ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT ASAL AKAR TANAMAN KUNYIT (Curcuma longa) SEBAGAI ANTIMALARIA

Eris Septiana, Fauzy Rachman, Sylvia J.R. Lekatompessy, Harmastini I. Sukiman, Partomuan Simanjuntak
10.14203/beritabiologi.v17i3.3408
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Abstract

Malaria is still the leading cause of death worldwide with nearly half the world's population at risk. Parasitic resistance to existing antimalarial drugs in the market makes the search for a source of new drugs from nature is very important. Therefore, the aims of this study are to determine in vitro antimalarial activity of endophytic fungi extract from turmeric root and to identify the selected isolate molecularly. Heme polymerization inhibition method was used as in vitro antimalarial assay. The selected isolate was thrn identified using ITS1, ITS2, and 5.8S sequences of rDNA. The result of this study obtained 16 isolates of endophytic fungi from root of turmeric plant with isolate code were of K.Cl.Sb.A1 - K.Cl.Sb.A16. All of the ethyl acetate extracts of isolated endophytic fungi have heme polymerization inhibition activity. K.Cl.Sb.A11 was the most active isolate on heme polymerization inhibition test with 94,31% at concentration of test material at 8 mg/mL and IC50 value at 1.84 mg/mL. Molecular analysis showed that K.Cl.Sb.A11 isolate was Penicillium sp. and potentially developed as an antimalarial drug.

Keywords

Curcuma longa, endophytic fungi, Penicillium sp., heme polymerization, molecular identification

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References

Baelsmans, R., Deharo, E., Munoz, V., Sauvain, M. and Ginsburg, H., 2000. Experimental condition for testing the inhibitory acitivity of chloroquine on the formation of β-hematin. Experimental Parasitology, 96(4), pp. 243–248.

Basilico, N., Pagani, E., Monti, D., Olliaro, P. and Taramelli, D., 1998. A microtitre-based method for measuring the haem polymerization inhibitory activity (HPIA) of antimalarial drugs. Journal of Antimicrobial Chemotherapy, 42(1), pp. 55–60.

Bustanussalam, Rachman, F., Septiana, E., Lekatompessy, S.J.R., Widowati, T., Sukiman, H.I. and Simanjuntak, P., 2015. Screening for endophytic fungi from turmeric plant (Curcuma longa L.) of Sukabumi and Cibinong with potency as antioxidant compounds producer. Pakistan Journal of Biological Sciences, 18 (1), pp. 42–45.

Chenniappan, K. and Kadarkarai, M., 2010. In vitro antimalarial activity of traditionally used western ghats plants from India and their interactions with chloroquine against chloroquine-resistant Plasmodium falciparum. Parasitology Research, 107(6), pp. 1351–1364.

Cui, L., Mao, J. and Cui, L., 2007. Cytotoxic effect of curcumin on malaria parasite Plamodium falciparum: inhibition of histone acetylation and generation of reactive oxygen species. Antimicrobial Agents and Chemotherapy, 51(2), pp. 488–494.

Dai, C.C., Yu, B.Y. and Li, X., 2008. Screening of endophytic fungi that promote the growth of Euphorbia pekinensis. African Journal of Biotechnology, 7(19), pp. 3505–3510.

Darmawan, A. dan Lipinwati., 2014. Gambaran obat tradisional yang digunakan penderita malaria di wilayah Puskesmas Simpang IV Sipin Kota Jambi 2014. Jambi Medical Journal, 2(2), pp. 114–126.

Dompeipen, E.J. dan Simanjuntak, P., 2015. Aktivitas antidiabetes dan antioksidan kapang endofit dari tanaman mahoni (Swietenia macrophylla King). Biopropal Industri, 6(1), pp. 7–17.

Harborne, J.B., 1998. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. Chapman & Hall, England.

Hiraishi, A., Kamagata, Y. and Nakamura, N., 1995. Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens. Journal of Fermentation Bioengineering, 79(6), pp. 523–529.

Huy, N.T., Uyen, D.T., Maeda, A., Oida, T., Harada, S. and Kamei, K., 2007. Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds. Antimicrobial Agents and Chemotherapy, 51(1), pp. 350–353.

Jiazhong, L., Li, S., Bai, C., Liu, H. and Gramatica, P., 2013. Structural requirements of 3-carboxyl-4(1H)-quinolones as potential antimalarials from 2D and 3D QSAR analysis. Journal of Molecular Graphics and Modelling, 44, pp. 266–277.

Kaushik, N.K., Murali, T.S., Sahal, D. and Suryanarayanan, T.S., 2014. A search for antiplasmodial metabolites among fungal endophytes of terrestrial and marine plants of Southern India. Acta Parasitologica, 59(4), pp. 745–757.

Kumala, S. dan Pratiwi, A.P., 2014. Efek antimikroba dari kapang endofit ranting tanaman biduri. Jurnal Farmasi Indonesia, 7(2), pp. 111–120.

Martinez-Correa, H.A., Paula, J.T., Kayano, A.C.A.V., Queiroga, C.L., Magalhaes, P.M., Costa, F.T.M. and Cabral, F.A., 2017. Composition and antimalarial activity of extracts of Curcuma longa L. obtained by a combination of extraction processes using supercritical CO2 ethanol and water as solvents. The Journal of Supercritical Fluids, 119, pp. 122–129.

Muharni, Fitriya, Ruliza, M.O., Susanti, D.A. and Elfita., 2014. Di(2-Ethylhexyl(phthalate and pyranon derivated from endophytic fungi Penicillium sp the leave of kunyit putih (Curcuma zedoaria). Indonesian Journal of Chemistry, 14(3), pp. 290–296.

Murray, C.J.L., Rosenfeld, L.C., Lim, S.S., Andrews, K.G., Foreman, K.J., Haring, D., Fullman, N., Naghavi, M., Lozano, R. and Lopez, A.D., 2012. Global malaria mortality between 1980-2010: a systematic analysis. Lancet, 379(9814), pp. 413–431.

Nagendrappa, P.B., Naik, M.P. and Payyappallimana, U., 2013. Ethnobotanical survey of malaria prophylactic remedies in Odisha, India. Journal of Ethnopharmacology, 146(3), pp. 768–772.

Olorunnisola, O.S., Adetutu, A., Balogun, E.A. and Afolayan, A.J., 2013. Ethnobotanical survey of medicinal plants used in the treatment of malarial in Ogbomoso, Southwest Nigeria. Journal of Ethnopharmacology, 150(1), pp. 71–78.

Purwantini, I., Wahyono, Mustofa dan Susidarti, R.A., 2010. Aktivitas Inhibitor Polimerisasi Hem Metabolit Penicillium sp. Fungi Endofit yang Diisolasi dari Artemisia annua. Prosiding Seminar Nasional Eight Star Performance Pharmacist. Fakultas Farmasi Universitas Gadjah Mada, 1(1), pp. 155–159.

Purwantini, I., Wahyono, Mustofa dan Susidarti, R.A., 2015. Pengaruh media pada pertumbuhan fungi endofit IP-2 dan produksi metabolit aktif inhibitor polimerisasi hem. Traditional Medicine Journal, 20(1), pp. 51–56.

Putra, I.P., Rahayu, G. and Hidayat, I., 2015. Impact of domestication on the endophytic fungal diversity associated with wild Zingiberaceae at mount Halimun Salak National Park. HAYATI Journal of Biosciences, 22(4), pp. 157–162.

Sambrook, J. and Russel, D.W., 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Pr, USA.

Septiana, E., Bustanussalam, Rachman, F., Hapsari, Y. dan Simanjuntak, P., 2017. Potensi ekstrak kapang endofit asal rimpang kunyit sebagai antimalaria dan antioksidan. Jurnal Kefarmasian Indonesia, 7(1), pp. 1–9.

Theantana, T., Hyde, K.D. and Lumyong, S., 2007. Asparaginase production by endophytic fungi isolated from some Thai medicinal plants. KMITL Science and Technology Journal, 7(1), pp. 13–18.

Tomita, F., 2003. Endophytes in Southeast Asia and Japan: their taxonomic diversity and potential applications. Fungal Diversity, 14, pp. 187–204.

Wahyono, Pudjiono dan Widyati, P., 2010. Uji aktivitas senyawa antiplasmodium dari fungi endofit tanaman Artemisia annua L. Majalah Farmasi Indonesia, 21(4), pp. 230–235.

White, T.J., Bruce, T., Lee, S. and Taylor, I., 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. Dalam: Innis, M.A., Gelfand, D.H., Sninsky, J.J. dan White, T.J. eds. PCR Protocols: A Guide to Method and Applications. Academic Press. New York.

DOI: https://doi.org/10.14203/beritabiologi.v17i3.3408

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