Diyah Martanti, Yuyu Suryasari Poerba, Herlina Herlina
| Abstract views: 491 | PDF views: 550


Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular characteristic of diploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primers have been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed that the hybrid combination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83%, 51.25% and 47.21% respectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identification of male parents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22%), 6 (7.5%) and 11 (12.64%) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highest polymorphic bands was obtained by SNMT (83.9%) and the lowest obtained was performed by MDMT (71.25%). From this result, RAPD markers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas.


Banana breeding, hybrid diploid bananas, RAPD marker

Full Text:



Darnell, J.E., Baltimore, D. and Lodish, H.F., 1990. Molecular Cell Biology. Scientific American Books, Inc.

Delaporta, S.L., Wood, J. and Hiks, J.B., 1983. A plant DNA minipreparation, Version II. Plant molecular biology reporter, 1(4), pp. 19-21.

Ellstrand, N.C., 2003. Current knowledge of gene flow in plants: implication of transgene flow. Philosopical transactions of the royal society of London, series B. Biological Sciences, 358, pp.1163-1170.

Hakkinen, M. and Wallace, R., 2011. Genetic Resources for Banana Improvement. in Banana Breeding: Progress and Challenges. Ed. Pillay M and A Tenkouano. CRC Press, Florida.

Huang, S.C., Tsai, C.C. and Sheu, C.S., 2000. Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers. Botanical Bulletin of Academia Sinica, (41), pp. 257-262.

Huchett, B.I. and Botha, F.C., 1995. Stability and potential use of RAPD markers in a sugarcane genealogy. Euphytica, (86), pp.117-125.

Inthawong, S., Bundithya, W., Kuanprasert, N. and Apavatjrut, P., 2006. Analysis of Intersectional hybrids of Dendrobium by RAPD technique. Kasetsart Journal (Natural science), 40, pp. 456-461.

Kayat, F., Javed, M.A., Ho, Y.W. and Othman, R.Y., 2004. Identification of molecular markers for disease resistance genes to Fusarium oxysporum f. sp. cubense in Musa acuminata ssp. malaccensis for marker assisted selection (MAS). The 4th Annual Seminar of National Science Fellowship. pp. 40-44.

Nasution, R.E., 1991. A Taxonomic study of the Musa acuminata Colla with its intraspecific taxa in Indonesia. Dissertasion. Memoirs of the Tokyo University of Agriculture, 32, pp. 121.

Sanchez, G.M., Nava, M.T.B., Gonzales, S.G., Santoz, M.O., Youssef, M. and Medrano, R.M.E.G., 2015. Genetic Diversity in Bananas and Plantains (Musa spp.). Intechopen. pp. 95-98. (Accessed 20 January 2016) DOI: 10.5772/59421.

Sikora, B. and Nowaczyk, P., 2014. Application of RAPD technique for identification of interspesific hybrids from genus Capsicum. Acta scientiarum polonorum hortorum cultus,13(10), pp. 155-156.

Silva, S.O., Souza, J.R.M.T., Alves, E.J., Silveira, J.R.S. and Lima, M. 2001. Banana breeding program at Embrapa. Crop Breeding and Applied Biotechnology, 1, pp. 399-436.

Simmonds, N.W., 1962. The Evolutions of Bananas. Longmans, Green and Co. Ltd. London.

Tenkouano, A., Crouch, J.H., Crouch, H.K. and Ortiz, R. 1998. Genetic diversity, hybrid performance and combining ability for yield in Musa germplasm. Euphytica, 102, pp. 281-288.

Tenkouano, A., Vuylsteke, D., Okoro, J., Makumbi, D., Swennen, R. and Ortiz, R. 2003. Diploid banana hybrids TMB2x5105-1 and TMB2x9128-3 with good combining ability, resistance to Black Sigatoka and nematodes. HortScience, 38 (3), pp. 468-472.

Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V., 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic acid research, 18 , pp. 6531-6535.

Wolff, K., Ziettiewicz, E. and Hofstra, H., 1995. Identification of Chrysanthemum cultivars and stability of DNA fingerprint patterns. Theoretica and applied genetics, 91, pp. 439-447.


  • There are currently no refbacks.