multiforum). Euphytica 52, 39-45.

Husni A, Sukmadaja D dan Mariska I. 1995. Variasi somaklonal tanaman panili dengan mutagen kimia kolkisin secara in vitro. Prosiding Evalusai dan Hasil Penelitian Tanaman Industri. Puslitbangtan, 8-16.

King RC. 1965. Genetics. Oxford University Press. Oxford. Hlm 170-171.

Linsey K and Jones MGK. 1989. Plant Biotechnology in Agriculture. Open University Press Milton Keynes. Hlm 9.

Mariska I, Seswita D dan Purnamaningsih R. 1995. Peningkatan keragaman genetik tanaman nilam dengan kolkisin. Prosiding Evaluasi Hasil Pertanian Tanaman Industri Buku I. puslitbangtan, 34-39.

Mariska I, Hobir, Husni A, Kosmiatin M dan Rusyadi Y. 1996. Penyelamatan embria hasil persilangan antara panili budidaya dan panili liar. Balitbio, 1-5(Unpublished).

Murashige T and Skoog F. 1962. A Revised mediu, for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15,473-497.

Nuryani Y, Tombe M dan Mogi S. 1996. Rspon beberapa tipe panila terhadap patogen busuk batang (Fusarium oxysporum F. sp. Vanillae). Proc. Seminar on Integrited Control on Main Disease if Industrial Crops. Puslitbangtan, 123-128.


Refbacks

  • There are currently no refbacks.