PENGARUH EKSTRAK AIR DAN ETANOL Alpinia spp. TERHADAP AKTIVITAS DAN KAPASITAS FAGOSITOSIS SEL MAKROFAG YANG DIINDUKSI BAKTERI STAPHYLOCOCCUS EPIDERMIDIS SECARA IN-VITRO'

Dewi Wulansari, Praptiwidan Chairul
10.14203/beritabiologi.v9i4.2006
| Abstract views: 473 | PDF views: 649

Abstract

Ellianol 70% and water exlracls of Alpinia spp. i.e. Alpinia zemmbei. A. kaisumadai. A. malaccensis and A. officinarum were examined for their impact in in-vitro phagocytosis activity and capacity of mouse (Mus musculus) peritoneum macrophage induced by Staphylococcus epidermidis.The extract concentrations used in this experiment were 0: O.I: 1.0: 10: 100: and 1000 µg/ml,Imboost (Echinacea pwpurea extract) 1000 µg/ml was used as positive control while distilled water as negative control.The assay results showed that all of the extracts were active to promote phagocytosis activity and capacity of macrophage cells.The phagocytosis acitivity and capacity were increased by increasing extract concentration, and ethanol extract showed better activity than water extract. Alpinia officinarum and A. kaisumadai extracts reveal better phagocytosis activity and capacity than others. Activity and capacity of phagocytosis of each concentration was significantly (P<0.05) different each other as well as with negative control. There is significant difference among each extracts and positive control at 1000  µg/ml

Keywords

Zingiberaceae. Alpinia. inuinomodulalor. fagositosis.

Full Text:

PDF

References

Agarwal SS and VK Singh. 1999. Immunomodulators: A Review of Studies on Indian Medicinal Plants and Synthetic Peptides, Part I: Medicinal Plants. PINSA B65(3&4), 179-204.

Anonim. 1995. Farmakope Indonesia Edisi-1Y. Departemen Kesehatan Republik Indonesia, Jakarta. Anonim. 2004. Kusta di Indonesia Peringkat Keempat di Dunia. Suara Pembaharuan 14 Januari 2004, 11. Jakarta.

An N, Z Zou, Z Tian, X Luo, S Yang and L Xu. 2008. Diarylheptanoids from the rhizomes of Alpinia officinarum and their anticancer activity, Fitoterapia 79(1), 27-31.

De la Fuente M. 2002. Effects of antioxidaats on immune system ageing. European Journal of Clinical Nutrition. 56(Suppl.3), S5-S8.

D'lncalci M, WP Steward and AJ Gescher. 2005. Use of cancer chemopreventive phytochemicals as antineoplastic agents. Lanset Oncol. 6, 899 - 904.

Ebadi M. 2002. Pharmocodynamic Basis of Herbal Medicine. CRC Press, New York.

Ichinose M, M Sawada, K Sasaki and Y Oomura. 1998. Enhancement of phagocytosis in mouse peritoneal macrophages by fragment of acidic fibroblast growth factor (aFGF). Inter. J. Immunopharm. 20, 193-204.

Kuntorini EM. 2005. Botani Ekonomi Suku Zingiberaceae sebagai Obat Tradisional oleh Masyarakat di Kotamadya Banjarbaru. Bioscientiae 2(1), 25-36.

Lee SE, HT Shin, HJ Hwang and JH Kim. 2003. Antioxidant activity of extracts from Alpinia katsumadai seed. Phytother Res. 17(9), 1041-1047.

Lin LY and Chiung. 2008. Alpinia zerumbet potentially elevates High-Density Lipoprotein cholesterol level in hamsters. J. Agric. Food Chem. 56(12), 4435-4443.

Ly TN, M Shimoyamada, K Kato and Yamauchi R. 2004. Antioxidative compounds isolated from the rhizomes of smaller galanga (Alpinia officinarum Hance). Bio Factors 21, 305-308.

Mills S and K Bone. 2000. Principles and Practice of Phytotherapy, Modern Herbal Medicine. Churchill Livingstone Edition, New York.

Subramanian K, C Selvakkumar, S Mecnakshisundaram, A Balakrishnan and B Subhadra Lakshmi. 2008. Extract of Alpinia officinarum suppresses enteropathogenic Eschericia coli (EPEC) lipopolysaccharide (LPS) induced inflammation in J774 A.I macrophages. Journal of Health Science 54(1), 112-117.

Wagner H and K Jurcic. 1991. Assay for immunomodulation and effect on mediators of inflammmation, In: Methods in Plants Biochemistry: Assay for Bioactivity VI. PM Dey and JB Harborne (Eds.). Academic Press, London.

Yadav PN, Z Liu and MM Rafi. 2003. A diarylheptaoid from lesser galangal (Alpinia officinarum) Inhibits proinflanraiatory mediators via inhibition of mitogenactivated protein kinase, p44/42, and transcription factor nuclear factor-B. Journal of Pharmacology and Experimental Therapeutics. 305, 925-931.

DOI: https://doi.org/10.14203/beritabiologi.v9i4.2006

Refbacks

  • There are currently no refbacks.