KULTUR JARINGAN KUNIR PUTIH (Kaempferia rotunda L.)

Djadja Siti Hazar Hoesen
10.14203/beritabiologi.v4i4.1269
| Abstract views: 1819 | PDF views: 1495

Abstract

Shoot cultures of Kaempferia rotunda L.were established from rhizome segment.In the 1st experimen these explants were planted on Gamborg/B5 medium that supplemented with BA concentrations were (0, 0.5, 1 and 2) mg/l and/or kinetine were (0, 2 and 4) mg/l.In 2nd experiment these explants planted in Murashige and Skoog medium (MS).This medium supplemented with BA concentrations (0, 2 and 4) mg/l and/or NAA (0 and 1) mg/l.The result in the 1st experiment showed that the best proliferated shoots was from the culture that supplemented with BA 1 mg/l and kinetine 4 mg/l, while in the 2nd experiment the best proliferated shoots was from the culture that supplemented with BA 4 mg/l and NAA 1 mg/l.These shoots were then subcultured on MS liquid medium supplemented with BA (5 mg/l) and MS agar medium was supplemented with BA (2 mg/l) + 2iP (0.5 mg/l) + thidiazuron (0.01 mg/l) + NAA (0.5 mg/l).The cultures could induce the shoots number and produce the morphological plantlets for acclimatization,and acclimatization successed on soil and compost mixed medium in ratio 1:1.

Keywords

Zingiberaceae, Kaemnferia rotunda, kultur jaringan, Murashige and Skoog/MS, Gamborg/B5.

Full Text:

PDF

References

Backer CA and van Den Brink RCB. 1968. Flora of Java Vol III Groningen The Nederlands.

Bates S. Preece JE. Navarette NE. Van Samberk JW and Gaffney GR. 1992. Thidiazuron stimulates organogenesis and somatic embryogenesis in white ash (Fraximus americana L) Plant Cell Tissue and Organ Culture 31 : 21-29.

Gerorge EF and Sherington PD. 1984. Plant propagation by tissue culture. Handbook and Directory of Commercial Laboratories.

Hoesen DSH dan Poerba YS. 1993. Perbanyakan tanaman jahe merah (Zingiber officinale Rose. var. Roebra) dengan teknik kultur jaringan. Prosiding Seminar Hasil Penelitian dan Pengembangan Bioteknologi : 324-328. Pusat Penelitian dan Pengembangan Bioteknologi - LIPI.

Hoesen DSH. 1996. Pembentukan tunas kencur secara in-vitro . Warta Tumbuhan Obat Indonesia vol. 3 no. 2 : 21-23. Kelompok Kerja Nasional Tumbuhan Obat Indonesia Jakarta.

Ikeda RL and Tanabe MJ. 1989. In-vitro subculture application for ginger. Hort. Science 24: 142-143.

Imelda. M. 1993. Penyediaan bibit jahe gajah dengan teknologi biak jaringan . Prosiding Seminar Hasil Penelitian dan Pengembangan Bioteknologi : 335-340. Pusat Penelitian dan Pengembangan Bioteknologi- LIPI.

Lillo. C. 1989. Effects of media components and evirontmental factors on shoot formation from protoplast derived calli of Solanum tuberosum. Plant Cell Tissue and Organ Culture 19: 103-111.

Murashige. T. & F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture . Physiology Plantarum 15 : 473-497,

Mariska I, Seswita D dan Gati E. 1996. Aplikasi kultur jaringan untuk perbanyakan klonal tanaman kencur. Warta Tumbuhan Obat Indonesia, vol. 3 no. 2. : 11-13.

Pierik RLM. 1987. In-vitro culture of higher plants. Martinus Nijhoff Publisher Ordrecht/Boston/Lancaster.

Preece JE. 199S. Can nutrients salts partially substitute for olant growth regulators (?). Plant Tissue Culture and Biotechnology vol.1. no. 1 : 26-27.

Quisumbing E. 1951. Medicinal Plants of the Philippines . Republic of the Phlippines Department of Agriculture and Natural Resources. Manila Bureau Printing.

Skoog Fand Miller CO. 1957. Chemical regulation of growth and organ formation in plant tissue cultured in-vitro. Symposium society Experimental Biology 15 : 118-137.

Sastrapradja S. 1977. Ubi-ubian. ProyekSumber Daya Ekonomi Lembaga Biologi Nasional-LIPI. Bogor.

Utami KP. 1997. Motif-motif eksotik daun kencur hias. Trubus No. 334 Tahun XVIII 1 September 1997.

Van Niewkerk JP. Zimmermann RH and Fordham I. 1986. Thidiazuron stimulation of apple shoot proliferation in-vitro. Hort. Science 21 : 516-518.

Wareing PF and Philips IDJ. 1981. Growth differentiation in plants. Pergamon Press New York.

Yelnititis. 1996. Pengaruh BA, thidiazuron dan auksin (IAA & IBA) terhadap multiplikasi tunas dan perakaran in-vitro daun encok. Prosiding Simposium Nasional I Tumbuhan Obat dan Aromatik (APINMAP) : 278-283.

DOI: https://doi.org/10.14203/beritabiologi.v4i4.1269

Refbacks

  • There are currently no refbacks.