KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING

Estu Nugroho, Sabara Putera
10.14203/beritabiologi.v17i1.2869
| Abstract views: 1628 | PDF views: 1022

Abstract

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 – 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton 0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 – 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.

Keywords

Genetic characterization, African Catfish, RAPD, Fingerprinting.

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References

Agbebi, O.T., Ilaboya, D.E. and Adebambo, A.O., 2013. Preliminary characterization of genetic strain in clariid species, Clarias gariepinus and Heterobranchus bidorsalis using microsatellite markers. African Jounal Biotechnology, 12, pp. 364 – 369.

Asuqwo, F.E. and Udoh, J.P., 2002. Patterns of total hydrocarbon, copper and iron in some fish from cross river estuary, Nigeria, West Africa. Journal Applied Ecolology, 3, pp. 91 – 97.

Agriflo. 2013. Lele: Peluang Bisnis dan Kisah Sukses. Penebar Swadaya. Jakarta.

Danis, M., Singh, I.J., Giri, P. and Singh, C.P., 2012. Molecular characterization of two population of catfish Clarias batrachus L using random amplified polymorphic DNA (RAPD) markers. African. Journal of Biotechnolology, 11, pp. 14217 – 14226.

Ferguson, A.J., Taggart, B., Prodohl, P.A., Mc.Meel, O., Thomp-son, C., Stone, C., McGinnity, P. and Hynes, R.A., 1995. The application of molecular markers to the study and conservation of fish population, with special reference to Salmo. Journal of Fish Biology, 47, pp. 103 – 126.

Kottelat, M., 2001. Freshwater of Northern Vietnam. A prelimanary check list of the fishes known on expected to occure in Northern Vietnam with comments on systematics an nomenclature environment and social development unit, east Asia and Pacific region. The World.Bank.Sitesources.worldbank.org./INTBIODIVERSITY/214584-1110958364118/ 20480610/Freshfishes5.pdf (diakses Juli 2016)

Kusmini, I.I., 2009. Karakteristik Fenotipe dan Genotype Hibrida antara Huna Biru (Cherax albertissii) dengan Huna Capit Merah (Cherax quadricarinatus). Skripsi S2. Sekolah Pasca Sarjana, Institut Pertanian Bogor.

Lia, E., 2006. Analisa Keanekaragaman Genetik Ikan Gurame (Osphronemus gouramy Lac) Varietas Bluesafir dengan Menggunakan Metode RAPD. Skripsi S1, Jurusan Pendidikan Biologi. Universitas Pendidikan Indonesia (UPI) Bandung.

Miller, M.P., 1997. Tools for Population Genetics Analysis (TFPGA) version 1.3. Departement of Biological Science. Northern Arizona University, Arizona USA.

Mickett, K., Morton, C., Feng, J., Li, P. and Simmons, M., 2003 Assessing genetic diversity of domestic populations of channel catfish (Ictalurus punctatus) in Alabama using AFLP markers, Aquaculture, pp. 91 – 105.

Nei, M., 1972. Genetic distance between populations. American Nature, 106, pp.283 – 292.

Nakabo, T., 2002. Fishes of Japan with Pictoral Key to the Species). English Edition. Japan Tohoku University Press.

Nugroho, E., 1997. Practical manual on detection of DNA Polymorphism in fish population study. Bulletin of Marine Science and Fisheries, 17, pp. 109 – 129.

Nugroho, E., 2013. Genetic variability of giant gouramu strain revealed by random amplified polymorphism DNA. Indonesian Aquaculture Journal, 8 (1), pp. 107 – 111.

Nugroho, E., Putra, S., Syahdan, M.A., Mayadi, L., Budileksono, S. and Zulkifli, 2015. Efek heterosis dari hibrida ikan lele unggul di Nusa Tengara Barat. Jurnal Riset Akuakultur, 10(1), pp. 33 – 40.

Nugroho, E., Subagja, J., Asih, S. dan Kurniasih, T., 2006. Evaluasi keragaman genetik ikan kancra dengan menggunakan marker mtDNA D-loop dan Random Amplified Polymorphism DNA (RAPD). Jurnal Riset Akuakultur, 1(2), pp. 211 – 217.

Picker, M. and Griffiths, C., 2011. Alien and Invasive Animals, a South African Perspective. Struik Publishers, Cape Town.

Popoola, O.M., Fasakin, E.A. and Awopetu, J.I., 2014. Genetic variability in cultured and wild populations of Clarias gariepinus (Osteichthys: Clariidae) using random amplified polymorphic DNA (RAPD) marker. Croatian Journal of Fisheries, 72, pp. 5 – 11.

Pusdatin. 2015. Kelautan dan Perikanan dalam Angka Tahun 2015. Pusat Data Statistik dan Informasi.Kementerian Kelautan dan Perikanan. Jakarta.

Rashid, J., Tamanna, F.M., Hossain, M.A.R. and Alam, Mds., 2012. Genetic variation in endagered butter catfish, Ompok bimaculatus (Bloch) population revealed by random amplified polymorphic DNA (RAPD) fingerprinting. International Journal of Biosciences, 2(9), pp. 85 – 93.

Skelton, P.H., 2001. A Complete Guide to the Freshwater Fishes of Southern Africa. Struik Publishers, Cape Town.

Theodorakis, C.W. and Bickham, J.W., 2004. Molecular characterization of contaminant indicative RAPD markers. Ecotoxicology, 13, pp. 303 – 309.

DOI: https://doi.org/10.14203/beritabiologi.v17i1.2869

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