PENGGUNAAN VAKSIN Aeromonas hydrophila: PENGARUHNYATERHADAP SIANTAN DAN IMUNITAS LARVA IKAN PATIN (Pangasionodon hypophthalmus)

Angela Mariana Lusiastuti, Wartono Hadie
| Abstract views: 592 | PDF views: 597

Abstract

Aeromonas hydrophila is a pathogen that often causes considerable losses in the area of freshwater fish fanning. Vaccination is one way to simulate parent catfish make specific immunity. Specific immunity generated by the parent will be forwarded through the oocytes produced during a certain time span. The aim of this research was to know the effect and the effectivity of using hydrovac vaccine with and without the complete adjuvant. This research was done on Patin fish Pangasionodon hypophthalmus whose givng Hydrovac 0.4 ml/kg of body weight. The comparation between complete adjuvant and vaccine was 1:1. Injection was done by intra peritoneal for three mothers each with and without complete adjuvant. Injection was done at gonad maturity level II. The result showed that antibody were positively detected on mother serum which used adjuvant or not. On larva stage, antibody was detected until four weeks old. While on 2 weeks old of larva, the concentration of titer antibody was very high and raised the dilution of 1: 2048. Survival rate of juvenile which their mother got a vaccine raised 93%, was better than 73%-63% using mother without vaccine. Booster immersion of hydrovac vaccine could give preferably at the end of three weeks old or in the beginning of fourth weeks old of larva.

Keywords

Vaksin hydrovac, adjuvan, ikan pati, Pangasius hypophthalmus, sintasan larva, imunitas larva

Full Text:

PDF

References

Anderson DP and AK Siwicki. 1993. Basic Hematology and Serology for Fish Health Programs. Second Symposium on Diseases in Asian Aquaculture. Phuket Thailand.

Anderson DP. 1974. Diseases of Fishes. In: SF Sniszko and HR Axelrod (Ed). Book 4: Fish immunology. Neprure City.

Anonimous. 2006. Produksi Perikanan di Jawa Barat. http://www.osha.gov (akses 10 Mei 2007) Anonimous. 2007. Budidaya Ikan Patin. http://rivafauziah.wordpress.com/2007/06/03/budidaya-ikanpatin/(akses 8 Januari 2010)

Anonimous. 2008. Vaccine for Tilapia in aquaculture. http://www.aquaticcommunity.com/tilapia/vaccin, es.php

Baratawidjaya KG 2006. Imunologi Dasar, 27-55. Fakultas Kedokteran Universitas Indonesia. Penerbit Gaya Baru Jakarta.

Ellis AE.1988. General Principles of Fish Vaccination, 1-19. Academic Press, London.

Kabata Z. 1985. Parasiter and Disease of Fish Cultured in Tropic. Taylor and Franchis Ltd. London.

Kawahara E, T Inarimori, Aurano, S Nomura and Y Takahashi. 1993. Transfer of maternal immunity of white spotted char, Salvalinus leucomaenis against furunculosis. Nippon Suisan Gakkaishi 59(3), 567.

Manning MJ, MF Grace, CJ Secombes. 1982. Ontogenic aspects of tolerance and immunity in carp and rainbow trout: Studies on the role of the thymus. In: Immunology and Immunization of Fish. Development and Comparative Immunology, Suppl. 2, 75-22.

Mor A and RR Avtalion. 1990. Transfer of antibody activity from immunized mother to embryo in Tilapia. J. Fish Biol. 37, 249-255.

Tatner MF and MJ Manning. 1985. The ontogenic development of the reticulial system in the rainbow trout, Salmo gairdneri. J. Fish Dis. 8, 35-41.

Taukhid dan D Bastiawan. 1995. Pengaruh Vaksinasi Maternal Anti-Aeromonas hydrophila Terhadap Benih Ikan Lele Dumbo (Clarias sp.) yang Dihasilkannya. Balitkanwar.

Thome MT, RJ Roberts, MTatner and P Ward. 2006. The effects of the use of potassium alum adjuvants in vaccines against vibriosis in rainbow trout, Salmo gairdneri. Journal of Fish Diseases 7(issue 2), 91-99.

Zairin MJ. 2000. Annual changes in ovarian Mmaturity of female thai catfish (Pangasius hypophthalmus) reared in a cultured pond. Biotropia 15, 48-57.


Refbacks

  • There are currently no refbacks.