KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR)

Dyah Subositi, Harto Widodo
| Abstract views: 777 | PDF views: 809

Abstract

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 

Keywords

Alang-alang (Imperata cylindrica (L.) Beauv.), database, genetic diversity, Inter Simple Sequence Repeats (ISSR)

Full Text:

PDF

References

Imperata cylindrica populatios in Taiwan: mass spectrometry-based proteomic evidence. Journal of Medicinal Plants Research, 2(4), pp. 71–76.

Cheng, K.T. and Chou, C.H., 1997. Ecotypic variation of Imperata cylindrica populations in Taiwan: I.

Morphological and molecular evidences. Botanical Bulletin Academia Sinica, 38, pp. 215–223.

Domayti, F.M., Rania, A.A.Y., Edris, S., Mansour, A., Sabir, G. and Bahieldin, A., 2011. Molecular markers associated with genetic diversity of some medicinal plants in Sinai. Journal of Medicinal Plants Research, 5(10), pp. 200–210.

dos Santos, L.F., de Oliveira, E.J., dos Santos Silva, A., de Carvalho, F.M., Costa, J.L. and Padua, J.G., 2011. ISSR Markers as a tool for the assessment of genetic diversity in passiflora. Biochemical Genetics, 49(7-8), pp. 540–554.

Heikal, A.H., Badawy, O.M. and Hafez, A.M., 2008. Genetic relationships among some stevia (Stevia rebaudiana Bertoni) accesions based on ISSR analysis. Research Journal of Cell and Molecular Biology, 2, pp. 1–5.

Kumar, S.A., Sudisha, J. and Sreenath, H.L., 2008. Genetic relation of coffea and Indian Psilanthus species as revealed through RAPD and ISSR markers. International Journal of Integrative Biology, 3(3), pp. 150–158.

Li, A. and Ge, S., 2001. Genetic variation and clonal diversity of Psammochloa villosa (Poaceae) detected by ISSR markers. Annals of Botany, 87(5), pp. 585–590.

MacDonald, G.E., 2004. Cogongrass (Imperata cylindrica)-biology, ecology, and management. Critical Review in Plant Sciences, 23(5), pp. 367–380.

Matsunaga, K., Ikeda, M., Shibuya, M. and Ohizuni, Y., 1994. Cylindol A, a novel biphenyl ether with 5-lipoxygenase inhibitory activity, and a related compound from Imperata cylindrica. Journal of Natural Products, 57(9), pp. 1290–1293.

Matsunaga, M., Shibuya, M. and Ohizuni, Y., 1994. Cylindrene, a novel sesquiterpenoid from Imperata clylindrica with inhibitory activity on contractions of vascular smooth muscle. Journal of Natural Products, 57(8), pp. 1183–1184.

Matsunaga, M., Shibuya, M. and Ohizuni, Y., 1994. Graminone B, a novel lignan with vasodilative activity from Imperata cylindrica. Journal of Natural Products, 57(12), pp. 1734–1736.

Matsunaga, M., Shibuya, M. and Ohizuni, Y., 1995. Imperanene, a novel phenolic compound with platelet aggregation inhibitory activity from Imperata cylindrica. Journal of Natural Products, 58(1), pp. 138–139.

Moustafa, M.F., Hesham, A.E-L., Quraishi, M.S. and Alrumman, S.A., 2016. Variations in genetic and chemical constituents of Ziziphus spina-christi L. populations grown at various altitudinal zonation up to 2227 m height. Journal of Genetic Engineering and Biotechnology, 14(2), pp. 349–362.

Nei, M. and Li, W.H., 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Science, 76(10), pp. 5269–5273.

Parkavi, V., Vignesh, M., Selvakumar, K., Mohamed, J.M., and Ruby, J.J., 2014. Antibacterial activity of aerial parts of Imperata cylindrica (L) Beauv. International Journal of Pharmaceutical Sciences and Drug Research, 4(3), pp. 209–212.

Pudjiharta, A., Widyati, E., Adalina, Y. dan Syarifuddin, H.K., 2008. Kajian teknik rehabilitasi lahan alang-alang. Info Hutan, 5(3), pp. 219-230.

Reddy, M.P., Sarla, N. and Siddiq, E.A., 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128 (1), pp. 9–17.

Rohlf, F.J., 1998. NTSYS-Pc. Numerical taxonomy and multivariate analysis system version 2.0. Exeter Software. New York.

Rout, G.R., 2006. Identification of Tinospora cordifolia (Willd.) Miers ex Hook.f &Thomas using RAPD markers Journal of Biosciences, 61(1-2), pp. 118–122.

Sellers, B.A., Ferrell, J.A., MacDonald, G.E., Langeland, K.A. and Flory, S.L., 2012. Cogongrass (Imperata cylindrica) Biology, Ecology, and Management in Florida Grazing Lands. Agronomy Department, UF/IFAS Extension.

Subramaniam, S., and Sivasubramaniam, A., 2015. Tradition to therapeutics: sacrificial medicinal grasses Desmostachya bipinnata and Imperata cylindrica of India. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 14(3), pp. 156 – 170

Touil, L., Bao, A.K., Wang, S.M., and Ferchichi, A., 2016. Genetic diversity of Tunisian and Chinese Alfalfa (Medicago sativa L.) revealed by RAPD and ISSR markers. American Journal of Plant Sciences, 7(6), pp. 967–979.

Wu, Y.B., Zheng, L.J., Yi, J., Wu, J.G., Chen, T.Q., and Wu, J.Z., 2013. Quantitative and chemical fingerprint analysis for the quality evaluation of Receptaculum nelumbinis by RP-HPLC coupled with hierarchical clustering analysis. International Journal of Molecular Science, 14(1), pp. 1999–2010.

Xuan, T.D., Toyama, T., Fukuta, M., Khanh, T.D., and Tawat, S., 2009. Chemical interaction in the invasiveness of cogongrass (Imperata cylindrica (L.) Beauv.). Journal of Agricultural and Food Chemistry, 57(20), pp. 9448–9453.

Yulita, K.S., Bora, C.Y., Arsa, I.G.B.A., dan Murniningsih, T., 2015. Analisis fenetik jagung ras lokal Nusa Tenggara Timur umur genjah berdasarkan karakter agronomi dan inter short sequence repeats. Berita Biologi, 14(3), pp. 277–286.

Zhou, Y., Zhou, C., Yao, H., Liu, Y., and Tu, R., 2008. Application of ISSR markers in detection of genetic variation among Chinese yam (Dioscorea opposita Thunb) cultivars. Life Science Journal, 5(4), pp. 6–12.

Ziekiewicz, E., Rafalski, A., and Labuda, D., 1994. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genome, 20(2), pp.176–183.


Refbacks

  • There are currently no refbacks.