Suliasih Suliasih, Sri Widawati
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Nitrogen fixing dan phosphate solubilizing bacteria are important PGPR bacterial in addition of plant nutrients by increasing N and P, especially in saline soils. Experiments were conducted to observe the effects of  soil salinity and bacterial inoculant on growth eggplant (Solanum melongena) at green house of  Microbiology division, Research Center for Biology, The Indonesian Institute of Sciences,  Cibinong. Experiment laid out as factorial based randomized complete design  with three replications. Five levels of watering consisted of (1). Fresh water, (2). NaCl 0.1%, (3). NaCl 1%, (4). Sea water 75 %+25% fresh water, (5). Sea water 100%  as first factors. Five levels of inoculations/biofertilizers consisted of (1).control without fertilizer (K), (2). NPK (P), (3). Nitrogen Fixing bacteria (BPN1), all treatments was repeated 3 times  BPN2, mixed inoculation (BPN1 +  BPN2 + Phosphate Solubilizing Bacteria/BPF) as second factors.  Results showed that biofertilizer application increased growth and yield of eggplant in various levels of salinity at about 21.56%-53.35%  compared to uninoculated plants


Nitrogen fixing bacteria, Phosphate solubilizing bacteria, eggplant

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Amirjani MR. 2001. Effect Of Salinity Str ess On Gr owth, Sugar Content, Pigments And Enzyme Activity Of Rice. International Journal Botany 7, 73-81.

Atlas RM. 2004. Handbook of Microbiological Media, 1390. London. CRC Press.

Bacillio M, H Rodriquez, M Moreno., JP Hernandez, and Y Bashan. 2004. Mitigation of Salt Str ess in Wheat Seedlings by a GFP-Tagged A zospirillum lipoferum. Biology and Fertility of Soils 40,188-193

Berg G, M Alavi, CS Schmidt, C Zachow, D Egamberdieva, F Kamilova and B Lugtenberg. 2013. Biocontr ol and Osmoprotection for Plants under Salinated Conditions. In: Molecular Microbial Ecology of The Rhizosphere. FJ de Bruijn (Ed), 561–573. Wiley-Blackwell, Hoboken.

Bintoro MH 1983. Pengar uh NaCl ter hadap per tumbuhan tanaman terung cv. Senryo dan cv. Akanasu. Buletin Agronomi 14 (3), 32-49.

Bybordi A and E Ebrahimian. 2011. Effect of Salinity Str esson Activity of Enzymes Involved in Nitrogen and Phosphorous Metabolism Case Study : Canola (Brassica napusL.). A sian Journal of A gricultural Research 5 (3), 208-214.

Cherr CM, JMS Scholberg, and R McSorley. 2006. Green Manure Approaches to Crop Production. A gronomy Journal 98, 302-319.

Chinnusamy V, A Jagendorf and JK Zhu. 2005. Under standing and Improving Salt Tolerance in Plants. Crop Science 45, 437–448.

Dobbelaere S, J Vanderleyden, and YY Okon. 2003. Plant Growth-Promoting Effects of Diazotrophs in The Rhizosphere. Critical Review. Plant Science 22, 107-149.

Dodd IC and F Perez-Alfocea. 2012. Micr obial Alleviation of Crop Salinity. Journal of Experimental Botany 63, 3415–3428.

Egamberdieva D and Z Kucharova. 2009. Selection for Root Colonizing Bacteria Stimulating Wheat Growth in Saline Soils. Biology and Fertility of Soils 45 (6), 563-571.

Egamberdieva D. 2011. Sur vival of Pseudomonas Extr emorientalis TSAU20 and P. Chlororaphis TSAU13 In The Rhizosphere of Common Bean (Phaseolus vulgaris) Under Saline Conditions. Plant Soil Environment 57(3), 122–127.

Egamberdieva D and D Jabborova. 2013. Biocontr ol of Cotton Damping-off caused by Rhizoctonia Solani in Salinated Soil With Rhizosphere Bacteria. Asian and Australasian Journal of Plant Science and Biotechnology 7(2), 31–38.

Follet RH, LS Murphy and RL Donahue. 1981. Fertilizer and Soil amandements, 557. Prentice-Hall,Inc, Englewooood Cliffs, New Jersey, USA.

Gamalero E, G Berta, R Bernard and BR Glick. 2009. The Use of Microorganisms to Facilitate the Growth of Plants in Saline Soils. Microbial Strategies for Crop Improvement, 1-22.

Garcia C and T Hernandez. 1996. Influence of Salinity on The Biological and Biochemical Activity of a Calciorthid Soil. Plant Soil 178, 225-263.

Gray EJ and DL Smith. 2005. Intr acellular and Extr acellular PGPR: Commonalities and Distinctions in The Plant-Bacterium Signalling Processes Soil Biology and Bio Chemistry 37, 395-412.

Golpayegani A and HG Tilebeni. 2011. Effect of Biological Fertilizers on Biochemical and Physiological Parameters of Basil (Ociumum basilicm L.) medicine Plant. American Eurasian Journal of Agricultural and Environmental Sciences 11(3), 411- 416.

Hayat R, S. Ali, U Amara, R Khalid and I Ahmed. 2010. Soil Beneficial Bacteria and Their Role in Plant Growth Promotion: A review. A nnals of Microbiology 60, 579-598.

Heidari M and P Jamshid. 2010. Inter action Between Salinity and Potassium on Grain Yield, Carbohydrate Content and Nutrient Uptake in Pearl Millet. Journal of Agricultural and Biological Science 5, 39–46.

Jackson ML. 1973. Soil Chemical Analysis. 1st ed, Vol 1, Prentice Hall, New Delhi, India.

Jamil M, DB Lee, KY Jung, M Ashraf, SC Lee, and ES Rhal. 2006. Effect of Salt (NaCl) Stress on Germination and Early Seedling Growth of Four Vegetables Species. Journal of Central European Agriculture 7, 273–282.

Kaushik A and Sethi. 2005. Salinity Effects on Nitr ifying and Free Diszotrophic Bacteria Populations in The Rhizosphere of Rice. Bulletin of the National Institute of Ecology 15, 139 – 144.

Kloepper JW. 2004. Induced Systemic Resistance and Pr omotion of Plant Growth By Bacillus Species. Phytopathology 94, 1259-1266.

Kurniasih B. 2002. Sifat Per a kar an Beber apa Var ietas Padi Gogo dalam Cekaman Residu Alelopati Gulma. Journal Agrivita 24, 47-52.

Linu MS, J Stephen and MS Jisha, 2009, Phosphate solubilizing Gluconacetobacter sp.,Burkholderia sp. And their potential interaction eith Cowpea (V igna unguiculata (L.) Walp.). International Journal of A griculture Research 4 (2), 79-87.

Lucy M, E Reed, and BR Glick. 2004. Applications of Fr ee Living Plant Growth-Promoting Rhizobacteria. A ntonievan Leeuwenhoek, 86, 1-25.

Lugtenberg B, N Malfanova, F Kamilova and G Berg. 2013. Plant Growth Promotion By Microbes. In: Molecular microbial ecology of the rhizosphere. FJ de Bruijn (Ed), 561–573. Wiley-Blackwell, Hoboken.

Mantri N, V Patade, S Penna, R Ford and E Pang. 2012. Abiotic Stress Responses in Plants: Present And Future. In: Abiotic Stress Responses in Plants: Metabolism, Productivity and Sustainability. Ahmad P and MNV Prasad (eds), 1–19. Springer, New York.

Nelson DR and PM Mele. 2007. Subtle Changes in The Rhizosphere Microbial Community Structure Inresponse to Increased Boron and Sodium Chloride Concentrations. Soil Biology and Biochemistry 39, 340–351.

Ofek M, S Ruppel and Y Waisel. 2006. Effects of Salinity on Rhizosphere Bacterial Communities Associated with Different Root Types of V icia faba L. In: Biosaline Agriculture and Salinity Tolerance in Plants. Ozturk M, Y Waisel, A Khan, and G Gork (eds), 1–21. Birkhauser, Basel.

Ondrasek G, Z Rengel, D Romic, M Poljak, and M Romic. 2009. Accumulation of Non/Essential Elements in Radish Plants Grown in Salt-Affected and Cadmium Contaminated Environment. Cereal Research Communicatios 37, 9–12.

Okon Y, SL Albrecht and RH Burris. 1977. Methodes for Growing Spirillum Lipoferum And for Counting It n Pure Culture And In Association With Plants. Journal of Applied Environmental Microbiology 33, 85 – 88.

Patel D, CK Jha, N Tank and M. Meenu Saraf. 2012. Growth Enhancement of Chickpea in Saline Soils Using Plant Growth-Promoting Rhizobacteria. Journal of Plant Growth Regulator 31(1), 53-62.

Patten CL and BR Glick. 1996. Bacter ial Biosynthesis of Indole-3-Acetic Acid. Canadian Journal Microbiology 42,207-220.

Pliego C, F Kamilova and B Lugtenberg. 2011. Plant Gr owth-Promoting Bacteria: Fundamentals and Exploitation. In:Bacteria In Agrobiology: Crop Ecosystems. Maheshwari DK (Ed), 295–343. Springer, Germany.

Sembiring H dan A Gani. 2005. Adaptasi var ietas padi pada tanah terkena tsunami. (diunduh 26-11-2015).

Subagyono K. 2009. Kerusakan Lahan Pertanian Akibat Tsunami. Balai Penelitian Tanah Bogor. 24 hal.

Subba Rao S. 1994. Mikroorganisme Tanah dan Pertumbuhan Tanaman, 353. Jakarta. Penerbit Universitas Indonesia.

Tabatabai MA and JM Bremner. 1969. ‘Use of p-nitrophenyl phosphate assay of soil phosphatase activity’, Soil Biology and Biolchemistry 1, 301-307.

Tank N and M Saraf. 2010. Salinity-Resistant Plant Growth Promoting Rhizobacteria Ameliorates Sodium Chloride Stress on Tomato Plants. Journal of Plant Interactions 5 (1), 51-58.

Tester M and R Davenport. 2003. Na+ Tolerance And Na+ Transport in Higher Plants. A nnals of Botany 91, 503-27.

Tripathi AK, SC Verma and EZ Ron. 2002. Molecular Characterization of A Salt-Tolerant Bacterial Community in The Rice Rhizosphere. Research in microbiology 153, 579-584.

Yildrim E, AG Taylor and TD Spittler. 2006. Amelior ative Effects of Biological Treatments on Growth of Squash Plant Under Salt Stress. Scientia Horticulturae 111, 1-6.


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