Aryani Leksonowati, Witjaksono Witjaksono, Diah Ratnadewi
| Abstract views: 1086 | PDF views: 1633


Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.


Aquilaria malaccensis, callus induction, cell suspension culture, plant growth regulator

Full Text:



Akter S, MT Islam, M Zulkefeli and SI Khan. 2013. Agarwood Production-A Multidisciplinary Field to be Explored in Bangladesh. Journal of Pharmaceutical and Life Sciences 1(4), 1-11.

Azah MAN, YS Chang, J Mailina, AA Said, A Husni, N Hasnida and N Yasmin. 2008. Comparison of Chemical Profiles of Selected Gaharu Oils from Peninsular Malaysia. The Malaysian Journal of Analytical Sciences 2 (12), 338-340.

Barden A, NA Anak, T Mulliken and M Song. 2000. Heart of The Matter: Agarwood Use and Trade, and CITES Implementation for Aquilaria malaccensis.]. TRAFFIC International. (Diunduh 6 April 2013).

Daud NH. 2013. Optimization of culture parameters for flavonoid production from callus and suspension cultures of the karas tree (Aquilaria malaccensis Lam.). Universiti Putra Malaysia, Selangor [Thesis].

George EF. 1993. Plant Propagation by Tissue Culture Part 1, 574. Exegetic Ltd, England.

Ito M, K Okimoto, T Yagura and Honda G. 2005. Induction of Sesquiterpenoid Production by Methyl Jasmonate in Aquilaria sinensis Cell Suspension Culture. Journal of Essential Oil Research 17, 175-180.

Effects of Plant Growth Regulators, Carbon Sources and pH Values on Callus Induction in Aquilaria malaccensis Leaf Explants and Characteristics of The Resultant Calli. Journal of Forestry Research 25, 535–540.

Jayaraman S and R Mohamed. 2015. Crude Extract of Trichoderma Elicits Agarwood Substances in Cell Suspension Culture of The Tropical Tree, Aquilaria malaccensis Lam. Turkish Journal of Agriculture and Forestry 39, 163-173.

Khairunisa. 2013. Suspension culture of Aquilaria Beccariana Van Teigh. Universiti Malaysia Sarawak, Sarawak. [Thesis].

Murashige T and F Skoog. 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture. Plant Physiology 15, 473–497.

Ng LT, YS Chang and AA Kadir. 1997. A Review on Agar (Gaharu) Producing Aquilaria Species. Journal of Tropical Forest Products 2(2), 272-285.

Okudera Y and M Ito. 2009. Production of Agarwood Fragrant Constituent in Aquilaria Calli and Suspension Cultures. Plant Biotechnology Journal 26, 307-315.

Qi SY, ML He, LD Lin, CH Zhang, LJ Hu and HZ Zhang. 2005. Production of 2-(2-Phenilethyl) Chromones in Cell Suspension Cultures of Aquilaria sinensis. Plant Cell, Tissue, and Organ Culture 83 (2), 217-221.

Saikia M, K Shrivastava and SS Singh. 2012. An Efficient Protocol for Callus Induction in Aquilaria malaccensis Lam. Using Leaf Explants at Varied Concentrations of Sucrose. International Journal of Plant Research 2 (6), 188-194.

Saikia M, K Shrivastava and SS Singh. 2013. Effect of Culture Media and Growth Hormone Callus Induction in Aquilaria malaccensis Lam., A Medicinally and Commercially Important Tree Species of North East India. Asian Journal of Biological Science 6(2), 96-15.

Rijal F. 2011. Pengaruh inokulasi beberapa strain Fusarium spp. terhadap produksi seskuiterpena dari berbagai umur kalus gaharu (Aquilaria malaccensis). Universitas Andalas, Padang. [Skripsi].

Subasinghe SMCUP, DS Hettiarachchi and E Rathnamalala. 2012. Agarwood-Type Resin from Gyrinops walla Gaertn: A New Discovery. Journal of Tropical Forestry and Environment 2(2), 43-48.

Zalikha S. 2013. Cell suspension culture of Aquilaria malaccensis Lamk. Universiti Malaysia Sarawak, Sarawak. [Skripsi].


  • There are currently no refbacks.