Nandang Suharna, Heddy Julistiono
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A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains. 


Monascus sp., Monascus ruber, Monascus purpureus, delimitation, AP PCR fingerprint

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Barnard EL and PF Cannon. 1987. A new species of Monascus from pine tissues in florida. Mycologia 79, 479 – 484.

Cannon PF, SK Abdullah and BA Abbas. 1995. Two new spe-cies of Monascus from Iraq with a key to known spe-cies of the genus. Mycological Research 99, 659 - 662.

Felsenstein J. 1981. Evolutionary Trees from DNA sequences: a maximum likehood approach. Journal of Molecular Evolution 17, 368-376.

Fukatsu T and H Ishikawa. 1994. Differentiation of aphid clones by arbitrarily primed polymerase chain reaction (AP?PCR) DNA fingerprinting. Molecular Ecology 3(3), 187-192.

Gawel NJ. and AC Barlett. 1993. Characterization of differ-ences between whiteflies using RAPD-PCR. Insect Molecular Biology 2(1), 33-38.

Hawksworth DL and JI Pitt. 1983. A New Taxonomy for Monascus Spesies. Australian Journal of Botany 31(115), 1-62.

Karki R, D Pandya, RC Elston, C Ferlini. 2015. Defining “mutation” and “polymorphism” in the era of personal genomics. BMC Med Genomics [Internet]. BMC Med-ical Genomics 8, 37. Available on: diakses tgl. [12/10/2016]

Kubelik AR and LJ Szabo. 1995. High-GC primers are useful in RAPD analysis of fungi. Current Genetics. 28, 284-389.

Menard C, R Brousseau and C Mouton. 1992. Application of polymerase chain reaction with arbitrary primer (AP PCR) to strain identification of Porphyromonas (Bacteroides) gingivalis. FEMS Microbiology Letter 74(2), 163-168.

Stchigel AM, JF Cannon, SK Abdullah, J Guarro. 2004. New and interesting species of Monascus from soil, with a key to the known species. Studies in Mycology 50, 299–306.

Suharna N dan H Julistiono. 1999. Karakter metabolisme etanol pada Monascus sp. MM yang bersifat tahan terhadap etanol konsentrasi tinggi Prosiding Seminar Hasil-Hasil Penelitian Bidang Ilmu Hayat Pusat Antar Universitas Ilmu Hayat IPB. Bogor, 16 September 1999.

Suharna N, Y Kikuchi, and T Fukatsu. 2005. Molecular Phylo-genetic Analysis of Monascus Fungi Based on Internal Transcribed Spacer Region. Biotropia 24, 62 – 68.

Welsh J, RJ Honeycutt, M McCLelland and BWS Sobral. 1990. Parentage determination in maize hibrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theorical Applied Genetic 82: 473-476.

Williams JGK, AR Kubelik, KJ Livak, JA Rafalski and SV Tingey. 1990. DNA polymorphisms amplified by arbitrarily primers are useful as genetic markers. Nu-cleic Acids Research 18(22): 6531-6535

Xu J. 2006. Fundamentals of Fungal Molecular Population Genetic Analyses. Current Issues of Molecular Biology 8, 75–90.


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