REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.)

Yohana C Sulistyaningsih, Alex Hartana, Utut Widyastuti, Hamim Hamim, Suharsono Suharsono
| Abstract views: 353 | PDF views: 723

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.

Keywords

Engineering of expression Genetic transformation, Hd3a, rol C promoter, Jatropha curcas L.

Full Text:

PDF

References

Abe M, Y Kobayashi, S Yamamoto, Y Daimon, A Yamaguchi, Y Ikeda, H Ichinoki, M Notaguchi, K Goto and TAraki. 2005. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science 309,1052-1056.

Abdelwahd R, N Hakam, M Labhilili and SM Udupa. 2008. Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean. Af. J. Biotech. 7(8), 997-1002.

Ajay C, T Deore, and J Sudhakar. 2008. High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L: an important biodiesel plant. Plant Biotechnol Rep 2, 7-11

Bernier G, A Havelange, C Houssa, A Patitjean and P Lejeune. 1993. Physiological signal that induce flowering. Plant Cell 5,1147-1155.

Bhatt ID and U Dhar. 2004. Factors controlling micropropagation of Myrica esculenta buch. - Ham. ex D. Don: a high value wild edible of Kumaun Himalaya. Af. J . Biotech. 3 (10), 534-540.

Camellia NNA, LA Thohlrah and NAP AbduUah, 2011. Flowering and fruit set under Malaysian climate of Jatropha curcas L. Am. J. Agri. & Biol. Set. 6(1), 142-147.

Chang-wei L, L Kun, C You and S Yongyu. 2007. Floral display and breeding system of Jatropha curcas L. For. Stud. China 9(2), 114-119.

Esau K. 1977. Anatomy o f Seed Plants 2 . John Wiley & Son, New York.

George EF and PD Sherrington. 1984. Plant Propagation by Tissue Culture. Eastern Press, Reading.

Hartati SR. 2006. Persentase bunga betina sebagai salah satu faktor penentu produksi benih jarak pagar. InfoTek Jarak Pagar 1(5), 17.

He Y, V Pasapula, X Li, R Lu, B Niu, P Hou, Y Wang, Y Xu and F Chen. 2009. Agrobacterium tumefaciensmediated transformation of Jatropha curcas: factors affecting transient transformation efficiency and morphology analysis of transgenic calli. Silvae Genetica 58, 123-128.

Hsu CY, Y Liu, DS Luthe and C Yuceer. 2006. Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell 18,1846-1861

Johansen DA, 1940. Plant Microtechnique. 1. McGraw-Hill. New York. Kiong APL, YS Thing, JA Gansau and S Hussein. 2008. Induction and multiplication of callus from endosperm of Cycas revolute. Af. J. Biotech. 7(23), 4279-4284.

Kojima S, Y Takahashi.Y Kobayashi, L Monna , Ti Sasaki, T Araki and M Yano. 2002. Hd3a a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hdl under short-day conditions. Plant CellPhysiol. 43,1096-1105.

Lattanzio V, VMT Lattanzio and A Cardinal!. 2006. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: F Imperato (Ed.) Phytochemistry: Advances in Research, 23-67. Research Signpost. Kerala.

Lee JH, SM Hong, SJ Yoo, OK Park, JS Lee and JH Ann. 2006. Integration of floral inductive signals by flowering locus T and suppressor of overexpression of Constans 1. Physiol. Plant. 126,475-183.

Li M, H Li, H Jiang, X Pan and G Wu. 2007. Establishment of an Agrobacterium mediated cotyledon disc transrformation method for Jatropha curcas. Plant Cell Tiss or gan Cult. 92,173-181.

Lifschitz E and Y Eshed. 2006. Universal florigenic signals triggered by FT homologues regulate growth and flower ing cycles in perennial day-neutral tomato. J Exp Bot 57 3405-3414.

Mazumdar P, A Basu, A Paul, C Mahanta and L Sahoo. 2010 Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens mediated transformation in Jatropha curcas L. South Afr. J. Bot. 76,337-344

Murashige T and F Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15,473-497.

Newell C. 2000. Plant transformation technology. Molecular Biotechnol. 16, 53-65.

Openshaw K. 2000. A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass & Bioenergy 19 (1), 1-15 Pan J, Q Fu and ZF Xu. 2010. Agrobacterium tumefaciensmediated transformation of biofuel plant Jatropha curaa using kanamycin selection. Afr J Biotechnol. 9, 6477-6481

Raju AJS and V Ezradanam. 2002. Pollination ecology and fruiting behaviour in a monoecious species, Jatropha curcas L. (Euphorbiaceae). CurrSci. 83, 11.

Shrivastava S and M Banerjee. 2008. In vitro clonal propagation of physic nut {Jatropha curcas L.): Influence of additives. IJIB 3(1), 73-79.

Steeves TA and IM Sussex. 1989. Pattern in Plant Development 2. Cambridge Univ Press, Cambridge.

Suarez-Lopez P, K Wheatley, F Robson, H Onouchi, F Valverde and G Coupland. 2001. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature 410,1116-1120.

Sujatha M and N Mukta. 1996. Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tissue Organ Cult 44,135-141

Tanguy J and C. Martin. 1972. Phenolic compounds and the hypersensitivity reaction in Nicotiana tabacum infected with tobacco mosaic virus. Phytochemistry 11,19 - 28

Trivedi S, H Gaudani, M Gupta, N Gupta, P Paril, G Gupta, V Krishna and MP Reddy. 2009. Establishment of Agroftacterrium- mediated genetic transformation in Jatropha curcas L. Int. J. Agric. Sci. 1(2), 11-20

Vasanth K, A Lakshmiprabha and N Jayabalan. 2006. Amino acids enhancing plant regeneration from cotyledon and embryonal axis of peanut (Arachis hypogaea L.). Indian J. Crop Science 1(1-2), 79-83.748


Refbacks

  • There are currently no refbacks.