KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia]

Sri Oetami Madyowati, Sumaryam Sumaryam, A Kusyairi, Hari Suprapto
| Abstract views: 1505 | PDF views: 1107

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.

Keywords

Koi Herpes Virus, Cyprinus carpio koi, absolute mortality, Polymerase Chain Reaction (PCR) dan transmission electron microscope (TEM) Polymerase Chain Reaction (PCR), Transmission Electron Microscope (TEM), virus-like particles

Full Text:

PDF

References

Ahne W, HV Bjorklund, S Essbauer, N Fijan, G Kurath and JR Winton. 2002. Spring viremia of carp (SVC) diseases of aquatic organism. Journal Fish Disease 52, 261-272.

Alcorn SW, AI Murray and JR Pascho. 2002). Effect on rearing temperature on immune function in sockeye salmon Oncorhynchus nerka. Fish and Shellfish Immunology 12, 303-304.

Body A, F Lieffrig, C Charlier and A Collard. 2000. Isolation of virus-like particles from koi Cyprinus carpio koi suffering gill necrosis. Journal European Association Fish Pathology 20, 87-88.

Bretzinger A, T Fischer-Scherl, M.Oumouna, R Hoffman and U Truyen. 1999. Mass mortality in koi Cyprinus carpio koi associated with gill and skin disease. Journal European Association Fish Pathology 19, 182-185.

Gilad O, S Yun, KB Andree, MA Adkison, K Way, NH Willitz, H Berkovier and RP Hedrick. 2003. Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus and effect of the temperature on mortality of experimentally infccted koi. Journal of General Virology 84, 2661-2668.

Gray ML, L Mulis, SE Patra, JM Groff and A Goodwin. 2002. Detection of koi herpesvirus DNA in tissue of infected fish. Journal of Fish Disease 25, 171-178.

Hedrick RP, O Gilad, S Yun, J V Spangenberg, G D Marty, R W Nordhaussen, SW Martin, AH Meek and P Willeberg. 2000. A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. Journal Aquatic Anim Health 12, 44-57.

Hines RS, GW Wohlfarth, R Moav and G Hulata. 1974. Genetic differences in susceptibility to two diseases among strain of the common carp. Aquaculture 3, 187-197.

Neukirch M, K Bottcher and S Bunajirakkul. 1999. Isolation of virus from koi with altered gill. Journal European Association Fish Pathology 19, 221-224.

Ornamental Aquatic Trade Association - OATA. 2001. Koi Herpes Virus (KHV), Washington, DC.

Pererlberg A, M Smirnov, M Hutoran, Y Bejerano and M Kotler. 2003. Epidemioloical description of a new viral disease afflicting cultured Cyprinus carpio in Israel. Israeli Journal of Aquaculture 55 (1), 5-12.

Pikarsky E, A Ronen, J Abramowitz, B Levavi-Sivan, M Hutoran, Y Shapira, M Steinitz, A Pererlberg, D Soffer and M Kotler. 2004. Patogenetis of acute viral disease in fish by carp interstitial nephritis and gill necrosis virus. Journal of Virology 78, 9544-9551.

Ronen A, and IC Liao. 2003. Effect of dissolved oxygen, temperature and salinity on the oxygen consumption of the grass shrimp. Proceeding of the First Asian Fisheries Forum, 641-643. JL Maclean, LB Dixon and LV Hossilos (Editors). Asian Fisheries Society. Manila. Philippines.

Sambrook M, H Towbin, T Staehelin and J Gourdon .1989. Electrophoresis transfer of protein from polyacrylamide gells to nitrocellulose sheets: Procedure and some applications. Journal of Fish Disease 76, 4350-4354.


Refbacks

  • There are currently no refbacks.