STUDI KERAGAMAN GENETIK PULAI [Alstonia scholaris (L.) R.Br.] BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA

Yuyu Suryasari Poerba
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Abstract

Alstonia scholaris (L.) R.Br. is a popular timber and medicinal tree species in Indonesia.The species is valued for its quality light wood timber and for its medicinal properties.Information on its existing genetic potential is currently lacking. The present study was carried out to optimize PCR and to screen primers among accessions collected from different part of region in Indonesia using random amplified polymorphic DNA (RAPD) markers in order to suggest appropriate primer and PCR conditions used in A.scholaris. Results showed that 26 primers generated 575 scorable bands of which 524 (92 %) were polymorphic. Fourteen highly polymorphic primers (100% polymorphic) are recorded from 48 primer used, i.e.OPA-2, OPA-03, OPA-05, OPA-06, OPA-10,OPA-12, OPA-15, OPA-18, OPA-19, OPC-03, OPC-10, OPC-12, OPC-17, and OPN-14. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method.The range of genetic distance was from 0.18-0.45.The molecular dara grouped the genotypes into three main clusters.

Keywords

Alstonia scholaris, pulai, keragaman genetik, random amplified polymorphic DNA (RAPD), primers.

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References

Carvalho VP, CF Ruas, JM Ferreira, RMP Moreira and PM Ruas. 2004. Genetic diversity among maize (Zea mays L.) landraces assessed by RAPD markers. Genetics and Molecular Biology 27, 1-16.

Delaporta SL, J Wood and JB Hicks. 1983. A plant DNA minipreparation. Version II. Plant Mol. Biol. Rep. 4, 19–21.

Dunn G and BS Everitt. 1982. An Introduction to Mathematical Taxonomy. Cambridge University Press. Cambridge.

Martin JP and JEH Bermejo. 2000. Genetic variation in the endemic and endangered Rosmarinus tomentosus Huber-Morath & Maire (Labiateae) using RAPD markers. Heredity 85, 434-443.

Mogea JP, D Gandawidjaja, H Wiriadinata, RE Nasution dan Irawati. 2001. Tumbuhan Langka Indonesia. Puslitbang Biologi.

Pither R, JS Shore and M Kellman. 2003. Genetic diversity of the tropical tree Terminalia amazonia (Combretaceae) in naturally fragmented populations. Heredity 91 (3), 307-313.

Poerba YS. 2003. Penampilan karakter agronomi dan analisis Random Amplified Polymorphic DNA (RAPD) aksesi mutan Sonchus arvensis L. Buku Kumpulan Abstrak Seminar Nasional X Persada, 124. Persada Cabang Bogor dan Badan Pengurus Pusat Persada. Jakarta 4 Juli 2003.

Rath P, G Rajaseger, CJ Goh and P Kumar. 1998. Phylogenetic analysis of Dipterocarps using Random Amplified Polymorphic DNA markers. Annals of Botany 82, 61-65.

Rohlf FJ. 1993. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 1.80. Exeter Software, New York.

Runo MS, GM Muluvi and DW Odee. 2004. Analysis of genetic structure in Melia volkensii (Gurke.) populations using random amplified polymorphic DNA. African Journal of Biotechnology 3 (8), 421-425.

Teo SP. 2000. Alstonia R. Br. In: Timber Trees: Major Commercial Timbers. Plant Resources of South-East Asia. PROSEA 5(1). I Soerianegara and RHMJ Lemmens (Eds). Bogor, Indonesia.

Tingey SV, JA Rafalski and MK Hanafey. 1994. Genetic Analysis with RAPD Markers: In: Plant Molecular Biology, 491-498. C Coruzzi and P Puidormenech (Eds.).

Widjaja EA dan YS Poerba. 2004. Pengumpulan data plasma nutfah dan genetika. Dalam: Rugayah, EA Widjaya dan Praptiwi (Ed.). Pedoman Pengumpulan Data Keanekaragaman Flora. Pusat Penelitian Biologi –LIPI. Pp. 113-140.

Williams JG, AR Kubelik, KJ Livak, JA Rafalsky and SV Tingev. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res. 18(22), 6531-6535.


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