Nandang Suharna
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Study was carried out to know intraspecific variation within Monascus purpureus in Chinese red rice (angkak) collected from a number of cities in East Java, covering isolation, characterization and identification of Monascus based on cultural and microscopic characters.Results showed that cultural and microscopic observation on 19 representative isolates of Monascus which each isolate was observed from 19 sample of angkak showed variations among those isolates.Those characters were rate of growth, pigmentation,crystalline hyphae, spore size and cleistothecium diameter.These results indicated that there are intraspecies variaty on M.purpureus used in angkak production. Moreover, cultural and morpholological characters such as colony diameter and askomata seemingly can not be used as taxonomic characters.


Monascus, keragaman intraspesies, angkak, morfologi, karakterisasi.

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Barnard EL and PF Cannon. 1987. A new species of Monascusfrom pine tissues in Florida. Mycologia 79, 479-484.

Benny GL and JW Kimbrough. 1980. A synopsis of the orders and families of Plectomycetes with keys to genera. Mycotaxon 12, 1-91.

Cabrera AL. 1994. Enciclopedia Argentina de Agricultura y Jardineria. Tomo II. Regiones Fitogeogrdficas Argentinas. Editorial Acme SACI, Argentina.

Cannon PF, SK Abdullah and BA Abbas. 1995. Two new species of Monascus from Iraq, with a key to known species of the genus. Mycological Research 99, 659-662.

Domsch KH, W Gams and TH Anderson. 1980. Compendium of Soil Fungi. Academic Press, London.

Erdogrul O and Azirak S 2004. Review of the studies on the red yeast rice (Monascus purpureus). Turkish Electronic Journal of Biotechnology Vol.2: 37-49

Eriksson OE and DL Hawksworth. 1991. Outline of ascomycetes - 1990. Systema Ascomycetum 9, 39-141. Hawksworth DL, BC Sutton and GC Ainsworth (eds.). 1983. Ainsworth and Bisby's Dictionary of the Fungi. 7th ed. Commonwealth Mycological Institute, Kew, Surrey, UK.

Hawksworth DL, PM Kirk, BC Sutton and DN Pegler (eds.). 1995. Ainsworth and Bisby's Dictionary of the Fungi. 8* ed. International Mycological Institute, Kew, Surrey, UK.

Hawksworth DL and JI Pitt. 1983. A new taxonomy for Monascus species based on cultural and microscopical characters. Australian Journal of Botany 31, 51-61.

Hesseltine CW. 1965. A millennium of fungi and fermentation. Mycologia 57, 149_197.

Hocking AD and JI Pitt. 1988. Two new species of xerophilic fungi and a further record of Eurotium halophilicum. Mycologia 80, 8288.

Jiyuan Ma, LI Yongguo, Li Qing Ye, Jing Li, Yanjun Hua, Dajun Ju, Decheng Zhang, Raymond Cooper and Michael Chang. 2000. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agric. Food. Chem. 48, 5220-5225.

Kimbrough JW. 1981. Cytology, ultrastructure, and taxonomy of Thelebolus (Ascomycetes). Mycologia 73, 1_27.

Kornerup A and JH Wanscher. 1984. Methuen Handbook of Colour. 3rd ed. Erye Methuen, UK.

Kumar S, K Tamura and M Nei. 1993. MEGA: Molecular evolutionary genetic analysis v. 1.0. The Pennsylvania State University Park, USA.

Li Z. 1982. A new species of the genus Monascus. Ada Microbiologica Sinica 22, 118-122.

Lin CF. 1975. Studies on the Monascus isolated from the starter of kaoliang brandy. Chinese Journal of Microbiology 8,152-160.

Malloch D. 1981. The plectomycetes centrum. In: Ascomycete Systematics, 73-91. DR Reynolds (ed.). Springer, USA.

Park HG and S-C Jong. 2003. Molecular characterization of Monascus strains based on the D1/D2 regions of LSU rRNA genes. Mycoscience 44, 25-32.

Pattanagul P, R Pinthong, A Phianmongkhol and N Leksawasdi. 2007. Review of angkak production (Monascus purpureus) Chiang Mai J. Sci. 34(3), 319- 328.

Pitt JI and AD Hocking. 1985. Fungi and Food Spoilage. Academic Press, Australia.

Saitou N and M Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406-425.

Stchigel AM, JF Cano, SK Abdullah and J Guarro. 2004. New and interesting species of Monascus from soil, with a key to the known species. Studies in Mycology 50, 299-306.

Steinkraus KH, RE Cullen, CS Pederson, LF Nellis and BK Gavitt 1983. Handbook of Indigenous Fermented Foods, 547 553. Marcel Dekker, Inc. New York.

Wong HC and YS Bau. 1977. Pigmentation and antibacterial activity of fast-neutron and x-ray-induced strains of Monascus purpureus Went. Plant Physiology 60, 578-581.

Wong HC, YC Lin and PE Koehler. 1981. Regulation of growth and pigmentation of Monascus purpureus by carbon and nitrogen concentrations. Mycologia


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