PENGARUH SUKROSA DAN GLUTAMIN PADA KULTUR ANTER Solatium khasianutn CLARKE

Djunaedi Gandawidjaja
10.14203/beritabiologi.v4i2&3.1285
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Abstract

Effect of Sucrose and Glutamine on Anter Culture ofSolanum khasianum Clarke, Djunaedi Gandawidjaja.Anther ofSolanum khasianum Clarke were cultured on MS medium enriched with sucrose (30, 60, 75, 90 g/l)combined with glutamine (0, 200, 400, 600 mg/l)or with sucrose (60 g/1) combined with glutamine at low concentrations (0, 0.5,2.5, 5.0,10.0,20.0,50.0 mg/l).Callus formation was affected by the interaction of sucrose and glutamine. Callus formation decreased with the increasing concentration of sucrose or glutamine. Combination of sucrose and glutamine was unable to regenerate planletfrom the callus.

Keywords

Solanum khasianum, anther, sucrose, glutamine, callus

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References

Dunwell JM and Sunderland N. 1973. Anther culture of Solanum tuberosum L. Euphytica 22: 317-323.

Gandawidjaja D. 1992. Petumbuhan dan Perkembangan Anter Soalnum khasianutn Clarke dalam Kultur in-vitro. Disertasi S3, Program Pascasarjana-Institut Pertanian Bogor,lll halaman.

Gandawidjaja D. 1993. Pembentukan kalus dan regenerasi tanaman dari anter Solanum khasianutn Clarke dalam kultur in-vitro. Makalah pada SetninarNasionalBiologi XI, Ujungpandang,-20-21 Juli 1993.

Gresshoff PM and Doy GH . 1972. Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta (Bed) 107: 161 - 170.

Hammerschlag F A. 1983. Factors influencing the frequency of callus formation among cultured peach anthers. HortSd. 18:210 - 211.

HincheeMAW and Fitch MM. 1984. Culture of isolated microspores of Saceharum spontaneum. Z.PflPhjsioL Bd. 113 S.: 305 - 314.

Hu H and Zeng JZ . 1984. Development of new varieties via anther culture. In: Handbook of Plant Cell Culture Vol 3 , pp. 65 - 90. Macmillan Co.New York.

Keller WA, Rajhathy T and LacapraJ . 1975. In vitro production of plants from pollen in Brassica campestris. Can. J. Genet. Cytol. 17: 655 - 666.

Martin MJC and Millo EP . 1981. Anther and pollen grain culture of rice [Oryza sativa L.). Euphytica W: 541 -546. Nitsch JP and Nitsch C. 1969. Haploid plants from pollen grains. Science 163: 85 - 87.

Owen HR, Veilleux RE, Haynes FL and Haynes KG . 1988. Variability of critical photoperiod of tuberization and tuber yield among monoploid anther-derived genotype of Solanumphureja. J. Amer. Soc. Hort. Sd. 113: 755 -759.

PierikRLM. 1987. In vitro Culture of Higher Plants. Martinus Nijnhoff Publishers, Dordrecht etc. 344 pp.

Powell W. 1988. The influence of genotype and temperaturetreatment on anther culture response in barley (Hordeum vulgare L.). Plant Cell Tissue and Organ Culture 12:291 -297.

Powell W and Uhrig H. 1987. Anther culture of Solanum genotypes. Plant Cell Tissue and Organ Culture 11: 13 - 24.

Redenbaugh MK, Westfall RD and Karnosky DF. 1981. Dihaploid callus production from Ulmus atnericana anthers. Bot. Ga% 142: 19 - 26.

Reynolds TL. 1986. Pollen embryogenesis in anther cultures of Solanum carolinense L. Plant Cell Reports 5: 273 -275.

Sudiarto. 1981. Percobaan penenanaman tanaman sumber diosgenin dan solasodin (Experiment on cultivation of diosgenin and solasodin yielding plants). Makalah dalam Seminar Nasional Produksi Bahan Baku Kontraseptif Oral, 23-25 Juni.

Sudiarto dan Rosita MD . 1982. Usaha penyediaan bahan baku kontraseptif oral. Suatu usaha swasembada dalam rangka mensukseskan Program Keluarga Berencana Nasional. Jurnal PeneMan & Pengembangan Pertanian 1 (I) :41 - 44.

Sunderland N and Roberts M . 1977. Anther and pollen culture. Nature 270: 236 - 238.

Thurling N and Chay PM . 1984. The influence of donor plant genotype and environment on production of multicellularrnicrospores in cultured anthers of Brassica napus ssp. oleifera. Ann. Bot. 54: 681 - 693.

DOI: https://doi.org/10.14203/beritabiologi.v4i2&3.1285

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