PENINGKATAN KUALITAS NUTRISI TEPUNG DAUN LAMTORO SEBAGAI PAKAN IKAN DENGAN PENAMBAHAN EKSTRAK ENZIM CAIRAN RUMEN DOMBA

Indira Fitriliyani, Enang Harris, Ing Mokoginta, Nahrowi Nahrowi
10.14203/beritabiologi.v10i2.1965
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Abstract

The aim of this experiment is to evaluate the nutrient quality of leucaena leaf meal (LLM) with addition of sheep rumen fluid enzyme for nile tilapia feed and incubated in vitro either for 2 or 24 hours. This experiment was arranged in a completely randomized design with 6 treatments and 3 replications. Those treatments were enzyme levels of 0, 20, 40, 60, 80, and 100 ml/ kg LLM. Results showed that nutrient quality of LLM with addition of sheep rumen fluid enzyme that incubated for 24 hours had the best result compared to that incubated for 2 hours. This finding significantly affected (PO.05) on the increase of sugar release (76.97%), soluble glucose (21.27%) and soluble protein (37.7%). It is concluded that sheep rumen fluid enzyme has a great potential for improving nutritional quality of leucaena leaf meal of fish feed.

Keywords

Leucaena leaf meal (LLM), sheep rumen liquor enzyme, nutrient quality.

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References

Agbede JO and VA Aletor. 2004. Chemical characterization and protein quality evaluation of leaf protein concentrates from Gliricidia sepium and Leucaena leucocephala. International Journal of Food, Science and Technology 39, 253-261.

Alemawor F, VP Dzogbefia, EOK Oddoye and JH Oldbam. 2009. Enzyme cocktail for enhancing poultry utilisation of cocoa pod husk. Scientific Research and Essays 4(6), 555-559.

AOAC. 1995. Official Methods of Analysis, 16"1 ed. Chapter 4. Journal of the Association of Analytical Chemist. Washington, DC, 17-34.

Arora SP. 1989. Pencernaan Mikroba pada Ruminansia. Terjemahan R Murwani. Gadjah Mada University Press, Yogyakarta.

Bairagi A, GK Sarkar, SK Sen and AK Ray. 2004. Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilus and Bacillus circulans in formulated diets for rohu, Lobeo rohita (Hamilton) fingerlings. Aquaculture Research 35, 436-446.

Bradford MM. 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.

Cone JW. 2006. Degradation of starch in feed concentrates by enzymes, rumen fluid and rumen enzymes. Journal of the Science of Food and Agriculture 54(Issue 1), 23-34.

Czerkawski JW. 1986. An Introduction to Rumen Studies. 1" ed. Pergamon Press, New York.

Pantaya Dadik, Nahrowi dan LA Sofyan. 2005. Penambahan enzim cairan rumen pada pakan berbasis wheat pollard dengan proses pengolahan steam pelleting pada performans broiler. Media Kedokteran Hewan, 21(1).

Dubois M, KA Gilles, JK Hamilton, PA Rebers and F Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Biochem. 28, 350.

Duncan DD. 1955. Multiple range and multiple 'P test. Biometrics 11, 1-42. Garcia GW, TU Ferguson, FA Neckles and KAE Archibald. 1996. The nutritive value and forage productivity of Leucaena leucocephala. Anim Feed Sci Technol. 60, 29-41.

Hungate R. 1966. The Rumen and its Microbes. Academic Press. London and New York,

Jalilvand G, NE Odongo, S L6pez, A Nase-rian, R Valizadeh, F Eftekhar Shahrodi and KE France J. 2008. Effects of different levels of an enzyme mixture on in vitro gas production parameters of contrasting forages. Anim. Feed Sci. Tech. 146, 289-301.

Kung LJr, RJ Treacher, GA Nauman, AM Smagala, KM Endres and MA Cohen. 2000. The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance of dairy cows. J. Dairy Set. 83, 115-122.

Lend R, C Frogeot and M Lesel. 1986. Cellulose digestibility in grass carp (Ctenopharyngodon idella) and goldfish (Carassius auratus). Aquaculture 54, 11-17.

Lim C, Dominy WG. 1991. Utilization of plant proteins by warm water fish, In: Akiyama DM, Tan RKH (Eds). Proc Aquaculrure Feed processing and nutrition workshop. Thailand and Indonesia, 19-25 Sept 1991. pp 163 - 172.

Lindsay GJH. and Harris JE. (1980) Carboxymethylcellulase activity in the digestive tracts of Ash. Journal of Fish Biology 16, 219-233.

Luczkovicb JJ & Stellwag EJ. (1993) Isolation of cellulolytic microbes from the intestinal tract of the pinjzish, Lagodon rhomboides: size-related changes in * dietand microbial abundance. Marine Biologyl 16,381 -388.

Malathi V and G Devegowda. 2002. In Vitro Evaluation of Nonstarch Polysaccharide Digestibility of Feed Ingredients by Enzymes. Department of Poultry Science, University of Agricultural Sciences, Hebbal, Bangalore-560 024, India, http://ps.fass.org/cgi/reprint/80/3/302.pdf.

Moharrery A and Tirta K Das, 2002. Correlation between microbial enzyme activities in the rumen fluid of sheep under different treatments. Reprod. Nutr. Dev., 4, 513-529.

NAS. 1994. Leucaena: Promising Forage and Tree Crop for the Tropics. Second Edition. National Academy of Sciences. Washington.

Ogunji J and M Wirtta. 2001. Alternative protein sources as substitutes for fishmeal in the diet of young tilapia Oreochromis niloticus (Linn.). The Israeli Jomnal of Aquaculture 53,34-43.

Prejs A, Blaszezyk M. (1977). Relationships between food and cellulase activity in freshwater fishes. J. Fish Biol. 11, 447-452

Purnomohadi M. 2006. Peranan Bakteri Selulotik Cairan Rumen pada Fermentasi Jerami Padi Terhadap Mutu Pakan. Jurnal Protein,Vol 13, No. 2 13(2).

Saha A and Ray AK. (1998). Cellulase activity in rohu "ngerlings. Aquaculture Internationale, 281-291.

Sangeetha PT, MN Ramesh and SG Prapulla. 2004. Production of fructosyl transferase by Aspergillus oryzae CFR 202 in solid-state fermentation using agricultural by-products. Applied Microbiology and Biotechnology, 65, 530-537.

Santiago CB and Lovell RT. 1988. Amino Acid Requirement for growth of Nile Tilapia. Jounal of Nutrition 118: 1540-1546.

Scott JR. Newton SH and Katayama RW. 1982. Evaluation of sunflower meal as a soybean meal replacement in rainbow trout diets. Proceeding of Thirty-Sixth Annual Conference. South-Eastern Association of Fish and Wildlife Agencies: October 31 to November 2. 1982. Jacksonville. Florida

Stickney RR & Shumway SE. (1974). Occurrence of cellulose activity in the stomachs of e'sh. Journal of Fish Biology 6,779-790.

Sntardi, T. 1981. Sapi Perah dan Pcmbcrian Makanannya. Dep. Ilmu Makanan Ternak. Fak. Petcrnakan. Inst. Pertanian Bogor. Bogor

Twoney LN, Muske JR, Kowe JB, Choct M, Brown W, Me Connell MF, Pethick DW. 2003. The effect of increasing level of soluble non starch polysaccharide on inclusion of feed enzyme in dog diet on fecal quality and digestibility. Animal Feed Science and Technology 108; 71-82.

Vijaya GV, Gireesh T and Gajanan SB. 2002. Effect of enzymatic hydrolysis of proteins on growth of in milk. Journal of the Science of Food and Agriculture 82: 493-496.

Wedemeyer GA dan Yasutake WT. 1977. Clinical Methods for the Assesment of the Effect Environmental Stress on Fish Health. Technical Papers of the U.S. Fish and Wildlife Service. Us. Departement of the Interior 89,1-18.

Wee KL and Wang SS. 1987. Nutritive value of leucaena leaf meal in pelleted feed for nile tilapia Aquaculture 62 (2), 97-108.

Wee KL, Poe WE. 1985. Nutritive value of Leucaena leaf meal in pelleted feed for nile tilapia. Aquaculture 62, 97-108.

Widyastuti T. 2001. Detoksifikasi daun lamtoro (Leucaena leucephala) secara fisik dan kimia serta pemanfaatannya sebagai sumber pigmentasi dalam ransum ayam broiler [tesis]. Bogor: Institut Pertanian Bogor. 142

DOI: https://doi.org/10.14203/beritabiologi.v10i2.1965

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