MIKROBA ENDOFIT DARI TANAMAN SRIKAYA (Annona squamosa L.) SEBAGAI PENGHASIL ANTIMIKROBA Staphylococcus aureus DAN Candida albicans

Ruth Melliawati, Sunifah Sunifah
| Abstract views: 609 | PDF views: 615

Abstract

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant  (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.

Keywords

Endophytic microbes, sugar-apple (Annona squamosa), antimicrobial activity, Staphylococcus aureus, Candida albicans.

Full Text:

PDF

References

Cacabuono AC and AB Pomilio. 1997. Alkaloids from endophyte-infected Festua argentina. Journal of Ethnopharmacology 57, 1-9.

Carrol GC. 1988. Fungal Endophytes In Stem and Leaves from Latent Atgogens to Mutualistic Symbiont. Ecology 69, 2–9.

Clay K. 1988. Fungal Endophytes of Grasses: A Defensive Mutualism Between Plant and Fungi. Ecology 69(1), 10–16.

Fabry W, PO Okemo and R Ansorg. 1998. Antibacterial activity of East African medicinal plants. Journal of Ethnopharmacology 60, 79-84.

Fathoni A, M Ilyas, Praptiwi, AH Cahyana dan A Agusta. 2013. Skrining dan isolasi metabolit aktif antibakteri kultur jamur endofit dari tumbuhan Albertisia papuana Becc. Berita Biologi 12(3), 307-323.

Ferlin W, K Ruslan dan K Siti. 2007. Telaah Fitokimia Daun Srikaya (Annona squamosa L.) yang berasal dari Dua Lokasi Tumbuh. Fakultas Farmasi ITB, Bandung.[Skripsi]

Gritter RJ, M Bobbitt and AE Schwarting. 1991. Pengantar Kromatografi, 266 hal. Penerbit ITB, Bandung

Madigan MT, JM Martinko, PV Dunlap, DP Clark. 2008. Biology of Microorganism,1032 hal 12th Ed. Pearson, San Fransisco.

Melliawati R, DN Widyaningrum, AC Djohan dan H Sukiman. 2006. Pengkajian Bakteri Endofit Penghasil Senyawa Bioaktif untuk Proteksi Tanaman. Biodiversitas 7(3), 221-224.

Melliawati R, E Ismawati, F Octavina. 2007. Kapang Endofitik Potensial Sebagai Penghasil Anti Mikroba Patogen. Jurnal Berkala Ilmiah Biologi 6(1), 9-17.

Melliawati R dan PS Wulandari. 2008. Kapang Endofit dari Taman Nasional Gunung Halimun sebagai Pelczar MJ Dan ECS Chan. 2006. Dasar-dasar Mikrobiologi, 481-593 Edisi ke-2. UI Press, Jakarta.

Rizzo I, E Varsavky, M Haiduhososki and H Frade. 1997. Macrocyclic trichothecence in Barcharis coridifolia plants and endophytes and Baccharis artemisioides plants. Toxicon 35, 753-757.

Santoso S, S Supardiyah dan M Yulfira. 2003. Obat Tradisional untuk Penyembuhan Penyakit DM dari Pengobatan Tradisional (BATRA) di DKI Jakarta, Yogyakarta dan Surabaya. Jurnal Ekologi Kesehatan 2(2), 239-248.

Strobel G And B Daisy. 2003. Bioprospecting for Microbial Endophyte and Their Natural Product. Mikrobiology and Molecular Biology Review. 67(4), 491–502.

Sukara E, R Melliawati and S Saono. 1992. Amylases Production From Cassava by An Indigenous Yeast. Journal Science Technology Development 9(1), 157-168.

Strobel GA, WM Hess, EJ Ford, RS Sidhu and X Yang. 1996. Taxol from fungal endophytes and the issue of biodiversity. Journal of Industry Microbiology 17, 417-423.

Tan RX and WX Zou. 2001. Endophytes : A Rich source of functional metabolites. Natural product reports. 18 (4), 448-459.

Tomita F. 2003. Endophytes in Southeast Asia and Japan: their taxonomic diversity and potential application. Fungal Diversity. 14, 187-204.

Volk dan Wheeler. 1990. Mikrobiologi Dasar, 396 Jilid 2 Edisi ke-5. Diterjemahkan oleh Sumarto Adi Sumartono. Erlangga, Jakarta.


Refbacks

  • There are currently no refbacks.