ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI MINYAK ATSIRI DAUN KEMBANG BULAN {Tithonia diversifolia (Hemsley) A. Gray}
Hartati Soetjipto, Lusiawati Dewi, Sentot Adi Prayitno
| Abstract views: 2816
| 
PDF views: 5373
Abstract
Isolation and identification of chemical compounds and antibacterial compounds of the essential oil of Kembang bulan or Japanese sunflower {Tithonia diversifolia (Hemsley) A. Gray} leaves showed that the essential oil contain 29 components. The main components were caryophyllene (27.76%), nerolidol (21.81%),caryophyllene oxide (7.06%), copaene (6.41%) and bicylogermacrene (4.90%). The oil was screened for their antibacterial activity through bioautography method. Two antibacterial spots which had Rf 0.49 and Rf 0.61were obtained. The spot with Rf 0.49 was a mixture of 21 compounds with main compounds were heneicosane, nonacosane and tetratetracontane, while the spot with Rf 0.61 consisted of 22 compounds, which the main compound was nerolidol.
Keywords
Full Text:
PDFReferences
Arruda DC, FL D'Alexandri, AM Katzin and SRB Uliana. 2005. Antileishmanial Activity of the Terpcne Nerolidol. Antimicrobial Agents and Chemotherapy 49(5), 1679-1687.
Burt S. 2004. Essential oils : their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology 94, 223-253.
Dzulkarnain B dan DCA Sundari. 1996. Tanaman Obat Bersifat Antibakteri di Indonesia. Dalam: A Ajizah. 2004. Sensitivitas Salmonella typimurium terhadap ekstrak daun Psidium guajava L. Bioscientiae 1(1), 31-38.
Erindyah RW dan Maryati. 2002. Uji aktivitas antibakteri minyak atsiri pinus (Pinus merkusii Jung de Vr.) terhadap Staphylococcus aureus dan Escherichia coli.Jurnal Ppenelitian Sains & Teknologi 3(2), 130-139.
Guenther E. 1987. Minyak Atsiri. Jilid 1, Universitas Indonesia, Jakarta. Hamburger MO and GA Cordell. 1987. A direct bioautographic TLC assay for compounds possesing Antibacterial Activity. Journal of Natural Products 50, 19-22.
Lee Sook-Jin, Han Je-Ik, Lee Geun-Shik, Park Mi-Jin, Choi Ing-Gyu, Na Ki-Jeong and Jeung Eui-Bae. 2007. Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model (medicinal chemistry). Biological and Pharmaceutical Bulletin 30(1), 184-188.
Lestari R, H Soetjipto and AI Kristijanto. 2003. Larvicidal effect of Japanese sunflower (Tithonia diversifolia (Hemsley) A. Gray) extract against mosquitoes larvae Aedes aegypti Linnaeus. Asian Symposium on Medicinal Plants, Spices and Other Natural Products XI. Kunming, China, October 26- 30,2003.
Moronkola DO, IA Ogunwande, TM Walker, Setzer WN and Oyewole IO. 2007. Identification of the main volatile compounds in the leaf and flower of Tithonia diversifolia (Hemsl.) Gray. Journal of Natural Medicines 61(1), 63-66. Springer, Japan.
Pramono E. 2002. Prospek dan potensi pengembangan komoditas agromedicine di Indonesia. Prosiding Simposium Nasional II Tumbuhan Obat dan Aromatik APINMAP, Bogor 8-10 Agustus 2001(I), 31 - 37. BP Naiola et al. (Editor). Pusat Penelitian Biologi-LIPI, Bogor.
Ristanti FW, H Soetjipto dan KH Timotius. 2006. Identifikasi citral dan aktivitas antibakteri minyak atsiri daun kemangi (Ocimum basilicum Linn.). Prosiding Seminar Nasional Kimia dan Pendidikan Kimia, Semarang 11 November 2006,239 -246. WST Astuti et al. (Editor). Jurusan Kimia, Fakultas MIPA-Universitas Negeri Semarang.
Shahecn EA, Ikawa JY and Blum RL. 1998. US Patent 6361787, Enhanced antimicrobial composition, March 26, 2002.
Susilowati IT. 2004. Pengujian aktivitas antibakteri ekstrak kasar daun, bunga dan akar tumbuhan kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] dalam berbagai fraksi pelarut. Fakultas Sains dan Matematika, Universitas Kristen Satya Wacana, Salatiga.(belumdi dipublikasi).
Yuharmen, Y Eryanti dan Nurbalatif 2002. Uji aktivitas antimikroba minyak atsiri dan ekstrak metanol lengkuas (Alpinia galanga). Jurnal Natur Indonesia 4(2).
http://www.unri.ac.id/iurnal/iurnal natur/vol4(2)/vuharmen.pdf
Refbacks
- There are currently no refbacks.