Nunik Sulistinah, Bambang Sunarko
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Succinic acid (HOOC(CH2)2COOH) is commercial compound that primarily used in food products. This compound also used in textile industry for colouring process, drugs industry, paint, pernist and photography slice. Succinic acid usually produced in industry by chemical reaction and produce cyanide acid as by product. The objective of this research was to study the utilization of Pseudomonas sp. in produce succinic acid and to characterize of involved enzymes. The results showed that the enzyme optimally produce in log phase. The production rate of succinic acid formation was 0,982 mM/ml.h with the decrease of substrate consumption was 1,235 mM/ml.h. The yield of succinic acid during 81 hours fermentation was 81%. The optimum pH, temperature, Km and Vran were 7, 27°C, 90 mM, and 0,0002 mM/ml.h.


biotransformasi, kinetika, suksinonitril, Pseudomonas sp

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Acharya A and AJ Desai. 1997. Studies on utilization of acetonitrile by Rhodococcus erythropolis A10. Journal Microbiology & Biotechnology 13, 175-178

Banerjee A, R Sharma and UC Banerjee. 2002. The nitrile-degrading enzymes: current status and future prospects. Appl. Microbiology Biotechnology 60, 33-44.

Drauz K and H Waldmann. 1995. Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, 365-367. VCH, Weinheim.

Fitri EY. 2005. Kinetika Fermentasi dan Karakteristik Enzim yang Terlibat Dalam Proses Produksi Asam Adipat oleh Pseudomonas sp. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam-IPB.

Gerhardt, P, RGE Murray, WA Wood and NR Krieg. 1994. Methods for General and Molecular Bacteriology. ASM, Washington D.C. Hodgson E and EL Patdricia. 1987. A Text Book of Modern Toxicology. Elsevier, New York.

Mart'ynkov'a L and V Mylerov'a. 2003. Synthetic applications of nitrile-converting enzymes. Curr. Org. Chem. 7, 1279-1295.

Meyer O and HG Schlegel. 1983. Biology of aerobic carbon monoxide oxidizing bacteria. Ann. Rev. Microbiol. 37, 277-310.

Nagasawa T, H Nanaba, K Ryuno, K Takeuchi and H Yamada. 1987. Nitrile hydratase of Pseudomonas chlororaphis B23. Eur. J. Biochem. 162, 691-698.

Pfennig, N. 1974. Rhodopseudomonas globiformis sp., a new species of the Rhodospirillaceae. Arch. Microbiol 100, 197-206.

Sunarko B and N Sulistinah. 2008. Karakterisasi enzim nitril hidratase dan amidase dari Pseudomonas sp. BP3 dalam biokonversi adiponitril menjadi asam adipat. Jurnal Biologi Indonesia V(2), 173-186.

Thontowi A, EW Pamuji and B Sunarko. 2004. Biotransformasi Adiponitril oleh Bacillus licheniformis BA2. Berk. Penel. Hayati 10, 25-30.

Wu ZL and ZY Li. 2002. Enhancement of enzyme activity and enantioselectivity via. Cultivation in nitrile metabolism by Rhodococcus sp. CGMCC 0497. Biotechnol. Appl. Biochem 35, 61-67

Wang M-X, J Liu, D-X Wang and Q-Y Zheng. 2005. Synthesis of optically active a-methylamino acids and amides through biocatalytic kinetic resolution of amides. Tetrahedron: Asymmetry 16, 2409-2416.


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